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Examine how the concentration of the substrate Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase.

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Introduction

Aims This is an experiment to examine how the concentration of the substrate Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase. Background Information Enzymes such as Catalase are protein molecules, which are found in living cells. They are used to speed up specific reaction within the cell. They are all very specific as each enzyme just performs one particular reaction. Catalase is an enzyme found in food such as potato and liver. It is used for removing Hydrogen Peroxide from cells. Hydrogen Peroxide is the poisonous by-product of metabolism. Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen as shown in the equations below. Apparatus Need For The Experiment. 250 cm3 Glass Beakers, Glass Thistle Funnel, Graduated Measuring Cylinder, .Cork Borer,250 cm3 Glass Cylinders , Digital Stop clock, Scalpel, Tap , Distilled Water, Plastic rule, Safety Goggles. Method To test out how the concentration of Hydrogen Peroxide affects the rate of reaction first set up the apparatus and prepare the different concentrations of Hydrogen peroxide as below: Concentration of Hydrogen Peroxide Volume of Hydrogen Peroxide (cm3) ...read more.

Middle

This gives the rate in cm3 of oxygen produced in the time of 5 minutes, this is because I am timing how much oxygen gas is given off in the space of 5 minutes for the various concentrations of Hydrogen Peroxide. From these results, a graph can be plotted with concentration on the x-axis and the volume of gas released on the y-axis. To ensure this experiment is completed as fairly as possible, all the variables except for the concentration of Hydrogen Peroxide must be kept the same for all experiments. Variables that must not be altered include: Temperature, Catalase Concentration, dimensions of potato, air pressure and humidity. When measuring the volumes of water and Hydrogen Peroxide, the measurement should be taken from a 90-degree angle to avoid parallax error. Predictions I predict that as the substrate concentration increases, the rate of reaction will go up at a directionally proportional rate until the solution becomes saturated with the substrate Hydrogen Peroxide. ...read more.

Conclusion

For example: 1. There is a slight delay between pouring the thistle funnel with the potato discs into the beaker of Hydrogen Peroxide. This will slightly affect all the results for each individual experiment but as I carried out all the steps in the same way, it should not make any negotiable difference to the overall result. 2. It is also impossible to precisely measure out the amounts of Hydrogen Peroxide and Distilled Water each time. As the scale on the measuring cylinder shows the measurement to the nearest 1mm3, the solutions that I used should be correct to the nearest mm3. Ways to Improve Experiment. As using catalase founded in potatoes, the desired amount was hard to measure, however, measuring the amount of potato wasn�t difficult although that piece could contain different amounts of catalase compared to another piece. The results that would show from both pieces would be negotiable. Maybe having a source of catalase from yeast would have been much more easier to use and handle. Specific amounts could be measured much more precisely. ...read more.

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