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Investigate one of the fastest enzyme reactions in nature - This reaction involves the breakdown of hydrogen peroxide (a poison) by an enzyme (catalase) into non-poisonous water and oxygen.

Extracts from this document...

Introduction

Results to show 30mls of hydrogen peroxide to 0mls water Time 1st Result 2nd Result Average result 1 3 5 4 2 5 6 5.5 3 6 8 7 4 8 10 9 5 10 11 10.5 6 11 12 11.5 7 12 13 12.5 8 14 15 14.5 9 15 18 16.5 10 16 23 19.5 Results to show 25mls of hydrogen peroxide to 5mls of water Time 1st Result 2nd Result Average Result 1 2 6 4 2 3.5 8 5.75 3 4 10 7 4 5.5 12 8.75 5 6.5 15 10.75 6 7 16 11.5 7 8 17 12.5 8 9.5 18 13.75 9 10 18 14 10 11 20 15.5 Time 1st Result 2nd Result Average Result 1 2 5 3.5 2 3 7 5 3 5 10 7.5 4 8 14 11 5 8.5 16 12.25 6 9 18 13.5 7 9.5 18 13.75 8 10 18 14 9 10.5 18 14.25 10 11 18 14.5 Results to show 20mls of hydrogen peroxide to 10mls of water Results for 15mls hydrogen peroxide to 15mls of water Time 1st result 2nd Result Average Result 1 1.5 5 3.25 2 3 7 10 3 4 9 6.5 4 4.5 10 7.25 5 5 11 8 6 6 12 9 7 6.5 12 9.25 8 7.5 12 9.75 9 8 12 10 10 8 12 10 Results for 10mls of hydrogen peroxide to 20mls of water Time 1st Result 2nd Result Average Result 1 1 4 2.5 ...read more.

Middle

a mixture of solutions the reaction times would be slower as the liquids are moving at different speeds and the molecules collide in different ways, example glancing blows, which does little harm to the catalyst. Also at higher temperatures particles move faster so more collisions take place and they are more energetic so the reaction is faster. Chemical reactions also cause atoms to be rearranged by joining or separating to be able for atoms to join, a collision has to take place but this by itself wouldn't be enough. Bonds between atoms must be broken and new ones formed but this means that it takes a lot of energy for a successful collision to occur. This means there is a certain minimum energy needed before a reaction from a collision can occur this minimum energy is gained through higher concentration or a higher temperature. DETAILED PLAN FOR PRELIMINARY EXPERIMENT AND FINAL RESULTS We will use..... * Measuring cylinder * Delivery tube * Conical flask * Plastic container * Potato and Hydrogen peroxide This equipment will be set up as shown below We will use 4g of potato and add it to different amounts of concentration of hydrogen peroxide. * 30ml hydrogen peroxide 0ml water 25ml hydrogen peroxide 5ml water * 20ml hydrogen peroxide 10ml water 15ml hydrogen peroxide 15ml water * 10ml hydrogen peroxide 20ml water The ones marked with a bullet point are the amounts we used for preliminary testing. ...read more.

Conclusion

This shows that the two things are proportional to one another. EVALUATION What I predicted before the experiment was quite accurate. I predicted that as the concentration of hydrogen peroxide increased so would the rate of reaction. I feel the accuracy of my results were fairly good but for the 20mls of hydrogen peroxide to 10mls of water I think that between 3 minutes and 8 minutes the results don't fit the pattern. I can see this as the results show that more oxygen was produced while the expected results would have been less however towards the end the results level out and go against the expected pattern. To improve the experiment I would change a number of things. Although I felt at the time that it was accurate but I have since realized that these are things I could have improved. I would have made sure that I did the experiments more times because there would have been a better average. Also to get a more accurate set of results I would have made sure that no water had escaped from the measuring cylinder and made sure that I double checked everything making sure that only the one variable was certain to change. Final Time Average Rate of reaction 10 19.5 1.95 10 15.5 1.55 10 14.5 1.45 10 10 1 10 7 0.7 RATE OF REACTION = FINAL TIME AVERAGE OXYGEN PRODUCED ...read more.

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