• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Examine how the concentration of the substrate Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase.

Extracts from this document...


Aims This is an experiment to examine how the concentration of the substrate Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase. Background Information Enzymes such as Catalase are protein molecules, which are found in living cells. They are used to speed up specific reaction within the cell. They are all very specific as each enzyme just performs one particular reaction. Catalase is an enzyme found in food such as potato and liver. It is used for removing Hydrogen Peroxide from cells. Hydrogen Peroxide is the poisonous by-product of metabolism. Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen as shown in the equations below. Apparatus Need For The Experiment. 250 cm3 Glass Beakers, Glass Thistle Funnel, Graduated Measuring Cylinder, .Cork Borer,250 cm3 Glass Cylinders , Digital Stop clock, Scalpel, Tap , Distilled Water, Plastic rule, Safety Goggles. Method To test out how the concentration of Hydrogen Peroxide affects the rate of reaction first set up the apparatus and prepare the different concentrations of Hydrogen peroxide as below: Concentration of Hydrogen Peroxide Volume of Hydrogen Peroxide (cm3) ...read more.


This gives the rate in cm3 of oxygen produced in the time of 5 minutes, this is because I am timing how much oxygen gas is given off in the space of 5 minutes for the various concentrations of Hydrogen Peroxide. From these results, a graph can be plotted with concentration on the x-axis and the volume of gas released on the y-axis. To ensure this experiment is completed as fairly as possible, all the variables except for the concentration of Hydrogen Peroxide must be kept the same for all experiments. Variables that must not be altered include: Temperature, Catalase Concentration, dimensions of potato, air pressure and humidity. When measuring the volumes of water and Hydrogen Peroxide, the measurement should be taken from a 90-degree angle to avoid parallax error. Predictions I predict that as the substrate concentration increases, the rate of reaction will go up at a directionally proportional rate until the solution becomes saturated with the substrate Hydrogen Peroxide. ...read more.


For example: 1. There is a slight delay between pouring the thistle funnel with the potato discs into the beaker of Hydrogen Peroxide. This will slightly affect all the results for each individual experiment but as I carried out all the steps in the same way, it should not make any negotiable difference to the overall result. 2. It is also impossible to precisely measure out the amounts of Hydrogen Peroxide and Distilled Water each time. As the scale on the measuring cylinder shows the measurement to the nearest 1mm3, the solutions that I used should be correct to the nearest mm3. Ways to Improve Experiment. As using catalase founded in potatoes, the desired amount was hard to measure, however, measuring the amount of potato wasn�t difficult although that piece could contain different amounts of catalase compared to another piece. The results that would show from both pieces would be negotiable. Maybe having a source of catalase from yeast would have been much more easier to use and handle. Specific amounts could be measured much more precisely. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. An experiment to investigate how a substrate affects the rate of reaction of the ...

    * Concentration of the substrate: The concentration of the substrate that is used in the reaction will affect the rate at which the two reactants react. This is because the more concentrated the substrate is the more particles there are, this increases the frequency at which they collide the opposite is also true.

  2. An Investigation into the effect of substrate concentration (H2O2) on the rate of breakdown ...

    * Apparatus- The apparatus will not be changed at all, apart from the use of different syringes for the Hydrogen Peroxide and Yeast respectively. I will be taking three readings at each concentration, in order to obtain more accurate results.

  1. The aim of my experiment is to investigate how the mass of potato (concentration ...

    It also helped me on deciding suitable apparatus to use for my real experiment. A few potatoes were minced using a mincing machine. 5g of minced potato were then obtained using a balance. I transferred this into a conical flask but with real difficulty.

  2. To examine the effect of Substrate Concentration (Hydrogen Peroxide) on the rate of an ...

    Place the potato inside the conical flask and place the conical flask itself inside the water bath Pour 30cm3 of 10 Vol. Hydrogen Peroxide into a 100cm3 beaker and also place this inside the water bath. The hydrogen peroxide is kept separately at this point to prevent the enzyme and substrate reacting before the desired time.

  1. To determine the relationship between surface area and rate of oxygen production when a ...

    The skin of the potato will be removed. The experiments be carried out at room temperature and will be monitored with a thermometer. I will throughout all my experiments keep consistence and the same order of perpetration. If these variables were not controlled I would not get accurate results because there would be no consistence and results may differ due to change in variables.

  2. Investigation on the effect of the concentration of hydrogen peroxide on the rate of ...

    The enzymes can't work faster if more substrates are added but they have to 'queue up' for an active site to be free and the enzymes are working at their maximum rate (Vmax). * This graph taken from the OCR Biology 1 shows the curves of oxygen released against time.

  1. An Investigation into how the varying concentration of a substrate affects the rate of ...

    The graph would look like this: Concentration of enzyme If you increase the concentration of the enzyme, the rate of reaction will be quicker. This is because there is more enzyme and so more molecules per cm�, which means there's a higher chance of he molecules colliding with the substrate molecules.

  2. Biology-2 QWC Practice Questions & Answers

    Explain the effect of ph and temperature on enzyme activity All enzymes work at an optimum ph and temperature. The optimum ph varies depending on the particular enzyme and where it works in the body, for example, enzymes in the stomach will want to work in acidic conditions so

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work