Results:
The table showing the size of clear spots formed with different substance
Discussion:
The size of clear spot follows the order: Pineapple> Biological washing > Papaya >meat tenderizer powder (Attack)> water
Therefore, the enzyme activity follows the same order.
The above substances contain protease that can break down protein gel and caused the silver salts to detach form the films, leaving clear spots.
Water should contain no protease but there was clear spot at the area around water. The possible reason should be pineapple juice that was added next to water mixed with it and cause the digestion the protein gel.
Errors:
The sizes of drops of solution were different.
Evaporation of solution might change the concentration of enzyme.
The Petri dish might be tilted during transfer.
Improvement:
Use droppers of the same pore size.
Biological significant:
Pineapple and papaya may be better than meat tenderizer in breaking down protein fibres in meat and tenderize the meat better.
Conclusion:
The enzyme activities of different substance in breaking down protein are different and follow the order: Pineapple> Biological washing > Papaya >meat tenderizer powder (Attack)> water.
