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An Investigation Studying the Effect Catalse on Different Temperatures of Hydrogen Peroxide.

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An Investigation Studying the Effect Catalse on Different Temperatures of Hydrogen Peroxide Aim: Investigating the affect of different temperatures of hydrogen peroxide on Catalse. Background Theory: Enzymes are chemicals that help increase the rate of reaction of the chemical reactions that take place in cells of living things. We need these reactions to happen quickly to keep us alive. Enzymes make reactions happen at a much faster rate. Chemicals that speed up reactions are called catalysts, and since enzymes speed up chemical reactions in our body, they are called biological catalysts. Enzymes are put into two different groups, Breakers and Builders. 1. Breakers: Large molecules need to be broken down into smaller ones, which is important for the digestion when the smaller ones, which is important for digestion when the smaller ones, which is important for digestion when the smaller ones could be used. Breaker enzymes speed up these reactions. The substrate is the substance the enzyme works on, when the reaction takes place this is called the active site. The product released is the broken-down substrate, due to reaction with the enzyme. 2. Builders: In other reactions large molecules are needed to be formed by small ones, builders enzymes speed up building these molecules from smaller ones which are important molecules inside cells. In Builder enzymes the product released is the built up substrate that are resulted by reaction of the substrate with the enzyme. ...read more.


Method: * Set apparatus ready. * Cut small pieces of potato weighting 2 grams each (make sure that you cut all the pieces from the same part of the potato, because some parts of the potato tend to have more Catalse than other parts.) * To check that all the pieces weigh 2 grams, put each piece on the electronic scale. * Crush each piece in the crushing dish; make sure all crushed pieces are kept separate from the other pieces. * Measure 20 ml of hydrogen peroxide in the measuring cylinder (make sure the measuring cylinder is at your eye level to be accurate). * Get stop watch ready * Mix the crushed potatoes with the hydrogen peroxide in the measuring cylinder. * Press on the start button as soon as the potatoes touch the hydrogen peroxide, you have to be accurate. * Leave the reaction going for 60 seconds. * Take the measure of the new reading shown on the measuring cylinder right after you stop the timing at 60 seconds. * Subtract the measure you took from 20 ml (Which is the amount of hydrogen peroxide we started with). To get the volume of the reacted bubbles. * Record the results. * Throw out the chemical and wash the apparatus carefully. * Do this for all the temperatures of the hydrogen peroxide. * Repeat the experiment till you are satisfied with its accuracy. ...read more.


which I think were sufficient. I used accurate measuring facilities. E.g. an electronic scale that measured to the nearest 0.01 grams. a measuring cylinder that measured to the nearest 1 ml, which helped me to have no problem with accuracy. I had a reliable method, which helped me to obtain accurate results. I think I had reliable results, which my graphs show. My results were all similar in the different sets of results and graphs. The problem I had with measuring bubbles after the reaction was that it was very hard to measure bubbles were some used to collapse, the bubbles didn't have a constant volume were some were very small and some were big. I could improve the experiment by using a bigger range of temperatures so I can compare my results more accurately and discuss any faults I had. Or increasing the time the reaction is left for, to see if the reaction is going to stop after a certain period of time. Or changing the amount of potato added to the hydrogen peroxide. Or changing the amount of hydrogen peroxide added to the potato to see if this is going to affect the reaction. I could even stop using the temperature of the hydrogen peroxide as a variable and start a new experiment using the P.H. of the hydrogen peroxide as a variable. One thing I could do is changing the dependant variable, which is the height of the bubbles produced after the reaction because it's very hard to measure as I mentioned before. ...read more.

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