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# Determining the water potential of an apple.

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Introduction

As Biology coursework-Unit 2 Determining the water potential of an apple Equipment list Equipment Reason Sucrose solution Because it is the solution in which the apple is tested in Apple It is the 25cm3 measuring cylinder To measure 25cm3 accurately 10cm3 measuring cylinder To measure smaller substances accurately e.g. you need a volume of 30cm3, but all you got to is 27cm3, then you use the 10cm3 measuring cylinder to add another 3cm3. Cork borer To bore through the apple to get the cylinders. Razor blade To cut the apple cylinders into smaller discs. Stirring rod It is used to shove out the apple cylinders from the cork borer. Measuring scale For measuring the masses of the, apple, discs Filter paper To get rid of the excess fluid from the discs. Distilled water It is used for dilutions Tweezers For carrying the discs to prevent contamination. 7 Petri dishes To fill with sucrose solution and then place discs inside. Sticky labels For labelling the Petri dishes in order to prevent confusion. Ruler To measure the length of the discs accurately as all discs must have the same e length. Procedure Step 1 I will choose an appropriate range of the concentrations for the investigation. And then I will label 7 Petri dishes: pure water, 1.00M, 0.5M, 0.75M, 0.5M, 0.4M, 0.3M, 0.2M, and 0.1M. ...read more.

Middle

However there are present some minor risks that could affect the outcome of the results, if ignored. Risks must never be ignored whether serious or not so serious, they have to be carefully analysed, in order to help perform a safe successful investigation. Based on the experience I have had so far * I will follow the instructions given to me at the beginning of the experiment given by the teacher. * Since this investigation will be performed individually I will avoid contact with all students in the class. If I'm stuck and I need help I will ask the teacher to aid me if necessary. * To reduce the disturbance, and the chances of the equipment being broken I will have to find my own space in the classroom. Risk assessment What could happen? What would I do if this happened? How could I prevent this from happening? *Slice through my finger while cutting the apple. I would immediately alert the teacher and seek medical attention. Carefully handle the razor blade and cut slowly. Could drop and break equipment such as test tubes I would clean up the mess using two pieces of paper to collect the broken pieces or use a sweeper to sweep. ...read more.

Conclusion

In acute pancreatitis and in pancreatic carcinoma a rise in serum lipase activity occurs, with a mean increase being 50 times that of normal values. Glycerine, is a commercial product of fat and oil processing, which is used in numerous applications because it is non-toxic and has no negative effect on the environment. It is commonly used as a natural sweetener, a humectant (a substance added to another to help retain moisture) a plasticizer (a substance added to plastic to keep it soft and viscous), and a hypoallergenic emollient for cosmetic and personal care products Industrial uses Lipases are extensively used in the dairy industry for the hydrolysis of milk fat. It is also used for the flavouring of cheese e.g. Italian cheese Lipases can be used in the Laundry detergents and automatic dish washing machines detergents. Lipase Enzymes can reduce the environmental load of detergent products, since they save energy by enabling a lower wash temperature to be used. They are many other industrial uses of the lipase enzyme such as glycerine. Glycerine is a commercial product of fat and oil processing, which is used in numerous applications because it is non-toxic and has no negative effect on the environment. It is commonly used as a natural sweetener, a humectant (a substance added to another to help retain moisture) a plasticizer (a substance added to plastic to keep it soft and viscous), and a hypoallergenic emollient for cosmetic and personal care products ...read more.

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