• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Experiment to investigate the effect of Catalase on H2O2 at different temperatures.

Extracts from this document...

Introduction

Experiment to investigate the effect of Catalase on H2O2 at different temperatures Aim The aim of this experiment is to find out how temperature effects the rate of a reaction between Hydrogen Peroxide and yeast. Prediction I predict that as temperature increases so will the rate of the reaction (up to a certain temperature, at which it will no longer 'function'). Once the temperature gets to 50�C, the enzyme breaks down and stops working. However, rises in temperature of 10�C should be expected to double the rate of reaction. Background Information Yeast is an enzyme, an enzyme is a biological Catalyst made up of protein which speed up different chemical reactions without being used up themselves. Enzymes have a very unique shape. This unique shape only reacts with one or two substances. When it does react with a substance molecules of that substance get trapped on the Active Site and either creates two molecules (Anabolism) or splits up a molecule into two (Catabolism). When the molecules are trapped they, therefore, collide more frequently and therefore increase the rate of reaction. Basically, enzymes speed up the rate of a reaction by lowering the activity energy. Below is a diagram of an enzyme, notice the unique shape which only certain molecules will react with. The enzymes work at their best at 37�C (average human body temperature). ...read more.

Middle

* Simultaneously the Hydrogen peroxide will be added, the stopper placed on the boiling tube and then stop watch started. * We will then measure the amount of gas produced every 10 seconds, recording our results up to and including 200 seconds. * We will then change the temperature of the water bath to varying temperatures listed in the results table and repeat the experiment. Results Time (seconds) Experiment 1 - 17�C Experiment 2 - 38�C Experiment 3 - 13�C Experiment 4 - 60�C Experiment 5 - 48�C 1�� 2 0 0 0 0 0 0 0 10 0 0 0 0 0 1 20 8 5 7 4.5 16 9 30 16 11 16 10 25 16 40 22 15 21 14.5 29 22 50 26 19 25 18 32 25.5 60 29 22 28 21.5 34.5 28.5 70 31 24 30 22.5 36 30.5 80 33 26 32 25 37 32.5 90 34 27 33 27 37.5 33 100 35 28 33 28 38 34 110 35.5 28.5 33 28.5 38.5 34.5 120 36 29 34 29 39 35.5 130 36.5 29.5 35 30 39.5 36 140 36 30 35 30.5 40 36 150 36 30.5 35 31 40 36.5 160 36 31 35 31.5 40 37 170 36 31 35 32 40.5 37 180 36 31 35 32.5 40.5 37.5 190 36 31 35 33 41 37.5 200 36 31 35 33 41 38 Analysis of Graph The graph is made up of several curves, which start at the origin. ...read more.

Conclusion

However, they are not off by much at all. To test my results for lack of accuracy I did the first experiment twice and compared those results. From this I found that the end amount of gas produced was 5cm� more than it was the second time I did the experiment. Errors in the experiment can be due to several factors. These factors are the 'key' factors mentioned above. These key factors are factors that could change the results by varying amounts if they are not constant. These factors are basically: * Time * Temperature * Amount of Hydrogen Peroxide used * pH of the solution * Shaking of the test tube We tried to keep these values at a constant and tried to repeat each experiment exactly the same as the last one. However, in experiments where readings are required to be read off a scale there will be a lot of human error. Improving the Experiment There are only a few ways in which we could improve the experiment, and it is likely the highest sources of error came from human error. However, we could always increase the accuracy of the results by using more accurate equipment, such as an Electro thermostatically controlled water baths which are precise to 0.05�C. Gas syringes would also be a possibility to us. Whether gas syringes would be more accurate or not is not sure, but the scales might be easier to read and therefore reduce human error. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. The Decomposition of H2O2 using Catalase, in yeast as a catalyst.

    After this temperature is reached, the rate will decrease until it stops altogether due to the Catalase being denatured. Pre-tests In order to find the most effective method I decided to carry out some pre-tests. These helped me to discover the most accurate and safest way to carry out this experiment.

  2. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    Insert a delivery tube up the burette; this must be connected to a stopper. 8. Using a pipette, extract the liquid from the bottom of the tube and place into a measuring cylinder to determine a percentage of the catalase that you want to test (measurements must be up to 10cm3).

  1. THE EFFECT OF BILE SALT ON THE ACTION OF THE ENZYME LIPASE

    This can simply be done by shaking the beakers or flasks gently which contains the solutions ensuring nothing spills. 5. Once the above has been carried out, ensure the probe is placed in distilled water, and then set the pH on the data logger ensuring it reads 7.0 pH as this is the pH of distilled water.

  2. To investigate the effect of changing concentration of Hydrogen Peroxide (H2O2) on the enzyme ...

    Plan The aim of the investigation is to find out if changing the concentration of the substrate will change the rate of reaction. The variable I am going to change is the concentration of hydrogen peroxide. Variable to be kept the same Reason Volume To keep the rate the same (different variable).

  1. To investigate and compare the effect of temperature on the activity of catalase in ...

    The theoretically possible maximum rate (Vmax) is never achieved, but the reaction eventually reaches a point when increasing substrate concentration only gives negligible change in rate. The explanation for this is that almost all the active sites in the enzyme molecules are filled because of the high concentration of substrate.

  2. Free essay

    Aim: to investigate the effect of Ho concentration on the activity of the enzymes ...

    48 240 37 53 In this experiment, I measured the volume of oxygen produced by counting the number of bubbles produced. The higher the number of bubbles produced in 30 seconds meant a faster reaction rate. Improvement Through this experiment, I found out that the method I used is not

  1. To determine the rate law for a chemical reaction among hydrogen peroxide, iodide and ...

    This prevented any readily apparent reaction of equation (III). However, after all the thiosulfate ions had been consumed by the reaction of equation (II), triiodide ions reacted with starch to form the blue starch-pentaiodide complex. By varying the concentration of each of the three reactants (H2O2, I- and H+), we will be able to determine the order of the

  2. Factors Affecting Enzyme Activity

    I think there will be a relationship of proportionality between the concentration of the suspension and the rate of reaction. Below is a predicted graph of results (all measurements are in arbitrary units). I have plotted all five graphs on one set of axes as this will make my results easier to analyse once I have collected them.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work