• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  4. 4
    4
  5. 5
    5
  6. 6
    6
  7. 7
    7
  8. 8
    8
  9. 9
    9
  10. 10
    10
  11. 11
    11
  12. 12
    12
  13. 13
    13
  14. 14
    14
  15. 15
    15
  16. 16
    16

HOW DOES TEMPERATURE AFFECT CATALASE ENZYMES?

Extracts from this document...

Introduction

HOW DOES TEMPERATURE AFFECT CATALASE ENZYMES? Aim. To investigate the affect of temperature on catalase enzyme activity, using a potato as a catalyst. The source of catalase is in the potato cells. Theory. I have done some research to find out about enzymes in general and about catalase. Enzyme is a protein molecule that speeds up chemical reactions in all living things. Without enzymes, these reactions would occur too slowly or not at all, and no life would be possible. Many enzymes break down complex substances into simpler ones. Others build complex compounds from simple ones. Most enzymes remain in the cells where they were formed, but some enzymes work elsewhere. For example, the pancreas secretes the enzyme lipase, which travels to the small intestine, where it breaks down fats. An enzyme's structure can easily be destroyed by heat, or PH. For example, scientists believe that a high body temperature, such as 42 �C, may cause death because the heat makes vital enzymes inactive. Enzymes have many uses in addition to their natural functions in the body. Manufacturers use enzymes in making a wide variety of products. For example, some detergents contain enzymes that break down proteins or fats that cause stains. Enzymes are also used in the manufacture of antibiotics, beer, bread, cheese, coffee, sugars, vinegar, vitamins, and many other products. Doctors use medicines containing enzymes to help clean wounds, dissolve blood clots, relieve certain forms of leukaemia, and check allergic reactions to penicillin. Doctors also diagnose some diseases by measuring the amount of various enzymes in blood and other body fluids. Such diseases include anaemia, cancer, leukaemia, and heart and liver ailments. All living cells make enzymes, but enzymes are not alive. Enzyme molecules function by altering other molecules. Enzymes combine with the altered molecules to form a complex molecular structure in which chemical reactions take place. Enzymes do not undergo any permanent chemical change of their own. The enzyme, which remains unchanged, then separates from the product of the reaction. ...read more.

Middle

IMPORTANT NOTE: check that the bottle top of gas syringe fits selected conical flask. 7. Grate a potato using a grater on a tile. The potato should be grated on the medium sized grater. The potato should not be in contact with the table in case it is mixed with any other substances already on the table. 8. Leave this grated potato in a large beaker. 9. Measure out 5g of grated potato and put it in the conical flask using a spatula. 10. Using the measuring cylinder, measure 30ml of hydrogen peroxide. 11. When pouring hydrogen peroxide into the conical flask, make sure you don't spill any of it on your hand. If you happen to accidentally spill some, wash it off quickly under cold, running water. 12. Get the stop watch ready. 13. Reset the timer. 14. Pour in the 20ml of hydrogen peroxide into the conical flask. 15. Push the gas syringe into the top of the conical flask instantly and start the stopwatch immediately. 16. Repeat this with six other temperatures for the hydrogen peroxide to get more accurate results and also to make it a fair test. The different temperatures should be 0�C, 10�C, 20�C, 30�C, 40�C and 50�C. So we can see which temperature the enzymes work best at. 17. For each experiment, take down the results after every 30 seconds. Below is a diagram show how the experiment should be presented. Safety It is vital that while I am doing this experiment I am safe, the environment around is safe as well as people around me so I have made a list of safety precautions that I will take. 1. Wear safety goggles at all times to protect my eyes. 2. Keep my fingers away when grating the potato to avoid harming my fingers. 3. Not run with equipment especially equipments made of glass e.g. beakers. 4. ...read more.

