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Investigating the effect of temperature on the action of trypsin.

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Introduction

Biology coursework Aim: Investigating the effect of temperature on the action of trypsin Apparatus; 1 water beaker 10 test tubes 1 test tube rack 10 pieces of photographic film 30cm of trypsin 10cm of alkali buffer 1 stopwatch 2 pipettes Method: Firstly place all 10 test tubes into the test tube rack. Pour 3cm of trypsin into each tube and add 1cm of alkali (pH9.7). Then collect the water for the beaker and heat to 50degrees for the first experiment. Place the first 2pieces of photographic film into the 2tubes and put into the water beaker. Start the stopwatch as soon as the tubes enter the water. Record the results as soon as the film goes clear. Do the same for 40, 30, 20 and 10 degrees. When all the tests have been done, record the results in a table. Fair test: To ensure that my experiment is a fair test I am going to keep the same: * The amount of trypsin used each time * The size of the photographic film * The point at which the stopwatch is started * The amount of water in ...read more.

Middle

1 10 531 2 10 536 533 3 20 435 4 20 442 438 5 30 319 6 30 331 325 7 40 246 8 40 238 242 9 50 270 10 50 286 278 Conclusion: My graph provides me with the information that trypsin works best in alkaline conditions, at the temperature of 40degrees. This is because, as predicted, it is closest to the body temperature (37degrees). Trypsin is found as an enzyme in the small intestine, hence the best result being 40degrees. There is a pattern in my results because, as the graph shows, the farther away from body temperature, the less effective the trypsin is. This is due to the lack or too much movement of the trypsin particles. Too high - the trypsin is denatured, too low - not enough particle collisions. The enzyme and substrate particles vibrate slowly at a low temperature which means fewer enzymes and substrate complexes formed. The same with a higher temperature but because there are more collisions, the enzyme will change its shape and the substrate will no longer fit. Evaluation: I believe that my results are pretty accurate. ...read more.

Conclusion

This is because trypsin is an enzyme found in the body. If I was to do this experiment again I would: * Use a water bath instead of a beaker, so that the water would remain at a constant temperature at all times. * Make sure that all of the pieces of photographic film are precisely the same size, as that may have affected my results slightly. * Record results at 60 and 70 degrees to prove that my theory works (the farther away from 37degrees the temperature is, the longer it takes) * Take more results around 37degrees to see if the trypsin does actually work best around body temperature. This would cause the curve on my graph to fall lower and curve more. This I have highlighted on my graph. * Find a more accurate way of testing that the photographic film has gone clear. I could possibly take it out of the beaker after the experiment, and shine light through it and see if it is totally clear. This would help me to record my results accurately. To gain a more accurate graph, I could maybe use units going up in 5degrees. The points would be easier to defy and would be closer together. ...read more.

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