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In this experiment our intention is to test the effects of concentration upon a reaction involving hydrogen peroxide and an enzyme known as catalase

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Introduction

PLAN Aim In this experiment our intention is to test the effects of concentration upon a reaction involving hydrogen peroxide and an enzyme known as catalase - in which hydrogen peroxide is broken down in to oxygen and water aided by the enzyme, which is found in potato. Below is the following reaction which occurs in our experiment: The arrow represents the process of Catalysis; the change in rate of a chemical reaction due to the involvement of the previously motioned enzyme, catalase. Background The rate of reaction relies upon a number of principles to work. One of these being, collision theory - this states that in order for something to react it must collide with enough energy with something else. Another being, activation energy - the energy needed to break the bonds of the reactants and so allows new bonds to form the products. This can be shown through the use of an energy level diagram (below): Using the enzyme catalase also increases the rate of reaction because of the lowered energy of activation. This means more particles have the required energy of activation. ...read more.

Middle

was full of gas which allowed us to make use of the full range of this piece of equipment. Equipment List Below you will see all the equipment used in the experiment along with a diagram and an explanation of why we will use them. Equipment Name. Diagram. Explanation of Use. Gas Burette This provides a precise measurement of the gas produced due to the small increments of 1cm3 when compared to the syringe which displayed increments of 5cm3 Syringe Bucket Spatula Bung Delivery Tube Digital Balance Digital Stop-Clock Thermometre Potatoe & Hydrogen Peroxide ANALYSIS Results Table Concentration of H2O2 (%) Volume of O2 Created in 20 seconds (cm3) Mean (cm3) Mean Rate of Reaction (cm3/min) Test 1 Test 2 Test 3 25 7.5 8.2 7.9 7.86 23.58 50 16.1 16.6 15.8 16.16 48.48 75 24.4 21.3 22.7 22.96 68.88 100 35.5 42 37.1 38.2 114.6 My results convey a positive correlation between the concentration of hydrogen peroxide and the rate of its decomposition into liquid water and oxygen. All the points on my graph follow a similar pattern: as the concentration of hydrogen peroxide increases, the mean volume of oxygen produced within one minute also increases. ...read more.

Conclusion

The above diagram shows how an enzyme such as catalase comes in to contact with a substrate and the chemical reaction occurs. Step 1: The enzyme and substrate are separate at this point. Step 2: Now, the enzyme, catalase interacts with the hydrogen peroxide (the substrate) in order to create an enzyme-substrate complex as they are both complimentary towards each other. Step 3: Once, both the enzyme and substrate are joined together to make an enzyme-substrate complex, the active sight (high-lighted grey) alters shape somewhat, and places stress upon the molecular bonds of the substrate in a process named induced fit, this is the process in which the hydrogen peroxide (the substrate) undergoes a chemical reaction and turns in to the products of water and oxygen. This stress reduces the activation energy needed. Step 4: Finally, the products of water and oxygen are made and the recently occupied enzyme is now ready to repeat these steps. Also, only one enzyme is able to undergo one reaction at a time. This effect is responsible for the demonstrated change in the dependent variable, the speed of the reaction. ?? ?? ?? ?? Hydrogen Peroxide Experiment Science, Mrs. Heslop Liam Owens 1 | Page ...read more.

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