• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  4. 4
    4
  5. 5
    5
  6. 6
    6
  7. 7
    7
  8. 8
    8
  9. 9
    9
  10. 10
    10
  11. 11
    11
  12. 12
    12
  13. 13
    13
  14. 14
    14
  15. 15
    15
  16. 16
    16

HOW DOES TEMPERATURE AFFECT CATALASE ENZYMES?

Extracts from this document...

Introduction

HOW DOES TEMPERATURE AFFECT CATALASE ENZYMES? Aim. To investigate the affect of temperature on catalase enzyme activity, using a potato as a catalyst. The source of catalase is in the potato cells. Theory. I have done some research to find out about enzymes in general and about catalase. Enzyme is a protein molecule that speeds up chemical reactions in all living things. Without enzymes, these reactions would occur too slowly or not at all, and no life would be possible. Many enzymes break down complex substances into simpler ones. Others build complex compounds from simple ones. Most enzymes remain in the cells where they were formed, but some enzymes work elsewhere. For example, the pancreas secretes the enzyme lipase, which travels to the small intestine, where it breaks down fats. An enzyme's structure can easily be destroyed by heat, or PH. For example, scientists believe that a high body temperature, such as 42 �C, may cause death because the heat makes vital enzymes inactive. Enzymes have many uses in addition to their natural functions in the body. Manufacturers use enzymes in making a wide variety of products. For example, some detergents contain enzymes that break down proteins or fats that cause stains. Enzymes are also used in the manufacture of antibiotics, beer, bread, cheese, coffee, sugars, vinegar, vitamins, and many other products. Doctors use medicines containing enzymes to help clean wounds, dissolve blood clots, relieve certain forms of leukaemia, and check allergic reactions to penicillin. Doctors also diagnose some diseases by measuring the amount of various enzymes in blood and other body fluids. Such diseases include anaemia, cancer, leukaemia, and heart and liver ailments. All living cells make enzymes, but enzymes are not alive. Enzyme molecules function by altering other molecules. Enzymes combine with the altered molecules to form a complex molecular structure in which chemical reactions take place. Enzymes do not undergo any permanent chemical change of their own. The enzyme, which remains unchanged, then separates from the product of the reaction. ...read more.

Middle

IMPORTANT NOTE: check that the bottle top of gas syringe fits selected conical flask. 7. Grate a potato using a grater on a tile. The potato should be grated on the medium sized grater. The potato should not be in contact with the table in case it is mixed with any other substances already on the table. 8. Leave this grated potato in a large beaker. 9. Measure out 5g of grated potato and put it in the conical flask using a spatula. 10. Using the measuring cylinder, measure 30ml of hydrogen peroxide. 11. When pouring hydrogen peroxide into the conical flask, make sure you don't spill any of it on your hand. If you happen to accidentally spill some, wash it off quickly under cold, running water. 12. Get the stop watch ready. 13. Reset the timer. 14. Pour in the 20ml of hydrogen peroxide into the conical flask. 15. Push the gas syringe into the top of the conical flask instantly and start the stopwatch immediately. 16. Repeat this with six other temperatures for the hydrogen peroxide to get more accurate results and also to make it a fair test. The different temperatures should be 0�C, 10�C, 20�C, 30�C, 40�C and 50�C. So we can see which temperature the enzymes work best at. 17. For each experiment, take down the results after every 30 seconds. Below is a diagram show how the experiment should be presented. Safety It is vital that while I am doing this experiment I am safe, the environment around is safe as well as people around me so I have made a list of safety precautions that I will take. 1. Wear safety goggles at all times to protect my eyes. 2. Keep my fingers away when grating the potato to avoid harming my fingers. 3. Not run with equipment especially equipments made of glass e.g. beakers. 4. ...read more.

