The Effect of Enzyme Concentration On Enzyme Activity

The first 200 words of this essay...

THE EFFECT OF ENZYME CONCENTRATION ON ENZYME ACTIVITY

AIM: To investigate the effect of a reduction in enzyme concentration on the rate of reaction, in this case the breakdown of protein by protease enzyme. The protein substrate in this reaction will be trypsin milkprotein (casein).

PREDICTION: I believe thath a higher enzyme concentration results in protein trypsin to be broken down faster/ a faster rate of reaction. The protein solution would turn clear quicker when enzymes are more concentrated.

The site of the reaction occurs in an area on the surface of the protein (protease) called the active site. At low enzyme concentration there is great competition for the active sites and the rate of reaction is low. As the enzyme concentration increases, there are more active sites and the reaction can proceed at a faster rate.

Therefore i believe as the protease concentration is reduced, the rate of reaction, as well as the trypsin mlk protein, is also reduced.

FACTORS AFFECTING THE RATE OF REACTION:

Temperature - enzmes work best at an optimum temperature.

Below this, an increase in temperature provides more kinetic energy to the molecules involved. The numbers of collisions