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Investigate the effects of a concentrated sucrose solution on potato cells on the basis of the Osmosis theory.

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Introduction

GCSE biology investigation: Osmosis experiment Planning Aim: The main subject that I will be planning to investigate is the effects of a concentrated sucrose solution on potato cells on the basis of the Osmosis theory. Background knowledge: The plant cell and its structure To understand osmosis in detail I will need to explain the plant cell (which is the cell included in the osmosis experiment) and its cell membrane. Below I have a diagram of a plant cell: Osmosis is about the movement of particles from a higher concentrated solution to a lower concentrated solution to create an ethical balance via a partially or semi permeable cell membrane. Osmosis in simple terms is the exchange of particles between the cytoplasm inside the cell and the solution outside the cell. What makes this exhange is the partially permable cell membrane. This cell membrane plays an important part in Diffusion. Cell membrane and Diffusion Diffusion is the movement of the molecules of gas or liquids from a higher concentrated region to a lower concentration through the partially permeable cell membrane along a concentraion gradient. This explanation is in the diagram shown below: Turgor When a plant cell is placed in a dilute solution or a less concentrated solution then the water particles pass through the partially permeable membrane and fill the cell up with water. ...read more.

Middle

To ensure the success of the theory of the results need to be accurate, which leads us to fair testing. The first variable in the experiment that will need to be moderated is the room temperature. As with most scientific experiments temperature can affect the experiment, therefore the temperature in this particular experiment shall be kept at a room temperature, which is about 22-23C. Variation to the sucrose concentrations is important to show the results are flexible with different circumstances. The same volume of sucrose solution will be kept (which is 100ml). The same potato type will be used, different potatoes will differ the results, as the cell properties would not be the same. The weighing scale must be the same and the potato chips should be the same mass, around 10g. The time should stick to its own criteria, either 1 hour or 24 hours. This will be recorded by use of the stopwatch. Safety As with all scientific experiments, safety should be well considered. The experiment should be conducted standing up with safety goggles on. This is to prevent anything going into the person's eyes. ...read more.

Conclusion

Original mass of potato (grams) Average mass of potato (grams) Mass changed of the potato (grams) 0.0 10g 11.15g 1.15g 0.2 10g 10.91g 0.91g 0.4 10g 10.41g 0.41g 0.6 10g 9.98g -0.02g 0.8 10g 9.19g -0.81g 1.0 10g 8.63g -1.37g Analysis Here are the representation of my results in a grapical form. Graph for 1 hour Graph for 24 hours Graph for Change of mass in potatoes for 1 hour Graph for Change of mass in potatoes for 24 hours Conclusion In my conclusion, the potatoes with the lowest concentration gained the most mass, and would become hard relating back to the Turgor theory I stated earlier. In contrast to this, the potatoes in the most concentrated solution lost the most weight thus becoming plasmolysed and limp also relating back to the background I have mentioned earlier. Evaluation In general the experiment was succesful the results were consistent and also were in accordance with the theories made at the start.. The experiment could have been improved by: * More subjects used instead of potatoes * More potatoes * Wider time ranger * Different molarities Using this variety of methods could have improved the experiemnt, however I was generally satisfied with the results of this osmosis experiement. ...read more.

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