• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Experiment to investigate the activity of enzyme catalase at different pHs.

Extracts from this document...

Introduction

Experiment to investigate the activity of enzyme catalase at different pHs Prediction I predict that a pH of 7 will be the optimum Ph for the reaction and will give off the most bubbles. This is because enzymes have actives sites, which are affected by Ph. The active site fits the substrate allowing the reaction to work at it's best. When there is strong acid or alkali in the solution the active site is denatured changing the shape of the active site so that it is harder for the substrate to fit it, this slows or even stops the reaction. Hydrogen Peroxide Water + Oxygen This is the reaction I am going to investigate. Using different Buffer Solutions- solutions of differing pH values will alter the pH of the reaction. The optimum pH for the reaction will be the one, which produces the most bubbles. The more bubble given off the quicker the rate of reaction. Variables The variables I am going to change will be the pH buffer testing each pH 3 times. ...read more.

Middle

* Placed the bung on top of the measuring cylinder with the hydrogen peroxide, mushroom and pH buffer in. * Started the stopwatch * Started counting the bubbles * Stopped counting after 2 minutes * Recorded the results Trial Run Results These are the results I found using two different concentrations: Concentration Bubbles 10% 68 20% 97 As you can see the rate of reaction was much greater using 20% concentration, so this will be the one we will use for our experiments. Results Try 1 Try 2 Try 3 Average PH 2 8 9 7 8 PH 4 27 43 31 34 PH 7 91 101 83 82 PH 9 82 107 92 94 PH 12 1 0 3 1 These are the results of the enzyme catalase reaction with 1g of mushroom. We used hydrogen peroxide with a 20% concentration for the reactions. We put 15 cm of hydrogen peroxide in a test tube with 1g of mushroom and 15cm of ph buffer. ...read more.

Conclusion

Namely the fact different types of mushrooms were used. This may have had major affects on the accuracy of our pH averages. I did not find any anomalous results in my repeats for each pH as the results were consistent with each other so I am inclined to believe the change in mushroom type had not adverse affects on the overall reliability of my experiment. In some places my experimental process was weak. Instead of counting the bubbles given off, to make the experiment more reliable I could have measured the exact volume of the gas given off using a gas syringe. As I have stated my repeats for each pH were close in value. Though they do not support my prediction as pH 9 gave off the most bubbles. My results show a clear trend in enzyme activity and show that pH 9 gave off only marginally more bubbles. Testing all the pH values from 1-12 could extend the experiment. This would give a wider band of results making the experiment more accurate again. This would also confirm the theory further. I could also test different sources of the enzyme catalase e.g. (liver) and see if the pattern was similar. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. The effect of aspirin on the action of bovine liver catalase

    Limitations of the technique The main problem experienced with this technique was that the movement of the manometer bead was never steady. The bead would stay still for a while and then jump to the next point, this is probably the main reason for variability within the results.

  2. To investigate the effect of ph on the activity of trypsin.

    Also as a bunsen burner is to be used in the experiment I must be careful when it is alight. Fair Test: The experiment must be a fair test or I will be getting a lot of anomalous results and there may not be a clear pattern in the results.

  1. Influence of pH on the Activity of Potato Catalase.

    complex with the substrate and producing the by-products H�O and O�, at a faster rate. The least effective pH buffer to use in a Catalase reaction would be a pH of 2.8, although there may be more inhibiting pHs outside the range used in the experiment.

  2. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    Initial Reading (cm3): Volume of Oxygen: AVERAGE Initial Reading: AVERAGE Volume of Oxygen: 0 46.1 0 47.9 0 47 0 2 39.4 6.7 37.9 10 38.7 8.4 4 35.5 10.6 35.9 12 35.7 11.3 6 32.7 13.4 33.1 14.8 32.9 14.1 8 30.4 15.7 30 17.9 30.2 16.8 10 28.9

  1. Free essay

    Close Your Eyes

    I sat up and rubbed my eyes. My headache had died down slightly but I could still feel it there. I glanced at the table and saw a note. It was from Dougie. I read what it said. Louise, Hope you had a nice sleep.

  2. THE EFFECT OF BILE SALT ON THE ACTION OF THE ENZYME LIPASE

    pH for 4% Bile Trial 1 Trial 2 Trial 3 mean/average 0 10 10 10 10 30 10 9.9 9.8 9.9 60 10 9.9 9.5 9.8 90 9.1 8 8.4 8.5 120 8.8 7.7 8 8.1 150 8.7 7.6 7.7 8 180 8.6 7.5 7.5 7.8 210 8.5 7.4 7.4

  1. To investigate and compare the effect of temperature on the activity of catalase in ...

    The inhibitor molecule doesn't take part in any reaction, but its presence in the active site means the substrate cannot enter the active site. Non-completive irreversible inhibition is when chemical reagents combine permanently with the enzyme and this change the structure of the enzyme, causing it to lose its specificity.

  2. Free essay

    Aim: to investigate the effect of Ho concentration on the activity of the enzymes ...

    Therefore increase the rate of reaction. To do this, I will keep my sample in a water bath, so that the temperature will constant. Preliminary work Apparatus * G-clamp * Boiling tubes * Syringes * Measuring cylinder * Water * Clamp stand * Delivery tube * Stopwatch * Rubber bungs Method 1.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work