An Investigation To Show How Light Intensity Effects The Rate Of Photosynthesis Using The Plant Elodea.

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David Anthony

An Investigation To Show How Light Intensity Effects The Rate Of Photosynthesis Using The Plant Elodea.

Background Information;

In order for the results to show an effect on the changing light intensity I need to know the variables. The other variables will be controlled and become constant so the only factor I will be testing will be light intensity. I will try to do the experiments in the same conditions each time but these will vary. To control this slight variation in room temperature and other factors I will have a range, and use method so stop these hard to control factors from effecting the outcome.

 The products of photosynthesis that I will be using as the outcome and taking results will be the release in oxygen, this will be shown by bubbles released the pond weed will be covered in water as this is it natural environment.  

Variables;

Carbon dioxide Concentration – the water will be taken from a sterile source of distilled water. If this was a factor I was changing I could add a solution containing a source of carbon dioxide to the water changing the ratio each time to see the effect.

Wavelength – this would be testing the wavelength of different coloured light. This could be used to see which is the most efficient and which is not. This would be done by shining a light with a colour filter again seeing the effect on rate of production of oxygen.

Water level – the Elodea is a pond weed it lives under water, because of this the planet will be totally covered submerged too keep with normal environment conditions. This would not be a very good variable to change unless you had a plant that was above ground level and then submerged or a submerged plant and give it less water.

Light intensity - this will be kept constant if it was not the variable, but as it is it will be changed to see how the effect on producing oxygen, showing by bubbles in the water, the rate of photosynthesis in the plant.

Temperature - the higher the temperature the plant up to around 40°C the faster the rate of photosynthesis, this was shown in the pre-test which we carried out. If the temperature increases above this the plant will start to die, as the enzymes in it will denature changing shape and not working.  This will be explained and controlled carefully to insure this does not happen and make anomalous results. 

Equations;

6CO  + 6H0   >>>>>>>> C H0  +  6O

Raw materials                          Products

Outline Plan;

  • The variable I will be changing is “Light intensity on Elodea.”
  • I will find the distance and temperature best suited for the plant in a pre-test.
  • I prepare the equipment setting out in the same way and using the same. Checking that everything is in place and that we keep to the plan so every test is the same.
  • Making use the test tube that the Elodea is kept in a beaker so it will not roll around and be a safety hazard.
  • Take results moving the length and repeating evaluate data collect any more data if there is any anomalous and needs checking out.
  • Pack up equipment.
  • Analysis and the resulting data conclusion including graphs.
  • Evaluating data - showing strengths and weaknesses of the plan and data collected also detailed explanations for the results collected and fault with the plan which made have been made.

Plan;

To calculate the data, collected, estimating which light intensity will be the best for conditions of photosynthesis. Proving this with the use of graphs and averages making my results more creditable. I will be doing the experiment that will not be able to be finished in one day, it will be done over a period of time. This may effect the data collected as weather and other factors come into play, but I hope that this change will be so slight it will not change the general direction that the experiment is following.

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  • Step one – collect Elodea and place in test tube, attached to a paperclip to be weighed down.
  • Step two – cut the top of the plant to make a clean break and to let the bubbles of oxygen bubble out of the hole.
  • Step three - set up the beaker and get the water to the working temperature, making sure all the equipment and table of results are correct.
  • Step four - make sure the lamp is away from any water and there is no spillages.
  • Step five- switch on the lamp.
  • Step six- results ...

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