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The use of pectinase in fruit juice production

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Introduction

The use of pectinase in fruit juice production I predict that there will be no juice produced to the apple sauce that had no enzyme and juice will produce to the apple sauce that had pectinase. A control is carried out in order to compare the rate of reaction between with pectinase and without pectin's. Pectin --> sugars + galataronic acid Pectin is a substance which helps to hold pant cell walls together. As a fruit ripens the plant produces proteolytic enzymes, which convert the insoluble protpectin of the unripe fruit into more soluble forms, causing the fruit to soften. When fruits are mashed and pressed to form juices these more soluble forms of pectin enter the juice, making it cloudy and causing the colour and flavour to deteriorate. Enzymes are specific in the reactions they catalyse, much more so than inorganic Catalysts. Normally, a given enzyme will Catalyse only one reaction, or type of reaction. The enzyme has an active site that helps it to recognise its substrate in a very specific way. Just like a key only fits into a specific lock, each enzyme has its own specific lock; each enzyme has its own specific substrate. This is called the lock and key theory. The enzymes never actually get consumed in the process; they just increase the rate of reactions. ...read more.

Middle

3. Mix carefully and leave to stand for 5 minutes. 4. Transfer the contents of the beakers to separate filter funnels and collect the juice produced in a graduated measuring cylinder. 5. Take reading of the juice collected. Results Table to shows apple juice in cm3 collected over time in minutes Time in min Volume in cm3 Pectinase Highest Lowest Average No Pectinase 0 0.00 0.00 0.00 0.00 0.00 0.00 1 0.50 1.00 1.00 0.50 0.75 0.00 2 1.00 1.50 1.50 1.00 1.25 0.00 3 1.50 2.00 2.00 1.50 1.75 0.00 4 2.00 2.25 2.25 2.00 2.13 0.00 5 2.50 2.50 2.50 2.50 2.50 0.00 6 2.75 2.75 2.75 2.75 2.75 0.00 7 3.00 3.00 3.00 3.00 3.00 0.00 8 3.25 3.00 3.25 3.00 3.13 0.00 Table to shows initial rate of the enzyme pectinase when breaking down pectin into sugars and galataronic acid Time in min Initial rate cm3 min-1 0 0 1 0.75 2 0.625 3 0.58 4 0.53 5 0.50 6 0.46 7 0.43 8 0.39 Analysis The hypothesis is proved that the break down of pectin with pectinase was successful and there was no reaction with the apple sauce that exclusive of pectinase. The pectinase causes disulphide bonds to break. These bonds help to maintain the shape of the enzyme molecules. ...read more.

Conclusion

After that, there was a gradually decrease of roughly 0.05 cm3. A graph showing the rate of reaction of the enzyme pectinase when breaking down pectin into sugars and galataronic acid is also drawn onto the back of the graph which shows the volume of apple juice in cm3 over time in minutes. Error bars provide a simple and clear way to represent the uncertainty of each point on a graph. Error bars are drawn onto the graph, which shows the volume of apple juice in cm3 over time in minutes. There were big error bars from minute 1 to minute 4 intervals ; they all have a range of 0.5cm3 within the average. Then the error bar was minimised to within a narrower range of 0.25cm3 at 4th and 8th minute intervals. There was no error bar at 5th, 6th and 7th minute intervals, which indicates that the experiment results were agreed by both of the groups. The bigger the error bars, indicates the more inaccurate of the results that were collated hence the results were unreliable and which would effect our prediction of the outcome of the experiment. Evaluation There were insufficient sets of results in order to make this experiment reliable since there were only two sets of results. To improve the accuracy of this experiment, repeats should be done. The apple sauce that was prepared had a big impact on the experiment. ...read more.

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A full account of the investigation. Occasionally a lack of clarification or detail reduces the quality.

Marked by teacher Adam Roberts 29/05/2013

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