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Investigating the effect of concentration on enzyme activity.

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Investigating the effect of concentration on enzyme activity. Introduction Enzymes are biological catalysts. They are generally large proteins made up of several hundred amino acids. Enzymes catalyse thousands of chemical reactions that occur in living cells. Enzymes are highly specific so each one speeds up only one particular chemical reaction. Many kinds of enzymes are found in each cell but because they are used over and over there may be only a small amount of each enzyme present. Safety * Hydrogen Peroxide damages clothes, rinse any spills. * Wear safety goggles to protect eyes from Hydrogen Peroxide. * Report any spills. * Keep scalpel in cork until needed, then replace after use. Variables * Concentration of H2O2. * Concentration of H2O. * Size of potato piece. All other factors will stay the same. ...read more.


Background Knowledge Catalase is found in both plant and animal tissues. It is especially abundant in plant storage organs such as potatoes and the fleshy parts of fruits. Catalase is extremely important in cells because it prevents the accumulation of hydrogen peroxide. Hydrogen peroxide is a strong oxidizing agent, which tends to disrupt the delicate balance of cell chemistry. If too much hydrogen peroxide accumulates, it will kill the cell. Several factors affect the action of enzymes: salt concentration, pH, temperature, enzyme poisons, radiation, the concentration of enzymes, and the concentration of the substance. Catalase accelerates the breakdown of hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2). The chemical equation for this reaction is: 2 H2O2 ----Catalase-------> 2H2O + O2 Prediction I predict that the less diluted the H2O2 solution is the faster the bubbles will travel 5cm up the delivery tube. ...read more.


3.34 8.52 23 7.79 5. 4.56 9.91 32.67 15.7 6. No Reaction No Reaction No Reaction No Reaction Conclusion The graph shows that experiment number one reacted the fastest; we know experiment number one had the highest concentration of H2O2 and 0ml of H2O. So this proves my hypothesis that the less diluted the solution was the faster the reaction, because there is no H2O dilution the solution. Evaluation All of the results fall into the same pattern; the less diluted the solution the quicker the reaction. There where no anomalous results as such but on the graph experiment number 4 reacted 0.01 seconds faster than experiment number 3 but because of the closeness between the two times we could say it was down to human error, and the 0.01 is insignificant. Inaccuracies in the experiment are cause by human error and the fact the test tube was not airtight. The experiment could be improved by using an air tight tube stopping some of the bubbles escaping. Richard Ellis ...read more.

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