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The effect of concentration of enzyme and enzyme substrate in the rate of an enzyme catalysed reaction.

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The effect of concentration of enzyme and enzyme substrate in the rate of an enzyme catalysed reaction As part of the AS level biology coursework, there was an investigation to complete which is titled 'The effect of concentration of (i) Enzyme and (ii) enzyme substrate on the rate of an enzyme controlled reaction. Method 1: - The effect of concentration of enzyme on the rate of an enzyme catalysed reaction The set up of the experiment is as shown above with clamps set up correctly and everything put in the correct place. When the apparatus was set up completely the experiment was started. Firstly there had to be a results table in which the results was concluded and after this was done, a preliminary run was executed in which the investigator could get a feel for the experiment and learn how it performed and as usual the run was a negated test. The actual experiment then had to have been carried out. The syringe from the needle was removed and then occupied with hydrogen peroxide before being re-established with the needle. The bung was then taken out of the test tube and filled in with yeast suspension before putting the bung back on, a check had to have been made to make sure there were no loose connections and the clip on the inverted barrel was tightly screwed so there was no oxygen gas left on the barrel. ...read more.


The reasons for the same amount of product being produced after each run being the same to do with enzyme concentration (yeast). During each run the enzyme was being increased and the substrate was being kept constant. This means that there were a lot more enzymes working with limited substrate concentration and so the enzymes will have produced the products more quickly if there was more of it. For example if 10 miners (enzyme) were mining a small coal mine (enzyme) it would probably take some time for the coal (product) to be retrieved. But if there were 15 miners then it would take them a shorter period of time to mine and 20 miners would take them even a shorter time and so on. But the product, which is the coal, will always remain the same because that's all the coal the mine has to offer. Method 2: - The effect of concentration of enzyme substrate on the rate of an enzyme catalysed reaction. In method 2, the amount of substrate (hydrogen peroxide) was increased after each run and the amount of enzyme (yeast) was kept constant during each run. The results concluded were that the amount of product collected was higher in each run than previously. The reason behind this is that the volume of the hydrogen peroxide (substrate) was being increased each time yet the yeast (enzyme) ...read more.


of the bung the same problem may occur by the investigator moving the needle vigorously causing in holes being present from which the gas can escape. Another error would be that the clip which was connected to the rubber tubil may not have been very tight in connection with the rubber tubil, consequentially gas bubbles would appear in the inverted barrel before the reaction took place which would muddle up the results, also the investigator should remember to release the clip after each run so that no gas would be left behind with the inverted barrel. In addition the beaker of water in which the inverted barrel was placed, may have been minutely smaller or the same size as the inverted barrel so thus the barrel would not fill up which resulted in gas being in the barrel already once the reaction was started. Another detrimental error was that when taking up the hydrogen peroxide using the syringe, sometimes bubbles would appear which made the measurement of how much hydrogen peroxide was used in each run, quite difficult. The final error noted was that in the yeast suspension there was quite a lot of froth built up. So when measuring how much yeast was needed, the volume recorded was not accurate because if u recorded 10cm3 of yeast it may have 12cm3 of yeast. The effect of concentration of enzyme and enzyme substrate in the rate of an enzyme catalysed reaction Ali Omar Page 1 ...read more.

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