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Sectors of leisure and recreation for football and swimming

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Investigation to discover the affect of concentration on enzyme action Aim: The aim of this investigation is to find out exactly what effect, concentration of enzyme has on the rate at which Amylase breaks down starch. Background: Enzymes are biological catalysts and are used to speed up reactions, usually these reactions are either breaking things up, (catabolic) or they are building things up (anabolic) the enzymes are super specific and an enzyme's active site is only the right shape so that one specific reaction can be carried out by it. Enzymes are made of proteins and so they have a very complicated shape. They are therefore highly specific in the reactions that they take part in so an enzyme can only react with molecules of one substrate. ...read more.


to be reacted. Prediction and hypothesis: As the concentration of enzyme increases we would expect to see that the rate of reaction should increase in proportion to start off with. This is because at low enzyme concentration there is great competition for the active sites and the rate of reaction is low. However, as the enzyme concentration increases, there are now more active sites that can be filled with substrate and the reaction can proceed at a faster rate. This is shown in the collision theory in which it is stated that as the concentration increases there are more molecules hitting each other which leads to more reactions being carried out over a shorter amount of time because as there are more enzyme molecules the chance of a substrate hitting an enzyme becomes much higher. ...read more.


I would then check the temperature of the solution and place it into the beaker of water to keep the solution at a constant temperature. This would make sure that the rate of reaction is not affected by temperature and so this is a fair test. I wouls then measue out more starch solution into some more boiling tubes and place them in the water bath. i would do this again until I have six boiling tubes at the same temperature with the same amount of the same starch solution. Once all the tubes are at the same temperature, measured accurately with the thermometer, I would place them in the test tube rack. Then I wou ...read more.

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