• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Studying the Effect of Salt on Cress Germination

Extracts from this document...

Introduction

PLANNING Initial Method 1. Prepare 8 sterile Petri dishes with a perfectly fitting circle of cotton wool and filter paper, this will sit on top of the wool 2. A control dish must also be set up using the same steps as above 3. Weigh out 8 different salt measures, at 0.25, 0.5, 0.75, 1, 1.25, 1.5 and 1.75 4. Measure out 8, 50ml beakers of distilled water 5. Add the one measure of salt into a beaker (1 beaker for each weight) and stir until the salt is dissolved and cannot be seen 6. Add one drop of Plant nutrient growth (e.g. baby bio) to each solution 7. Add each solution into individual Petri dishes which were made up earlier on, make sure the cotton wool and filter paper are allowed a small amount of time to absorb as much water as possible before the next step 8. Add 10 Cress seeds to each of the 8 solutions and place the lid on the dish 9. Place the dishes in are area which is well lit by natural light 10. Check the dishes each day for a week and top up each dish with the same solution if it is becoming dry, add the same amount to each dish (record what you add) ...read more.

Middle

I controlled the samples of cress water and salt by ensuring they were all measured correctly and from the source. I used the same type of cress and salt which were taken from one batch. The water was also all used from the same bottle. Table of results (preliminary) SALT (grams, g) SALT CONCENTRATION (%) NUMBER OF SEEDS GERMINATED (%) 0.25 0.5 100 0.5 1 70 0.75 1.5 60 1 2 40 1.25 2.5 0 1.5 3 0 1.75 3.5 0 0 0 100 Figure 2 - preliminary results The results I have recorded are sufficient to use in a spearman rank coefficient data test as I am looking for a correlation and trend and have taken a suitable amount of repeats and tests to gain a result from the stats test. I also have no anomalies in my original results as they all appear to follow a trend Statistical Data Test - Spearman Rank Coefficient For my main experiment, I have chosen to analyse the results using a Spearman's Rank Correlation Coefficient test. This should allow me to assess whether there is significant correlation between the concentration of salt (NCaL) and the germination of seeds. This test is appropriate as I am taking readings for 8 different concentrations, and both of the variables recorded (germination % and concentration) ...read more.

Conclusion

11. Repeat this process again so that you run two sets of 8 salt concentrations OBSERVING AND RECORDING Table of Results SALT (grams, g) SALT CONCENTRATION (%) NUMBER OF SEEDS GERMINATED (%) 0.05 0.1 92.5 0.2 0.4 65 0.35 0.7 40 0.5 1 20 0.65 1.3 12.5 0.8 1.6 0 0.95 1.9 0 0 0 97.5 Figure 7 - Main investigation results The above results show a general correlation indicating that increased salt concentrations reduce the germination of cress seeds, however I will analyse this later Statistical Data Test - Spearman Rank Coefficient Category Data 1 Rank R1 Data 2 Rank R2 d = (R1 - R2) d2 1 0 1 97.5 8 -7 49 2 0.1 2 92.5 7 -5 25 3 0.4 3 65 6 -3 9 4 0.7 4 40 5 -1 1 5 1 5 20 4 1 1 6 1.3 6 12.5 3 3 9 7 1.6 7 0 1.5 5.5 30.25 8 1.9 8 0 1.5 6.5 42.25 n 8 Sum 0 167 Figure 8 - Main investigation statistical test The spearman rank correlation coefficient equalled - 0.98 and the critical value were 0.64 giving a significant correlation (which was negative) therefore disproving my null hypothesis "salt has no effect on the germination of cress seeds." The results show that the larger the salt concentration gets the lesser the cress seed germination is. INTERPRETATION AND EVALUATION Figure 9 - Main Investigation results graph. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Energy, Respiration & the Environment section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

4 star(s)

A well conducted investigation with clearly identified variables. Appropriate statistical analyses successfully carried out but their interpretation should refer to levels of significance, chance and probability.

4 Stars

Marked by teacher Adam Roberts 25/10/2014

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Energy, Respiration & the Environment essays

  1. Marked by a teacher

    Effect of Anaerobic Respiration On Yeast

    5 star(s)

    in the water bath and the temperature was allowed to regulate for 10minutes, this was measured using a timer. 6. Once this 10minutes had elapsed the bung was put onto the conical flask and the tubing put up the burette to.

  2. Marked by a teacher

    An investigation into the effect of different sugars on respiration in yeast.

    5 star(s)

    Unlike other disaccharides, sucrose is not a reducing sugar. A reducing sugar is a sugar which has the capability of reducing various inorganic ions. The disaccharide is held together by a glycosidic bond between carbon 1 of glucose and carbon 2 of fructose. It is most commonly known as normal table sugar and is less reactive than glucose.

  1. Marked by a teacher

    Biology coursework investigation: Comparing the length of ivy leaves (Hedera helix) in areas of ...

    4 star(s)

    This number of samples requires a student's-t test to be carried out in order to compare the two sets of data. The tables below display the measurements in terms of cumulative mean for both sets of leaves. Table 1: Measurements of Hedera helix in the brighter area Leaf number Length (cm)

  2. What effect does substrate have on respiration in yeast?

    This should be carried out for all five substrates. These controls are needed to prove that it is the substrates given to the yeast solution that causes the yeast to respire and produce carbon dioxide. They would also demonstrate that yeast by itself or just the substrate can not produce

  1. Daphnia Investigation

    Use fine focusing for any fine adjustments. Adjust the condenser and light aperture to ensure the best illumination. Hypothesis I expect that the presence of caffeine will cause an increase in heart rate. This is because the caffeine will act as a stimulant and signal heat cells in the body to respire at a faster rate.

  2. An investigation into the effects of temperature on the rate of anaerobic respiration of ...

    10 3.7 10.9 13.5 15 4.7 14.1 18.5 20 5.6 17.7 21.9 25 6.2 20.9 24.7 30 7.3 23.1 27.6 35 8.1 26.2 29.5 40 9.4 27.9 31.6 45 9.8 29.7 33.2 50 10.7 30.9 34.2 My average results suggest that 25 minutes in the water bath at 20 �C,

  1. Vitamin C Practical Experiment Write Up

    I will measure 1% DCPIP 1cm(3) from a beaker to a pipette, with another pipette I will add the 1% vitamin C solution which is the control of the experiment 1cm(3) at a time to a test tube, each time 1cm(3)

  2. Effects of exercise on tidal volume and breathing rate

    The increase in tidal volume comes as an expense of inspiratory reserve volume. The frequency of breathing may increase to 40 to 50 breaths per minute in a healthy individual. The increase of tidal volume (VT) during exercise occurs as a result of both an increase in end-inspiratory and a

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work