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How does temperature affect the way in which an enzyme works in Hydrogen Peroxide?

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Introduction

How does temperature affect the way in which an enzyme works in Hydrogen Peroxide? Introduction. The aim of this investigation is to find out how temperature affects the speed at which the enzyme,Catalase,works best in Hydrogen Peroxide.I hope to achieve reliable results that will confirm my predictions. I predict that:- * By increasing the temperature of the Hydrogen Peroxide,the Catalase will react faster. This is because the particles have more energy so they will move and react at a faster rate. * At about 40c, the Catalase will start to deteriorate because the substrate no longer fits the enzyme making the enzyme useless. * I predict that the enzyme will work at its best at 37c because that is the human body temperature. * With temperatures lower than 40c,I will expect the rate of reaction to increase gradually as the temperature is increased because the particles will move faster because of the increase of their energy therefore they will react faster. * With temperatures higher than 40c,I expect to see the rate of reaction decrease rapidly and the enzyme become denatured because the enzyme and substrate no longer fit together meaning the enzyme is denatured. * I also predict that when my results are put on to a graph they will look something like this... (rough graph to show what line of best fit should and will eventually look ) ...read more.

Middle

45 c 0 (drew up water) 55 c 0 (drew up water) I have decided to keep these results and compare them with the results I get when I do the actual experiment. Method. Collect the following apparatus: * Bunsen burner. * A boiling tube. * A test tube. * A beaker. * Rubber tubing attached to a boiling tube stopper. * A measuring cylinder. * Safety goggles. * Hydrogen peroxide. * A piece of potato. * A test tube holder. * A stopwatch. * A tripod. * A wire gauze. Put on your safety goggles. Set up the apparatus as follows: (apparatus once fully set up) * Fill the beaker with about 200ml of water. * Use the measuring cylinder to measure out 15cm3 of hydrogen peroxide and pour it into the boiling tube. * Place thermometer into the boiling tube and place the boiling tube into the beaker and heat to the desired temperature. * Fill the test tube with water but not too much as the some of the water may be taken up the rubber tubing. * Cut the potato to 2cms long making sure all skin is removed. Once you have heated the hydrogen peroxide to the temperature you want, place the boiling tube in a test tube holder making sure the rubber tubing can be inserted into the test tube containing water. ...read more.

Conclusion

to the desired temperature and then carry on with the experiment which meant that the temperature of the hydrogen peroxide fluctuated during the experiment which more than likely affected the overall results. Also,the hydrogen peroxide was measured in a plastic measuring cylinder which meant that the amount of hydrogen peroxide might not have benn the exact amount.If an electronic liquid measurer had been used,the amount of hydrogen peroxide would have been more accurate and also the results may have been more accurate. The distance the rubber tubing went into the water in the test tube would be another issue regarding the accuracy of theexperiment.Each of the five times the expeiment was carried out,the rubber tubing was always a different distance in the test tube which means the further in the tubig was,the more water was taken up.This would affect the results because the more water taken up by the rubber tubing,the longer and the more oxygen it would take to push the water out.This means that if the experiment was done 3-5 times at the same temperature,the amount of bubbles would be likely to change. --------------------------------------------------------------------------------------------------------- Out of the two methods used,this was probably the most reliable because it was clear and you could tell that gas was actualy being produced because the bubbles were very easy to see. I think this experiment was a fair test because it was timed each time with an accurate stopwatch.I am very pleased with the results I obtained. ...read more.

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