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Does the Volume of Amylase Affect the Rate of Reaction between Amylase and Starch?

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Introduction

Does the Volume of Amylase Affect the Rate of Reaction between Amylase and Starch? Plan Aim: The aim of this investigation is to find out whether the volume of amylase affects the rate of reaction between amylase and starch. Prediction: I predict that the greater the volume of amylase then the faster the rate of reaction between the starch and amylase will be. I predict this because of the lock and key hypothesis. The lock and key hypothesis explains how the substrate molecule (the starch) fits inside the enzyme. The substrate molecule is then broken up into many smaller pieces. In this experiment the larger the volume of amylase (enzyme/active site) there is then the quicker the starch (substrate) will be broken down, resulting in a fast reaction rate. Therefore, a smaller amount of amylase will result in a slower reaction rate. Preliminary Work: The preliminary work was carried out to find a suitable range to collect data in. From the preliminary work I was able to determine suitable intervals of time to collect data. It showed that a volume of amylase below 4cm� took a very long time to react thus making the experiment take too long. From this I worked out that I should start at a volume of 4cm�. ...read more.

Middle

The colorimeter is relevant as it will allow me to show clearly and accurately the rates of reaction of the different volumes of amylase. I enables me to do this by telling me the percentage light absorption of the different solutions which can then be compared on a graph. Method: Make sure that the colorimeter is set up and that is has been calibrated. Insert Filter 470 into the filter slot. Put 0.1cm� of starch in a test tube. Add 0.01cm� of iodine solution to the test tube. The contents will go blue/black. Add 4.0cm� of amylase to the test tube. Immediately pour it into to a cuvett and place the cuvett into the colorimeter. Measure the reading on the colorimeter 11 times, once as soon as it is placed in the colorimeter and the following 10 times at intervals of 30 seconds. Do this 6 times, increasing the volume of amylase by 1.0cm� each time. The following volumes of amylase will be tested; 4.0cm� 5.0cm� 6.0cm� 7.0cm� 8.0cm� 9.0cm� Repeat each volume of amylase 2 times and then work out an average. Observation Results Collected: Analysis Percentage Light Transmission Averages: Rate of Reactions: From my graphs it is visible that as the volume of amylase is increased the rate of reaction between the starch and amylase does not. ...read more.

Conclusion

The use of the colorimeter really helped and aided me in making the experiment as accurate as possible. Another thing that benefited the accuracy of the experiment was the colorimeter we used. There were two different types; one which gave an analogue reading and one which gave a digital reading. I managed to use the one which gave a digital reading which significantly reduced the risk of human error because if I had used the one with the analogue reading I may have read the dial wrong. The reason I think that the second test of 9.0cm� of amylase went wrong is because I performed the experiment on different days. This could have meant that the room temperature was different. This could have affected the experiments results because enzymes work better when the temperature is hotter thus if the temperatures were different on the two days the experiment was carried out then the results will be different. This brings me on to the time aspect. I think if I had had more time and was able to do my experiment on one day then my results may have been better as the temperature would have been the same. I think overall the experiment went ok. Although I wasn't able to prove my prediction correct I feel that it is unproven rather than disproven. I fell that my method could be improved dramatically as stated above. Biology TCW December 07 Biology Coursework 1/7 Tim Wroblewski ...read more.

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