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The Vitamin C content of fruit juice.

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Introduction

The Vitamin C content of fruit juice. James Banham 11/8/2008 Which type of fruit juices provides the most vitamin C? In this experiment, I will be trying to answer the question 'Which type of fruit juices provides the most vitamin C?'. To achieve this I will test the vitamin C content in four different types of juices using Dichlorophenol-indophenol (DCPIP). From the results I obtain through the experiment I will be able to see a clear outcome of which of the fruit juices contain the most Vitamin C and which contain the least. Vitamin C is ascorbic acid: The presence of ascorbate is essential for a range of important metabolic reactions in all animals and plants. It is made internally by more or less all organisms, humans being the most renowned exception. It is extensively known as the vitamin whose deficiency causes scurvy in humans. It is also widely used as a food additive. ...read more.

Middle

I will now repeat this using the fruit juices and record how many millilitres of vitamin C used to change the colour. Results (DCPIP) 1) 9ml of vitamin C, before it went clear the final measurement was 8.4ml - 0.6ml used to change colour 2) 9ml of vitamin C, before it went clear the final measurement was 8.3ml - 0.7ml used to change colour 3) 9ml of vitamin C, before it went clear the final measurement was 8.9ml - 0.1ml used to change colour (this may have happened because the test tube was not cleared out properly or the DCPIP used was an older batch) 4) 9ml of vitamin C, before it went clear the final measurement was 8.6ml - 0.4ml used to change colour 5) 9ml of vitamin C, before it went clear the final measurement was 8.6ml - 0.4ml used to change colour Results (fruit Juice) Apple Juice (100% from concentrate) ...read more.

Conclusion

Orange 50 Pineapple 30 Grapefruit 10 Apple 6 From the averages here I can clearly see that orange provides the most Vitamin C, 7 times more than an average apple. However there were twenty-four other fruits and vegetable that contained considerably more than orange, surprisingly broccoli was one of them containing 90mg per 100g. Comparing my results with the ones in the table I can see that I have successfully proved that the orange juice has the highest vitamin C content. Also from my results I can see pineapple juice is the second highest in concentration and that the apple juice provides the least. My results are identical with the information in the table. I can now also tell that I correctly performed the experiment and proved my hypothesis correct. Conclusion From the question I was given at the beginning, I have found out that the hypothesis I drew up was accurate. The orange juice provided 30mg3cm vitamin C content whereas the apple provided only 16.2mg3cm. I believe that using the DCPIP on the individual juices three times was exceptionally helpful as it gave me reasonably accurate results. ...read more.

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