Conclusion

I also think it would have been better if I had used the same potato from the whole experiment but was unable to due to the time restrictions. I had to conduct the experiment over a number of days and could not therefore use the same potato. This is a source of error because the concentration of catalase in the potatoes may have been different which may have produced an inconsistent rate of reaction. This might be one of the reasons in which my results were inaccurate. To remove this problem, I could repeat the experiment not only with three readings at each temperature, but also with three different potatoes, which would provide an even more accurate reading, as I could calculate an average. I would also carry out more experiments with a range of 5�C. I would do 0�C-70�C. By 70�C the enzyme would have denature. I would have carried out more experiments because this would give me more results and allow me to plot a more accurate graph. Also, it would be easier to tell when the enzyme got to the optimum temperature and denaturing point. So next time I would do all the things below to ensure even more accurate results; * Tighten the bung on the flask. * Use a good gas syringe (not too tight or loose) * Use the same equipments apart from the flask. * Do all the experiments on the same day using the same potato. * Use three different potatoes for each of the experiments so; I could calculate an even better average. * Make sure the water bath is working. * Carry out more experiment, with a temperature range of 5�C. Conclusion To summarize I found out that enzymes work fastest at 10�C even though according to my theory it should be 40�C. I also found out that it is correct about how the enzyme denatures after 40�C. So enzyme activity is affected by temperature. The optimum temperature for enzymes should be 40�C, and after this they should not work well as they are denatured. Aysha Sattar, 10b, 10/12/03 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Thyroid Cancer Research Project

    3 star(s)

    2.3)Alternative solutions: Chemotherapy has a limited role in the treatment in the treatment of advanced radio-iodine resistant cancers,such as PDTC,MTC,and ATC.Patients with advanced or metastatic thyroid cancer should be considered for clinical trials of new agents such as tyrosine kinase inhibitors,antiangiogenesis agents and growth modulators.(16)

  2. Peer reviewed

    The Journey of a cheese sandwich

    4 star(s)

    The food empties from the stomach into the duodenum where it is mixed with enzymes and salts produced by the liver and enzymes made in the pancreas. The pancreas secretes three main enzyme types: protease enzymes, carbohydrase enzymes and also lipase enzymes run into the duodenum at the top the small intestine, where it continues its journey.

  1. The aim of this experiment is to demonstrate that the substrate Hydrogen Peroxide will ...

    Therefore, I compared the surface area of the potato (as identified in Table 1) with the timings and production of Oxygen. With this information, I was then able to calculate the time it takes for the reaction and link this to the surface area of the potato.

  2. The effect of temperature upon the enzyme catalase

    20oC however the temperature in the room at the time was 23oC. The final temperature, 74oC, was supposed to be 80oC however the water tank we used was only able to sustain 74oC. To fix that problem we should have used a different water tank which was capable of sustaining a higher temperature.

  1. Experiment to investigate the effect of Temperature on the enzyme activity of Pectinase

    The logical explanation of why the enzyme denatures would be that if the pH is decreased too much, there would be too many H+ ions around the protein and thus the H+ ions would be attracted to the places in the enzyme which were more negative than the enzyme, thus forming a hydrogen bond there.

  2. What influence does pH have on the enzyme Catalase?

    Therefore, the reaction the enzyme catalyses will not take place. This change of the active site shape is called denaturing of the enzyme. When we alter the pH we are changing the concentration of the hydrogen ions in a solution.

  1. Investagating the Action of the Enzyme Catalase On the Surface Area of a Potato.

    The molecule of the substrate hydrogen peroxide has to fit in to the contours of a region in the catalase enzyme molecule called the active site. The shape of the catalase enzyme and the active site of the enzyme catalase let the molecule of the substrate fit in and lock as a key.

  2. Research Project - Comparing Antiseptics, Antibiotics and Disinfectants

    The antiseptics that kill bacteria outright are known as bacterial, the others, described as bacteriostatic, stop bacteria from growing, but do not kill them. http://www.faqs.org/health/topics/76/Antiseptics.html Comparing Antiseptics, Antibiotics and Disinfectants The main differences between antiseptics and disinfectants are that disinfectants are used mainly to sanitize objects, and antiseptics are used on human skin.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work