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The aim of this practical is to investigate on the different amounts of Vitamin C in a variety of juices and research on how it is affected when stored in its natural state or diluted.

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Introduction

Which type of fruit juice provides the most Vitamin C? Aim The aim of this practical is to investigate on the different amounts of Vitamin C in a variety of juices and research on how it is affected when stored in its natural state or diluted. To enable this, a reasonable amount of fruit juices ( apple, black-currant, lemon and orange)where all added into pre-prepared wells as this will help us find out which of the fruit juices mentioned above contained the most vitamin C. This practical will help develop key practical skills and improve data representation. Introduction Vitamin C (Ascorbic Acid) is an essential nutrient found in fresh fruits or vegetables like: lemons, oranges broccoli and Brussels sprouts. Vitamin C acts as an anti-oxidant to prevent plaque in the blood vessels and ensures that the central nervous system is functioning properly. Vitamin C is one of the most essential nutrients required for the body to function properly as it aids the formation of collagen which holds connective tissues together. Although this experiment does not oppose to any unethical issues, some may state otherwise due to fair trade as fresh fruits were used to carry out the investigation so alternative sources (Juices with artificial flavours) ...read more.

Middle

Wash hands before and after practical. Correct disposal of bio-hazard material. * Health Avoid contact with any chemical substance you work with. Don't eat nor drink in the lab. * Ethics There are no causes for alarm Although some might claim otherwise Due to fair trade. Justifying the validity and reliability of the chosen method Repeats should be kept at a minimum and only repeated when necessary and students must familiarise themselves with the equipment before the start of every practical. Measurements taken should be accurate and students may cross-check with each other to avoid too many anomalous results. However, anomalous results should be investigated if they occur too frequently. Suitable apparatus and experimental procedures should be followed strictly to ensure valid results and precision and only the most precise equipment should be used to make the test fair and accurate. Results A table to show the area of colour change produced by solutions with varying vitamin C concentration % Vitamin C/% Area of the circle/cm Test 1 Area of the circle/cm Test 2 Area of the circle/cm Test 3 Area of the circle/cm Fruit juices Area of the circle/cm Test 1 Area of the circle/cm Test 2 Area of the circle/cm Test 3 Av. ...read more.

Conclusion

Other sources of error are lack of concentration and personal bias and lack of callipers meant that the results may not have been as precise and accurate. LIMITATIONS One of the most common factors was lack of time and callipers were not available so the numbers of repeats were limited. REDUCING ERRORS Extra care should be taken when using Vitamin C solution and callipers should be used when repeating the experiment as it would improve accuracy. 'Used' agar gel plate should be labelled clearly so they are not disposed off or used carelessly when a separate group is carrying out the same practical. CONCLUSION In conclusion, the overall results disagree with the prediction that orange juice contains the most Vitamin C. This was due to the lemon juice been proven to contain the most vitamin C since it provided the most results when the Vitamin C solution was added to it and also lemon juice is most known for its medicinal purposes. IMPROVEMENTS Callipers will improve accuracy and also more repeats carried out in the practical will ensure reliability and the students would have more time to cross-check every measurement. FUTHERWORK It would be necessary to carry out the experiment using 2%of Vitamin C solution and also to repeat the 2nd and 3rd trials carried out earlier to produce more accurate results. RESOURCES As Salter's' Nuffield Biology www.course-workinfo.co.uk www.ummm.edu/altmed/articles/vitamin C-0003339.htm ...read more.

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