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In this investigation I am going to examine if the concentration of hydrogen peroxide affects the rate at which it is broken down by enzymes in the liver known as catylase.

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Introduction

In this investigation I am going to examine if the concentration of hydrogen peroxide affects the rate at which it is broken down by enzymes in the liver known as catylase. I think that the more hydrogen peroxide used (the higher the concentration), the more oxygen will be produced. I think this because I have researched enzymes and catalysts and have found out the following information on chemical reactions, enzymes, catalysts and concentration of acid : A chemical reaction is the process in which chemical substances are converted into other substances. A reactant is a chemical that can be converted into another substance through a chemical reaction. A catalyst is a substance that speeds up the rate of a chemical reaction without being used up itself. Before a reactant can turn into a product it needs the energy to start off the reaction. The energy needed to start a reaction is called activation energy. A catalyst lowers the activation energy, making it easier for particles to react. Using a catalyst a lot more particles have enough energy to react. Enzymes are biological catalysts. They are large molecules, each with their own special shape. Reactants fit into the enzymes active sight, and then broken down in to new products. ...read more.

Middle

Record your results each time. Now repeat the entire experiment twice more and record your results. To keep this investigation fair the experiment will need to carried out more than once (in this case 3 times) to gain better average results. The liver will need to be kept at the same temperature, as this could affect the rate at which the catalysts inside it work. The amount of hydrogen peroxide and water will need to be measured very carefully. The stopwatch will need to be started when the water and hydrogen peroxide are poured over the liver and stopped after exactly a minute. To keep this investigation safe plastic gloves will need to be worn to prevent hygrogen peroxide making contact with the skin and to stop any germs on the liver spreading. Safety glasses will also need to be worn to prevent hydrogen peroxide splashing into the eyes. After the experiment your hands will need to be washed incase any hydrogen peroxide did make contact with your skin. Here are the tables of the results from my three experiments: % Amount of Amount Total Amount Volume of Solution Hydrogen of Water of Solution Oxygen Produce Peroxide (ml) (ml) (ml) in 1 Minute (ml) ...read more.

Conclusion

Increasing the amount of hydrogen peroxide meant there would be more hydrogen particles in the same volume of solution. This would increase the chances of the particles colliding with the liver and reacting with it. A 10% Solution Of A 20% Solution Of Hydrogen Peroxide Hydrogen Peroxide When the solution is doubled there is twice as much chance of the hydrogen peroxide particles colliding with the liver. I did have 2 anomolous results. These could be wrong for number of reasons : There could have been a change in room temperature, causing enzymes in the liver to work at a different rate. Too much or not enough hydrogen peroxide or water could have been added. The stop-watch could have been started to early or to late, or could have been faulty. I could make my investigation better by : Doing my experiment more times to obtain better average results. Keeping the liver at the same temperature throughout the experiment. To investigate further I could : Investigate how temperature affects the way enzymes in the liver work. Investigate how much hydrogen peroxide would be needed to produce a certain amount of oxygen. Investigate how long it would take for hydrogen peroxide to produce a certain amount of oxygen. Investigate how the size or surface area of the liver would affect the amount of oxygen produced. ...read more.

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