Step 3
I will devise a results table to record the results in. I will weigh each set of discs and then I will record the masses of each group in the results table.
Step 4
I will put each group of discs into each one of the Petri dishes and locate them in a safe area to prevent accidents happening, and leave there for a period of no less than 24 hours.
Step 5
After the 24 hour period is over, I will carefully take out each set of discs from each of the 7 Petri dishes. I will remove any excess liquid from the discs using a filter paper. I have to make sure that I use the same procedure for all the discs in order to make a fir test.
Step 6
I will devise another results table and then record the final results. I will also graph the results by plotting the percentage change in mass against the concentration of the sucrose solution.
Safety instructions
During this investigation no major risks are involved, that could cause serious health injuries. However there are present some minor risks that could affect the outcome of the results, if ignored.
Risks must never be ignored whether serious or not so serious, they have to be carefully analysed, in order to help perform a safe successful investigation.
Based on the experience I have had so far
- I will follow the instructions given to me at the beginning of the experiment given by the teacher.
- Since this investigation will be performed individually I will avoid contact with all students in the class. If I’m stuck and I need help I will ask the teacher to aid me if necessary.
- To reduce the disturbance, and the chances of the equipment being broken I will have to find my own space in the classroom.
Risk assessment
The greatest risk and the most serious of all during this investigation is while slicing the apple. The blade should be carefully handled while cutting the slices because if careless you can easily slice through your fingers and cut any of arteries which can lead to blood loss.
Results
Lipase
Lipases are produced by the pancreas and it acts in the duodenum. The major lipase in children and adults is pancreatic triglyceride lipase (PTL), which requires colipase for activity. They are used for the breakdown of lipids (fatty acids) in our digestive system.
Lipase hydrolyses emulsified triglycerides of the long chain fatty acids. Lipase is active at the interface between the oil drops and the aqueous phase. In normal serum the concentration of lipase is low. In acute pancreatitis and in pancreatic carcinoma a rise in serum lipase activity occurs, with a mean increase being 50 times that of normal values.
Glycerine, is a commercial product of fat and oil processing, which is used in numerous applications because it is non-toxic and has no negative effect on the environment. It is commonly used as a natural sweetener, a humectant (a substance added to another to help retain moisture) a plasticizer (a substance added to plastic to keep it soft and viscous), and a hypoallergenic emollient for cosmetic and personal care products
Industrial uses
Lipases are extensively used in the dairy industry for the hydrolysis of milk fat. It is also used for the flavouring of cheese e.g. Italian cheese
Lipases can be used in the Laundry detergents and automatic dish washing machines detergents. Lipase Enzymes can reduce the environmental load of detergent products, since they save energy by enabling a lower wash temperature to be used.
They are many other industrial uses of the lipase enzyme such as glycerine.
Glycerine is a commercial product of fat and oil processing, which is used in numerous applications because it is non-toxic and has no negative effect on the environment. It is commonly used as a natural sweetener, a humectant (a substance added to another to help retain moisture) a plasticizer (a substance added to plastic to keep it soft and viscous), and a hypoallergenic emollient for cosmetic and personal care products
