• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The effect of concentration of enzyme and enzyme substrate in the rate of an enzyme catalysed reaction.

Extracts from this document...

Introduction

The effect of concentration of enzyme and enzyme substrate in the rate of an enzyme catalysed reaction As part of the AS level biology coursework, there was an investigation to complete which is titled 'The effect of concentration of (i) Enzyme and (ii) enzyme substrate on the rate of an enzyme controlled reaction. Method 1: - The effect of concentration of enzyme on the rate of an enzyme catalysed reaction The set up of the experiment is as shown above with clamps set up correctly and everything put in the correct place. When the apparatus was set up completely the experiment was started. Firstly there had to be a results table in which the results was concluded and after this was done, a preliminary run was executed in which the investigator could get a feel for the experiment and learn how it performed and as usual the run was a negated test. The actual experiment then had to have been carried out. The syringe from the needle was removed and then occupied with hydrogen peroxide before being re-established with the needle. The bung was then taken out of the test tube and filled in with yeast suspension before putting the bung back on, a check had to have been made to make sure there were no loose connections and the clip on the inverted barrel was tightly screwed so there was no oxygen gas left on the barrel. ...read more.

Middle

The reasons for the same amount of product being produced after each run being the same to do with enzyme concentration (yeast). During each run the enzyme was being increased and the substrate was being kept constant. This means that there were a lot more enzymes working with limited substrate concentration and so the enzymes will have produced the products more quickly if there was more of it. For example if 10 miners (enzyme) were mining a small coal mine (enzyme) it would probably take some time for the coal (product) to be retrieved. But if there were 15 miners then it would take them a shorter period of time to mine and 20 miners would take them even a shorter time and so on. But the product, which is the coal, will always remain the same because that's all the coal the mine has to offer. Method 2: - The effect of concentration of enzyme substrate on the rate of an enzyme catalysed reaction. In method 2, the amount of substrate (hydrogen peroxide) was increased after each run and the amount of enzyme (yeast) was kept constant during each run. The results concluded were that the amount of product collected was higher in each run than previously. The reason behind this is that the volume of the hydrogen peroxide (substrate) was being increased each time yet the yeast (enzyme) ...read more.

Conclusion

of the bung the same problem may occur by the investigator moving the needle vigorously causing in holes being present from which the gas can escape. Another error would be that the clip which was connected to the rubber tubil may not have been very tight in connection with the rubber tubil, consequentially gas bubbles would appear in the inverted barrel before the reaction took place which would muddle up the results, also the investigator should remember to release the clip after each run so that no gas would be left behind with the inverted barrel. In addition the beaker of water in which the inverted barrel was placed, may have been minutely smaller or the same size as the inverted barrel so thus the barrel would not fill up which resulted in gas being in the barrel already once the reaction was started. Another detrimental error was that when taking up the hydrogen peroxide using the syringe, sometimes bubbles would appear which made the measurement of how much hydrogen peroxide was used in each run, quite difficult. The final error noted was that in the yeast suspension there was quite a lot of froth built up. So when measuring how much yeast was needed, the volume recorded was not accurate because if u recorded 10cm3 of yeast it may have 12cm3 of yeast. The effect of concentration of enzyme and enzyme substrate in the rate of an enzyme catalysed reaction Ali Omar Page 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Peer reviewed

    Effect of enzyme concentration on rate of reaction

    4 star(s)

    The enzyme then catalyses the conversion of substrate into product(s) which depart from the active site. The enzyme molecule is then free to bind with more substrate molecules. For anabolic reactions, this is achieved by holding the substrate molecules closely together in a enzyme-substrate complex, reducing any repulsion between the molecules so that they can be joined more easily.

  2. An Investigation Into the Effect of Substrate Concentration On the Rate of Enzyme Activity.

    The time taken for the bead to rise from the bottom of the test tube to the top was recorded onto the table. This was repeated for each of 40oC test tubes. The results were recorded onto the table. The next temperature was meant to be 50oC, unfortunately a heat block with this temperature was not available.

  1. WHAT EFFECT DOES SUBSTRATE HAVE ON THE RATE OF RESPIRATION IN SACCHAROMYCES CEREVISIAE?

    This temperature Y� is called the optimum temperature. It is the temperature at which the enzyme works best at. After Y�, the rate of reaction falls grinding to a halt. This is because above Y�, the enzyme has denatured and therefore can no longer catalyse any reaction or work on any substrates.

  2. Investigating the Effect of Glucose Concentration On the Rate of Reproduction of Yeast Cells

    The equation for the population is just: dN/dt=rN((K-N)/K) Where r is a constant (the intrinsic rate of increase, i.e. the number of reproductive events per unit time per yeast cell), t the time they have been multiplying for, K the maximum number of individuals that the particular environment can support and N is the yeast population number.

  1. Trypsin. Hypothesis: - I hypothesize that as the temperature increases the rate of enzyme ...

    Delivery tube: - For the passage of oxygen between the test tube containing hydrogen peroxide and potato piece to the water filled measuring cylinder in the trough. 16. Lab coat: - I will be wearing lab coat so that no chemical spills on my clothes.

  2. An Investigation on the Effect of Enzyme Concentration on rate of hydrogen peroxide breakdown.

    Provided that the substrate concentration is maintained at a high level, and other conditions such as pH and temperature are kept constant, the rate of reaction is directly proportional to the enzyme concentration. Suppose that there is an infinite number of substrate molecules, addition of enzymes means that more active

  1. Investigation in to factors affecting the rate of an enzyme catalysed reaction.

    H202 (10vol) 1.5 grams of liver chunks Water Bath Basin Conical Flask Rubber Bung Delivery Tube Measuring Cylinder (x2) Test Tube (x8) Burette Stand Mercury Thermometer (x2) Stopwatch Electric Scales Before starting the main experiment I will carry out

  2. Investigate the effect of enzyme concentration on the rate of reaction.

    Also required to keep the solution in the boiling tubes at a temperature of 37oc - 40oc. Stopwatch 1 To measure the time taken for the enzyme amylase to break down the starch. Kettle 1 To boil the water to be used for the experiment.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work