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How do we prove bacteria are ubiquitous?

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Introduction

Problem: How do we prove bacteria are ubiquitous? Introduction: 1) How is a bacteria colony formed? A bacteria colony is formed by a bacterium dividing exponentially. 2) What is meant by the term "ubiquitous"? The term ubiquitous is the state being or seeming to be everywhere at the same time. Relating this term to microbiology, bacteria are so diverse and ubiquitous; they can be isolated and grown from any environment. 3) What practical steps can be taken in the lab to prevent contamination? A sterile environment is the best step that should be taken to prevent contamination. One should also wear gloves, a lab coat, goggles, and a hair net to prevent hair from falling into experiments. One should also bleach the surface area and wash equipment before and after experiments. ...read more.

Middle

* Masking tape * Worksheet of colony descriptions Procedure: * Donne gloves, lab coat, goggles. * Bleach table top. * Set up hot water bath (ring stand, wire gauze, beaker 3/4 full of H2O. * When beaker starts to boil add agar test tube butt (be sure to remove cap first). * Wait until agar melts (it will become a golden yellow liquid). * Obtain sterile Petri dish. * Using a wax pencil, label all four quadrants of Petri dish. * Using your best sterile technique, pour the agar into the Petri dish, partially replace the cover. Wait until it gels (do not touch inside of the dish cover or bottom). * After the gel cools/ hardens, obtain a sterile cotton swab * Each student at the table will inoculate on of ...read more.

Conclusion

* Inoculate the inverted Petri dish for 48 hours at 34 degrees Celsius. * Do not open the Petri dish. * After 48 hours observe Petri dish with magnifying lens. * Draw observations. Conclusion: 1) What is a bacterial colony? A bacterial colony is when there is a visible growth of microorganisms. Bacterial colonies grow in many forms. 2) Why is the Petri dish inverted in the incubator? To prevent condensation from forming, the dishes must be placed inversely in the incubator. 3) List terms and define the term used to describe colonial morphology? Colonial morphology refers to the form and structure of organisms. Such terms include the colony's surface which can be smooth, rough, and wrinkled. Optical characteristics include translucent, dull, glossy, multi-colored, and hazy looking colonies. Most importantly the forms of bacteria, which include, circular, irregular, spindle, filamentous, and rhizoid shapes. ...read more.

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