Production of lactase free milk.

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Unit 6: production of lactase free milk.

The aim;

Cats and many humans are unable to digest milk containing lactose. The reason foe this is they do not have the enzyme lactase which breaks down lactose in their digestive system. The reason for me to carry out this experiment is to produce milk which doses not contain lactose, which is safe for cats and humans that are lactose intolerant.

In this experiment:

I have used the enzyme lactase which digests lactose (milk sugar)

I have immobilised the lactase enzymes by trapping it within beads of sodium alginate.

Also I will use the immobilised lactase enzyme to make lactose free milk.

The equipment:

> Tea strainer

> Clinistix 2x

> Small piece of nylon gauze

> 10cm³ plastic syringe

> 20cm³ plastic syringe

> 100cm³ beaker

> 250cm³ beaker

> retort stand

> clamp

> 2cm³ lactase

> 8cm³ of 2% sodium alginate solution

> 100cm³ of 1.5% calcium chloride solution

> lab coat

> spatula