Conclusion

I also think it would have been better if I had used the same potato from the whole experiment but was unable to due to the time restrictions. I had to conduct the experiment over a number of days and could not therefore use the same potato. This is a source of error because the concentration of catalase in the potatoes may have been different which may have produced an inconsistent rate of reaction. This might be one of the reasons in which my results were inaccurate. To remove this problem, I could repeat the experiment not only with three readings at each temperature, but also with three different potatoes, which would provide an even more accurate reading, as I could calculate an average. I would also carry out more experiments with a range of 5�C. I would do 0�C-70�C. By 70�C the enzyme would have denature. I would have carried out more experiments because this would give me more results and allow me to plot a more accurate graph. Also, it would be easier to tell when the enzyme got to the optimum temperature and denaturing point. So next time I would do all the things below to ensure even more accurate results; * Tighten the bung on the flask. * Use a good gas syringe (not too tight or loose) * Use the same equipments apart from the flask. * Do all the experiments on the same day using the same potato. * Use three different potatoes for each of the experiments so; I could calculate an even better average. * Make sure the water bath is working. * Carry out more experiment, with a temperature range of 5�C. Conclusion To summarize I found out that enzymes work fastest at 10�C even though according to my theory it should be 40�C. I also found out that it is correct about how the enzyme denatures after 40�C. So enzyme activity is affected by temperature. The optimum temperature for enzymes should be 40�C, and after this they should not work well as they are denatured. Aysha Sattar, 10b, 10/12/03 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Investigating Enzymes and the effects of different variables such as temperature on how they ...

    4 star(s)

    However if it is too hot, the enzyme will denature, or lose its shape. If this happens then the reaction will not take place as the substrate must fit perfectly into the active site. The following diagram illustrates what happened in our reaction: Evaluation My results are mostly variable because

  2. Marked by a teacher

    Investigating the breakdown of hydrogen peroxide by the enzyme catalyse in potatoes.

    4 star(s)

    for more repeats of the experiment to acquire even more reliable results. To make our experiment more accurate, better and more advance methods could have taken place to stop human error in affecting the quality of data. Evaluation of Data It can be seen that the results I have obtained seem fairly reliable.

  1. Marked by a teacher

    Thyroid Cancer Research Project

    3 star(s)

    Chemotherapy can help doctors partially shrink cancerous growths, easing the pressure on surrounding tissues and improving organ function -- essentially increasing patient quality of life. Chemotherapy for pain relief, called palliative chemotherapy, is often given to patients with incurable cancer, according to the Colon Cancer Resource.(6)

  2. Peer reviewed

    The Journey of a cheese sandwich

    4 star(s)

    In the small intestine there are millions of villi. These are small, but visible to the human eye, finger like structures which line the small intestine and are perfect for absorbing the food. The main reason why villi are so good at absorbing food is that they have a thin layer of outer cells, which act as a cell, called micro-villi.

  1. The aim of this experiment is to demonstrate that the substrate Hydrogen Peroxide will ...

    Therefore, I compared the surface area of the potato (as identified in Table 1) with the timings and production of Oxygen. With this information, I was then able to calculate the time it takes for the reaction and link this to the surface area of the potato.

  2. The effect of temperature upon the enzyme catalase

    This would mean that the results were inaccurate due to a lack of precision. One way this could be improved would be to keep the enzyme within the water tank for a longer period of time and test the temperature with a thermometer to make sure it has been properly equilibrated.

  1. An Investigation into the effect of surface area on the activity of catalase in ...

    display of the stopwatch * Test-tube/boiling-tube rack To hold boiling tube Holds the boiling tube in a steady upright position whilst I am recording my results, or preparing for next experiment * Thermostatic water bath with water set to 38?C or above To heat hydrogen peroxide in boiling tube Water

  2. What influence does pH have on the enzyme Catalase?

    The active site is made of chain amino acids with atoms in a particular order to match the substrate. The particular series of the atoms in the amino acid chain at the active site form temporary ionic bonds to the substrate to hold onto it before it is broken down.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work