Experiment to investigate the activity of enzyme catalase at different pHs
Prediction
I predict that a pH of 7 will be the optimum Ph for the reaction and will give off the most bubbles. This is because enzymes have actives sites, which are affected by Ph. The active site fits the substrate allowing the reaction to work at it’s best. When there is strong acid or alkali in the solution the active site is denatured changing the shape of the active site so that it is harder for the substrate to fit it, this slows or even stops the reaction.
Hydrogen Peroxide Water + Oxygen
This is the reaction I am going to investigate. Using different Buffer Solutions- solutions of differing pH values will alter the pH of the reaction. The optimum pH for the reaction will be the one, which produces the most bubbles. The more bubble given off the quicker the rate of reaction.