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biology data analysis

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In this investigation i plan to explore the effects of substrate concentration has on the rate of reaction of the enzyme catalase. I chose to investigate this topic because it will be interesting for me to know how catalase rids the body of toxic substances like hydrogen peroxide by turning it into useful substances like oxygen and water. Background information Catalase is an enzyme found in foods such as potato and liver. It is used for removing Hydrogen Peroxide from the cells. Hydrogen Peroxide is the poisonous by product of metabolism. Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen. The enzyme catalase is made from protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. Each enzyme has very particular role; for example the enzyme catalase rids the body of hydrogen peroxide only. Substrate concentration and the active site of an enzyme have a lock and key relationship. Substrate concentration being the key and an enzymes active site being the lock. When the amount of substrate is too high the substrate will not fit the enzymes active sites. ...read more.


When recording the time it takes for the filter paper to rise to the surface of the hydrogen peroxide the specimen tube should be looked at from an angle of 90 degrees to it to avoid any parallax error. Predictions I predict that there will be a positive correlation between the concentration of hydrogen peroxide and the rate of reaction of catalase. Concentration of Hydrogen Peroxide 100% H2O2: 10ml H20:0ml 80% H2O2:8ml H20:2ml 60% H2O2:6ml H20:4ml 40% H2O2:4ml H20:6ml 20% H2O2:ml H20:8ml Time taken (trial 1) 7.73 10.77 11.12 14.64 25.26 Time taken (trial 2) 6.23 7.78 9.23 11.73 28.31 Time taken (trial 3) 8.03 9.41 10.23 10.10 19.6 Time taken (trial 4) 7.99 9.45 12.41 8.24 19.58 Average 7.5 9.35 10.75 11.18 23.19 Rate of Reaction Results Analysis From looking at the graph i can see that there is a positive correlation between the rate of reaction of catalase and the concentration of substrate in this case this is hydrogen peroxide. When the concentration of Hydrogen Peroxide is increased, the rate of reaction also increases at a directly proportional rate from 20% until 80% concentration of Hydrogen Peroxide. When the concentration is doubled from 20% to 80% the rate of reaction goes up 0.04 to 0.11. ...read more.


b) There is also no way of ensuring that all volumes of Hydrogen Peroxide are the same. c) There is also no way of ensuring temperatures, exposure to light and many other factors remain the same although i doubt whether this would make a great difference in the results it is still possible that this difference could have a profound effect and therefore skew the data. In order to improve the reliability of this investigation i could carry out more trials this would preventing outliers from skewing the data. I could have also used a greater number of concentrations of hydrogen peroxide this would have improved the reliability of the line of best fit may have shown me what the maximum possible rate of reaction was before it began to slow. It would have also have been possible for me to have another person involved in my experiment whose job it would be to start and stop the stop clock. This would have meant a better reaction time and therefore more reliable results. Alternatively we could have deducted 0.1 seconds from the time calculated. This 0.1 seconds would represent the delay in amount of time it took us to start and stop the stop-clock. ?? ?? ?? ?? ...read more.

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