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Effect of Temperature on Rate of Reaction of catalase

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Effect of Temperature on Rate of Reaction of catalase Introduction This investigation was in an attempt to try to find out how varying the temperature can affect an enzyme. The enzyme used was catalase which breaks down Hydrogen Peroxide, this gives off water and oxygen as effervescence. This effervescence is what is used to measure the reaction rate of the catalase. The optimal heat for enzyme activity is proven to be 37oC as anything above this denatures the enzyme. Denaturing is where the heat energy of breaks down the di-sulphide, ionic and hydrogen bonds that hold the tertiary structure together, this in turn changes the shape of the active site and so halts any reactions that the enzyme would otherwise be able to catalyze. Method 6 test tubes were set up and 2cm squared of Hydrogen Peroxide was placed in each tube. ...read more.


Discussion As the temperature rose the particles began to vibrate faster, this is turn caused more collisions between particles. This is why the reaction rate rose with the temperature, however after a certain point (40oC), the enzymes were becoming denatured and this caused them to become useless which is why the reaction slowed to a halt after this temperature. This was a poor experiment because: The potato chips were very hard to get exactly the same. This inaccuracy caused the surface area to differ between chips, which means that a small linear error will make a big surface area error. To counter this, a machine should be used to cut the chips to a preset size and would be very accurate. Measuring effervescence was very inaccurate. The Bubbles tended to burst when they were made which caused the overall amount of bubbles to be inaccurate. ...read more.


Also worth noting in relevance to the pre-mentioned error is the fact that the chip would float under high temperatures which means that the surface area would not be equal all the way through the experiment. A small surface area error would probably lead to a large rate of reaction mis-calculation. The reaction itself caused heat because it is an exothermic reaction which could alter the rate of reaction in itself. During the reaction substrate was used up which caused the reaction to slow to a halt after a while despite the fact that the enzymes were still functional, more substrate than was needed would have countered this and given true readings. Perhaps the most serious error in the whole experiment though was the fact that the gas expanded as the temperatures rose which would throw the results off completely. In order to counter this, the gas would need to be collected and cooled to room temperature in order to get perfectly accurately results. Ashley Cottrell 10th November, 2002 ...read more.

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