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The enzyme reaction on milk.

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Introduction

The enzyme reaction on milk AIM: Effect of the temperature on the rate of an enzyme reaction. PREDICTION: I think that higher the temperature will be the quicker the enzyme reaction will take place. I think that if the temperature is between 0-10?C the rate of reaction will be slow because the temperature will cause less collisions and denaturing of enzymes. But if the temperature were between 10-60?C there would be reactions taking place. Particles will be moving fast and the will be colliding with one another and vibrating with each other. But if the temperature is above 60?C I think that the enzyme will de-nature and its molecular structure will change shape and the substrate will not fit into the enzyme active site. This is what I think my graph will look like: A =At this point I think there wont be much of a reaction taking place at a low temperature between 0-10?C. If there is a reaction taking place I think it will take a long time. B= At this point I think there will be a reaction taking place and it will take a short time to break down the milk. I think this will give the molecules heat and activation energy to move into the enzyme active site. There will be collisions taking place too. C= At this point I think the enzymes will de-nature because there will be too much heat and activation energy going to the enzymes and there will be too many collisions and vibrations taking place between the particles. ...read more.

Middle

tube DIAGRAM: METHOD: 1: First I will make a colour standard that I will compare with all of my other test tubes at different temperatures and compare it to see if the milk has been digested. To make the colour standard I will mix 5cm3 of hydrochloric acid with 5cm3 of semi-skimmed milk. The substances should then go translucent. This will show the milk digested. 2: Then I will measure the room temperature and add 5cm3 of trypsin and 5cm3 of semi-skimmed milk together. Then I will mix it together and start the stop clock. Once the mixture has become the same colour as the colour standard I will stop the stop clock and record the time taken for the milk to be digested on to a table. 3: Then I will repeat the experiment a few more times but changing the temperature and mixing the too solutions once they are both at same temperature. Then I will again compare it to my colour standard and record the time taken for the solution to be digested. I will record my results onto a table at first and then plot my results onto a graph to see if there is a pattern or not. I will be changing the temperature and I will be recording the time and stopping it once it has digested. I will need to be accurate for when I'm reading the temperatures off the thermometer. I will use water baths and ice to change the temperatures. ...read more.

Conclusion

There are many possibilities of why an anonymous point occurred. It could have been for various reasons such as whilst I was heating up the solutions in the water bath the window might have been opened and the wind could have altered the temperatures to be different. I might have not stopped the stop clocks at the time of the reaction had taken placed and might have overrun the time. The results were quite accurate as I got a smooth line of best fit and I found a pattern that matched my prediction too. If I were to do this experiment again I would write down the different temperatures that I want the solutions to be at and not have to wait until they are the same temperature because I would save more time like this. Also I would make sure I have a reliable stop clock that will stop when you press the stop button and also I would do the experiment again for about 4 times for accurate results from the stop clock and that the milk has been digested fully and has turned translucent. Finally because I was doing the experiment in a group and we were all over the place we didn't know what results to put down because they were doing it at different temperatures so it was difficult to know what they were saying, so next time I would make sure thay knew what specific temperatures they were going to do so I know what to write down on my results table. KAJAL GONDALIA 10A ...read more.

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