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Investigating the inhibition of copper sulphate between an enzyme and substrate.

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Introduction

Investigating the inhibition of copper sulphate between an enzyme and substrate Introduction In this investigation I will be carrying out an experiment to see how the addition of copper sulphate to the reaction between hydrogen peroxide and carrots is effected. This is a simple model showing how inhibition (copper sulphate is inhibitor) takes place between an enzyme (carrot) and substrate (hydrogen Peroxide). It will most likely show the effect of a competitive inhibitor as the more inhibitor you add to the reaction between the enzyme and substrate, the slower the reaction between the substrate and enzyme will slow down. I will know how well the reaction (enzyme and substrate binding) is going by collecting the amount of gas (oxygen) given off in the reaction. Obviously if there is a lot of gas given off then the reaction will be faster than if the reaction is slow. I will be going through what my investigation is about and developing a prediction about my investigation. I will then go through some information about information and inhibition and tell you what I know about them. After that I will introduce you to my pre-tests and how I came about to choose the method that I used in the end. I will then talk about what I will need to take account for when doing my investigation, such things as what I'll need to carry out the investigation, what precautions I will take and what things will be done throughout the tests that I do. I will include tables and graphs to show my results and finally conclude my results and finish my investigation by evaluating how well my investigation went. ...read more.

Middle

I will keep doing this for approximately 3 and a half minutes. I plan to repeat the test for each different concentrations of copper sulphate as this will increase the reliability of my work. Apparatus After finding out what method I was going to use I then began to consider about what apparatus I would require and came up with the following list: * Carrot gratings (4g per test) - Acts as enzyme in investigation. * Hydrogen peroxide (5ml per gram) - Acts as substrate in investigation. * Copper sulphate (1% - 0%) - Acts as inhibitor in investigation. I will use different concentration to test inhibition. * 2 Measuring cylinders - to measure the amount of hydrogen peroxide and copper sulphate * Petri dish - to hold 4g carrot gratings in ready for using in the investigation for each test. * Weighing pan - to weigh carrot gratings (4g approx) * Glass syringe - will collect gas given off by the reaction between the enzyme and substrate (carrot gratings and hydrogen peroxide). * Clamp stand - to hold glass syringe to stop it from rolling around and breaking. * 2 Beakers - to hold hydrogen peroxide and copper sulphate close to apparatus. * Test tube - To put carrot gratings, hydrogen peroxide and copper sulphate in for reaction to take place. * Bung and rubber tube - Bung will go over test tube and the rubber tube will be connected to the glass syringe where oxygen gas given off will be collected. * Test tube rack - simply to keep test tube in one place and stable so it doesn't go anywhere and end up breaking. ...read more.

Conclusion

* Some misreadings may have occurred; this is due to the available apparatus. The investigation could be done with more accurate apparatus. * The anomalous results on my initial rate of reaction graph may be my error of reading the gradients wrong on the other graphs and if done correctly the graph may show a pattern. Things I have to account for if the investigation was to be repeated: * Make sure I know how to do initial rate of reaction graphs because I did the one in my investigation on what I thought was correct. * More sources for information relating to investigation giving a wider and more understanding of the investigation. * If possible use more accurate apparatus to stop some human errors. Accuracy of results: I think that my results were accurate but not very. They showed a pattern or trend that told me that as the copper sulphate concentration increased the reaction (release of oxygen gas) became slower due to inhibition taking place. The accuracy of the apparatus is ok but I think that even more accurate apparatus could have been used to get correct amount of liquids or solutions. The gas syringe readings were pretty accurate but there were times when a decision had to be made about the results because the reading may have been between points, e.g. in between 12ml and 13ml. The results aren't very reliable as I didn't repeat any of the tests. I only have one set of results which makes the results unreliable as I don't know if I would get different values which are completely different to the original ones and have large differences. ?? ?? ?? ?? Balwant Flora 10/05/2007 1 ...read more.

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