Does the Volume of Amylase Affect the Rate of Reaction between Amylase and Starch?
Plan
Aim:
The aim of this investigation is to find out whether the volume of amylase affects the rate of reaction between amylase and starch.
Prediction:
I predict that the greater the volume of amylase then the faster the rate of reaction between the starch and amylase will be. I predict this because of the lock and key hypothesis. The lock and key hypothesis explains how the substrate molecule (the starch) fits inside the enzyme. The substrate molecule is then broken up into many smaller pieces.
In this experiment the larger the volume of amylase (enzyme/active site) there is then the quicker the starch (substrate) will be broken down, resulting in a fast reaction rate. Therefore, a smaller amount of amylase will result in a slower reaction rate.
Preliminary Work:
The preliminary work was carried out to find a suitable range to collect data in. From the preliminary work I was able to determine suitable intervals of time to collect data. It showed that a volume of amylase below 4cm³ took a very long time to react thus making the experiment take too long. From this I worked out that I should start at a volume of 4cm³. The preliminary work also proved to me that my basic method worked without any set backs that may affect my results.
Variables:
The variables involved in the rate of reaction between amylase and starch are:
The volume of amylase
The volume of starch
The temperature of amylase
The temperature of starch
Room temperature
Concentration
Plan
Aim:
The aim of this investigation is to find out whether the volume of amylase affects the rate of reaction between amylase and starch.
Prediction:
I predict that the greater the volume of amylase then the faster the rate of reaction between the starch and amylase will be. I predict this because of the lock and key hypothesis. The lock and key hypothesis explains how the substrate molecule (the starch) fits inside the enzyme. The substrate molecule is then broken up into many smaller pieces.
In this experiment the larger the volume of amylase (enzyme/active site) there is then the quicker the starch (substrate) will be broken down, resulting in a fast reaction rate. Therefore, a smaller amount of amylase will result in a slower reaction rate.
Preliminary Work:
The preliminary work was carried out to find a suitable range to collect data in. From the preliminary work I was able to determine suitable intervals of time to collect data. It showed that a volume of amylase below 4cm³ took a very long time to react thus making the experiment take too long. From this I worked out that I should start at a volume of 4cm³. The preliminary work also proved to me that my basic method worked without any set backs that may affect my results.
Variables:
The variables involved in the rate of reaction between amylase and starch are:
The volume of amylase
The volume of starch
The temperature of amylase
The temperature of starch
Room temperature
Concentration