InVESTIGATING ENZYMES

Aim 

To find out how enzymes are affected by temperature.

Method

. Mix starch and amylase together to get a solution

. Place it in 6 Beakers

. Place this mixture in 6 Electric baths at different temperatures.

 E.g. 20,25,30,35,40,and 45

. Use a pipette to put 2 drops of the solution at 20oC in a spotting tile

. Then add two drops of Iodine (to test if starch is present-the solution will  

  go black)

. Start a stopwatch and start adding the drops

. Repeat for all the temperatures. Repeat the entire experiment.

. Find the average

. Record the results

Equipment

. Six beakers

. Pipette

. Starch solution

. Iodine

. Amylase Solution

. Thermometer

. White spotting Tiles

. 6 Electric Water baths at 37oC (normal body temperature)

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. A stopwatch

Variables that might affect my results: that might affect my results:

. Unequal amounts of both solutions

. Wrong temperatures

. If my timing is not accurate

. Control?

Fair Test: I will make this a fair test by...

. Adding an equal amount of Iodine and amylase

. Having the right temperature at different times

. Making sure the temperature stays that way

Prediction:

My prediction is that I will able to see how enzymes can be affected by temperature, as when I start adding the drops, the ...

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