Investigation - Examination of bacterial sensitivity on antibiotics.

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Investigation: Examination of bacterial sensitivity on antibiotics.

Planning:

(a) Introduction: The aim of this experiment is to see the effect of antibiotic on the growth and reproduction of three bacteria; Ecoli, Bacillus subtillis, Micrococcus luteus.

(b) Apparatus:

(i) 3 agar petridishes.

(ii) 3 bacteria solution samples (Ecoli, bacillus, micrococcus).

(iii) Marker pen.

(iv) Alcohol.

(v) Beaker.

(vi) Glass spreader.

(vii) 2ml Syringe.

(viii) 3 antibiotic discs.

(ix) Forceps

(x) Bunsen burner.

(xi) Sticky tape.

(xii) Eye protection.

(c) Method:

(i) Take three petridishes and using the marker pen, mark each dish with your name, time and date, and the type of bacteria.

(ii) Take one bottle of the samples of bacteria you want to inoculate e.g. Ecoli and using the 2ml syringe withdraw about 0.5ml of the bacteria. Note that before you withdraw the bacteria from the bottle, flame the neck of the bottle using the Bunsen burner, this is an aseptic technique.

(iii) Using your syringe with the 0.5ml of Ecoli, add it to one of the petridish containing the agar and immediately place back the