• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The Effect of Enzyme Concentration On Enzyme Activity

Extracts from this document...

Introduction

THE EFFECT OF ENZYME CONCENTRATION ON ENZYME ACTIVITY AIM: To investigate the effect of a reduction in enzyme concentration on the rate of reaction, in this case the breakdown of protein by protease enzyme. The protein substrate in this reaction will be trypsin milkprotein (casein). PREDICTION: I believe thath a higher enzyme concentration results in protein trypsin to be broken down faster/ a faster rate of reaction. The protein solution would turn clear quicker when enzymes are more concentrated. The site of the reaction occurs in an area on the surface of the protein (protease) called the active site. At low enzyme concentration there is great competition for the active sites and the rate of reaction is low. As the enzyme concentration increases, there are more active sites and the reaction can proceed at a faster rate. Therefore i believe as the protease concentration is reduced, the rate of reaction, as well as the trypsin mlk protein, is also reduced. FACTORS AFFECTING THE RATE OF REACTION: Temperature - enzmes work best at an optimum temperature. Below this, an increase in temperature provides more kinetic energy to the molecules involved. ...read more.

Middle

-glassware for diluting enzyme -milk powder solution -test tube holder -waterproof pen -ruler -standard acidific trypsin solution 19% -5cm3 pipettes 50cm3 beaker -glassware for dilution of the enzyme METHOD: 5cm3 protein solution was pipetted into a test tube and mixed thoroughly. The stop clock was then immediately started and the time taken for the protein solution to clear was recorded. This was repeated two more times for accuracy. These exact procedures were repeated with different enzyme concentrations, ensuring that the conditions are unchanged. The different enzyme concentrations were used: 1% - 5cm3 enzyme solution STRONGER 0.5% - 2.5cm3 and 2.5cm3 water 0.25% - 1.25cm3 enzyme and 3.75cm3 water 0.10% - 0.5cm3 enzyme and 4.5cm3 water WEAKER RESULTS: CONCLUSION: The results i have obtained have shown that a decrease in concentration of enzymes results in an increase in the timetaken for the solution to clear. In other words, as the concentration of protease decreases, the amount of active where the reaction takes place also decrease, therefore causing a lower rate of reaction - the time taken for the trypsin milk protein to be broken down is slower. ...read more.

Conclusion

Here, a flask could be used to mix the two solutions together. When adding the two solutions together, the flask would be inverted a reasonable amount of times, approximately six. The amount of times it is inverted must stay constant throughout each experiment to avoid unfairness. Inverting must be done slowly to allow time of proper mixing between the two solutions, also so the solution can reach both ends of the flask each time inverting. Carrying this process ut can result in a much better chance of a fully mixed standard solution. Other factors in which error could have occured are inaccurate measuring - here, i can only read measurements accurately to the nearest millametre, when to start timing - here, starting the clock must be done immediately after mixing the solutions, and some solutions may have taken longer to mix, and the clock therefore being started at different times during each experiment, and temperature of the surroundings - temperature can affect the rate of reaction. If the temperature of the surroundings changes, this can cause a temperature change in the solution. As stated earlier, enzymes work best at an optimum temperature. Below this, the rate of reaction increases, and above this, the rate of reaction decreases, both causing inaccurate results. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

4 star(s)

This is a well written and structured report.
1. The report needs to have an introduction section that covers relevant background information
2. The report does not include the data that has been collected
3. The conclusion needs to attempt to explain the patterns that have been found
4. The evaluation needs to suggest further research opportunities
****

Marked by teacher Luke Smithen 17/09/2013

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Effects of Copper Sulphate on the Activity of Catalase

    Initial rate of reaction was worked out as follows For example, if I recorded that 20ml of oxygen was produced in 30 seconds, then in one minute (2*30) an estimated 40ml (2*20) of oxygen will be produced. The units of the rate of reaction are therefore in ml/min.

  2. The Effect of Concentration on Pectinase Using Apple

    evaporating away and therefore some sort of seal needed to be put over the test tube. A small sheet of clingfilm will be placed over each test tube. Results of Pilot Experiment: This is a table of the results obtained in the pilot experiment: Conc.

  1. Investigating the effect of temperature on the activity of protease enzyme.

    After this time the two solutions were mixed together and placed back in the water bath for a further 4 minutes, allowing a minute for the result to be taken using the colorimeter. These timings are again based around the preliminary experiment when it took around 5 minutes to see a difference in the solutions at room temperature.

  2. An experiment to show how protease enzymes are affected by temperature

    Then 2 beakers, 3 boiling tubes, 3 test tubes and a thermometer were set up as shown in the diagram on the previous page. Next using a measuring cylinder, each one of the 3 boiling tubes where filled with 30ml of marvel milk.

  1. Type - 1 Hypersensitivity Reaction

    from binding onto the H1 receptors that are located on the surface of the ileum. Suffice to say that, when mepyramine binds onto the H1 receptors the histamine cannot bind on, once the binding onto a receptor on the surface of the (guinea pig ileum)

  2. Investigating The Activity Of The Enzyme Lipase On Milk

    The higher the temperature, the more collisions occur between enzyme and substrate, which means there is more change of an enzyme binding with the correct substrate to complete the Lock and Key Hypothesis more rapidly. When a certain temperature is reached, enzymes will denature and no longer be able to fit substrate molecules into them.

  1. An experiment to investigate the effect of temperature on the action of the enzyme ...

    Everything was fine until we had to add the lipase. I had to somehow manage to put lipase into two test tubes without it going everywhere. This was quite unsuccessful and may have contributed to the anomalous results I obtained.

  2. Investigating how different concentrations of a antibiotic effects the growth of a bacterium.

    it is a very high risk when using as it may react at high temperatures and a number of metals. Therefore there must be a number of precautions taken. These include: o The precaution of the high temperature from the Bunsen and ethanol, so they always been kept away from

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work