• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The Rate of Decomposition of Hydrogen Peroxide with Catalase.

Extracts from this document...

Introduction

The Rate of Decomposition of Hydrogen Peroxide with Catalase -PLANNING- Introduction Catalase is a biological catalyst or enzyme. It is made up of protein molecules which are found in living cells. Enzymes speed up reactions in the body. Catalase is found in potatoes and liver and is the enzyme used to speed up the decomposition of Hydrogen Peroxide in the human body. Hydrogen Peroxide is also found in the human body however it is toxic to human cells, lipids, DNA and protein. It is a bi-product of metabolism. Examples of the reaction which produce Hydrogen Peroxide are amino acids being converted into fuel and lipids being converted into carbohydrates. Hydrogen Peroxide decomposes naturally, but slowly. The products of this are Water and Oxygen. The chemical formula for Hydrogen Peroxide is H2O2. An enzyme works if it has the same shape active site as that of the substance it is reacting with (substrate). The reaction or decomposition then occurs in the active site and the products leave. Human Enzymes are destroyed above temperature of 37 degrees C by their active sites being altered and no-longer fitting the shape of their substrate. Enzymes are never used up in a reaction so can continue to react with more substrates after one reaction. ...read more.

Middle

Timer - With minutes and seconds - Split seconds cannot be accurately used when forming a conclusion from results. Yeast solution Hydrogen Peroxide Distilled Water Right Armed test tube Diagram Method > Set up the apparatus as in the diagram > Measure out the concentration of yeast solution to 1ml with the 1ml syringe i.e (0.8ml yeast, 0.2ml distilled water for 80%. 0.6ml, 0.4ml distilled water for 60%. Etc.) > Place the 1ml solution into the test tube and then replace the bung. > Measure out 2ml of hydrogen peroxide with the 5ml syringe and place in the bung. > Start the timer when the hydrogen peroxide hits the yeast solution. > When the 20cm3 syringe gets to the 10cm3 mark stop the timer and record the result. > Repeat this for each percentage - 20%, 40%, 60%, 80%, and 100%. > Repeat all of these 3 times. Safety The hydrogen peroxide needs to be treated with care in the experiment as it can bleach skin and clothing. Therefore eye protection should be worn and loose garments taken off. Fair Test/ Accuracy To ensure that it is a fair test the temperature of the water bath will be monitored throughout the experiment if it changes +/- 0.5 then it will be brought back up/down. ...read more.

Conclusion

So with a 100% concentration catalyst - Catalase and a 100% concentration substance - Hydrogen Peroxide, the time taken for the Hydrogen Peroxide catalysing and decomposing is twice the time taken for Hydrogen Peroxide at 100% catalysing with Catalase at 50%; This is because there is another substance involved in the collisions - water. This is shown more clearly in the diagrams below: The conclusion has supported the prediction. As the conclusion shows that as the concentration goes up the rate and time taken for the decomposition of Hydrogen Peroxide will also go up. The conclusion also shows the quantative prediction to be true. That as the concentration of the Catalase doubles the time taken for the decomposition will halve. This is shown by the exponential curve on the graph. Rate has also been shown to be directly proportional to the concentration of Catalase by the straight line on the rate/concentration graph. My prediction was correct because the experiment uses the collision theory and how reaction time is affected by concentration. - Evaluation - The procedure used was adequate in getting the results found although if more time has been given the accuracy of results may have been better. There were a few anomalies found in this experiment Alex Oakes 11Rd Biology Sc1 Coursework Set 11J1 19/11/2002 5:40 PM Page 1 of 9 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. To investigate the rate at which hydrogen peroxide is broken down by the enzyme ...

    10 seconds, until the reaction has been going for a whole minute. This is because I don't have enough time to record the reaction until it stops, and it is also long enough to give a good idea of the rate of reaction.

  2. Investigating The Effect of Substrate Concentration or Temperature On The Rate of Decomposition of ...

    Each cell contains several hundred enzymes, which catalyse a vast number of chemical reactions. Enzymes are known as biological catalysts as they significantly increase the rate at which reactions occur within living organisms, without being used-up or affecting the reaction in any other way.

  1. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    things, animals and in plants shows that water is unlikely to damage the catalase molecules. Water is also highly un-reactive - it will not act as an oxidising agent (it won't bond with or take any electrons from the catalase molecule).

  2. Reaction of Catalase and Hydrogen Peroxide

    For defence against H2O2 cells contain Catalase. Each type pf organism has its own Catalase, which probably differs at least slightly in other species of organism. The rate of which enzymes catalyse reaction varies with their environment. Enzymes work best within an optimum range of physical and chemical conditions.

  1. How do temperature and concentration affect the rate of decomposition of hydrogen peroxide by ...

    product of metabolism does break down naturally but for this experiment I am going to use a catalyst to speed the reaction up. The presence of this catalyst lowers the activation enthalpy by weakening the bonds present in the substrate so less energy is required to break the bonds and form new products.

  2. Experiment to Investigate a Factor which Affects the Rate of Decomposition of Hydrogen Peroxide ...

    As temperature continues to increase, so does the speed of the molecules. However, at a certain temperature, being slightly different for each enzyme, the structure of the enzyme molecule starts vibrating too violently for the bonds holding the molecule together, so some of the bonds begin to break, especially the hydrogen bonds.

  1. Investigating the break down of Hydrogen Peroxide using catalyst

    This variable involves the collision theory, which says that higher temperature particles have more energy. This energy (kinetic) enables the particles to move around at a faster speed. If the particles are moving around faster, then they are more likely to collide frequently.

  2. Plant Metabolism.

    The activity of SBPase is regulated by a number of different factors; in the light SBPase activity is increased 12-fold by thioredoxin f and is further modulated by pH and Mg2+. A finer level of control is exerted by the products of the SBPase reaction, SBP and Pi and also by glycerate.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work