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To investigate the effect of temperature on the rate of reaction on trypsin enzymes.

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Aim: To investigate the effect of temperature on the rate of reaction on trypsin enzymes. Introduction: I predict that the best temperature for the reaction to take place will be at around 40 degrees. I made this assumption on the basis that 40 degrees is the closest to body temperature, and so this would have to be the best temperature for the reaction to take place. It can also be said that below 40 degrees the enzymes will have less energy and hence will move around less. Therefore there is less chance that they will collide into the photographic film, meaning the time that it takes for the enzyme to fully react with the photographic film will take longer. Also above 40� the enzyme will be affected by the high temperatures and will begin to denature. When an enzyme becomes denatured its active site changes shape and so it cannot break down any substances. Therefore at above 40� enzymes will be denatured, unable to break down the photographic film, and so the reaction will take longer. The results from my preliminary experiment have shown me that the acclimatisation time should be 4 minutes, and that an end point of 10 minutes is enough time for us to be able to tell that no reaction will take place. ...read more.


5. Repeat for other test tubes. 6. Cut a notch on the end of one side of all the splints. 7. Carefully place a 1cm� piece of photographic film in each notch on the splints. 8. Place three test tubes in the water bath. 9. Set the thermostat on the water bath to 10�C. 10. Wait 10-12 minutes for the water to settle at 10�C. 11. Place a splint with photographic film into each of the test tubes. 12. Start timing using the stopwatch. 13. Wait for the film to dissolve away. 14. When the film has eventually dissolved away, stop the time and note it down. 15. Clean the test tubes and apparatus thoroughly. 16. Repeat this process from step 1 to 15 for temperatures 20�C, 40�C, 60�C and 80�C. Diagram: Fair test: To ensure the experiment is a fair test: * Ensure the same amount of trypsin in used throughout the experiment. * Ensure the same size of photographic film is used throughout the experiment. * Carry out the experiment three times to ensure the results are valid and are reasonable. * Ensure the trypsin is acclimatised before the experiment. ...read more.


Although there are so many possible in accuracies that may have occurred, my main hypotheses was proven correct, As we have the exact same curve in the final experiment as predicted in my plan. In terms of accuracy, the results are reliable enough to depend on to conclude whether the predictions were correct or not. The anomalies were not too extreme and thus can still be considered in taking into account as a result. I could have made many improvements during the experiment to make it fair and equal. An example of this is the range of temperature; if the experiment was carried out at 10�C intervals, there would have been a more defined and accurate graph. Conclusion: By looking at my results I can conclude that the optimum temperature is near the average body temperature; 37�C. This is because the enzymes are designed to work in the body where the temperature is around 37�C. We can also tell from the graph that the enzymes stop working at low temperatures, and denature at high temperatures. There were no real noticeable anomalies that can be noted as outside the conventional result and thus there were no results outside the expected results. GCSE Biology Coursework Amir Ashrafi ...read more.

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