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Experiment to investigate the effect of Catalase on H2O2 at different temperatures.

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Introduction

Experiment to investigate the effect of Catalase on H2O2 at different temperatures Aim The aim of this experiment is to find out how temperature effects the rate of a reaction between Hydrogen Peroxide and yeast. Prediction I predict that as temperature increases so will the rate of the reaction (up to a certain temperature, at which it will no longer 'function'). Once the temperature gets to 50�C, the enzyme breaks down and stops working. However, rises in temperature of 10�C should be expected to double the rate of reaction. Background Information Yeast is an enzyme, an enzyme is a biological Catalyst made up of protein which speed up different chemical reactions without being used up themselves. Enzymes have a very unique shape. This unique shape only reacts with one or two substances. When it does react with a substance molecules of that substance get trapped on the Active Site and either creates two molecules (Anabolism) or splits up a molecule into two (Catabolism). When the molecules are trapped they, therefore, collide more frequently and therefore increase the rate of reaction. Basically, enzymes speed up the rate of a reaction by lowering the activity energy. Below is a diagram of an enzyme, notice the unique shape which only certain molecules will react with. The enzymes work at their best at 37�C (average human body temperature). ...read more.

Middle

* Simultaneously the Hydrogen peroxide will be added, the stopper placed on the boiling tube and then stop watch started. * We will then measure the amount of gas produced every 10 seconds, recording our results up to and including 200 seconds. * We will then change the temperature of the water bath to varying temperatures listed in the results table and repeat the experiment. Results Time (seconds) Experiment 1 - 17�C Experiment 2 - 38�C Experiment 3 - 13�C Experiment 4 - 60�C Experiment 5 - 48�C 1�� 2 0 0 0 0 0 0 0 10 0 0 0 0 0 1 20 8 5 7 4.5 16 9 30 16 11 16 10 25 16 40 22 15 21 14.5 29 22 50 26 19 25 18 32 25.5 60 29 22 28 21.5 34.5 28.5 70 31 24 30 22.5 36 30.5 80 33 26 32 25 37 32.5 90 34 27 33 27 37.5 33 100 35 28 33 28 38 34 110 35.5 28.5 33 28.5 38.5 34.5 120 36 29 34 29 39 35.5 130 36.5 29.5 35 30 39.5 36 140 36 30 35 30.5 40 36 150 36 30.5 35 31 40 36.5 160 36 31 35 31.5 40 37 170 36 31 35 32 40.5 37 180 36 31 35 32.5 40.5 37.5 190 36 31 35 33 41 37.5 200 36 31 35 33 41 38 Analysis of Graph The graph is made up of several curves, which start at the origin. ...read more.

Conclusion

However, they are not off by much at all. To test my results for lack of accuracy I did the first experiment twice and compared those results. From this I found that the end amount of gas produced was 5cm� more than it was the second time I did the experiment. Errors in the experiment can be due to several factors. These factors are the 'key' factors mentioned above. These key factors are factors that could change the results by varying amounts if they are not constant. These factors are basically: * Time * Temperature * Amount of Hydrogen Peroxide used * pH of the solution * Shaking of the test tube We tried to keep these values at a constant and tried to repeat each experiment exactly the same as the last one. However, in experiments where readings are required to be read off a scale there will be a lot of human error. Improving the Experiment There are only a few ways in which we could improve the experiment, and it is likely the highest sources of error came from human error. However, we could always increase the accuracy of the results by using more accurate equipment, such as an Electro thermostatically controlled water baths which are precise to 0.05�C. Gas syringes would also be a possibility to us. Whether gas syringes would be more accurate or not is not sure, but the scales might be easier to read and therefore reduce human error. ...read more.

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