• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation into Effect of Surface area on Catalase activity in potato.

Extracts from this document...


Investigation into Effect of Surface area on Catalase activity in potato. Aim: In this investigation, my aim is to find out how the factor Surface area effects Catalase activity in a potato. Introduction Enzymes are globular shaped proteins. They act as catalysts as they help speed up reactions and remain unchanged themselves. Enzymes are composed of amino acids, arranged in chains, which are the n moulded into 3-dimensional shapes. The molecules which the enzymes breakdown, are often referred to as substrates. The shape of the substrate is complimentary to the enzyme's active site. The enzyme has a very specific 3D shape into which only one specificly shaped substrate can fit into it. After the substrate has taken its place in the active site, it is broken down by the enzyme into a product or products. Now, the product(s) have to leave the site as they do not have the complimentary shape anymore. Cells have an enzyme called catalase to breakdown hydrogen peroxide, which can kill the cells, into two safe products. 2H2O2 2H2O + O2 This type of reaction where a molecule is broken down into two smaller products, is called a catabolic reaction. In order to investigate the effect of surface area on the activity of catalase, I am going to measure the volume of oxygen gas released in order to determine the rates of reaction. ...read more.


* Glassware: All glassware must be handled with care. It is easy break, which can obviously cut you. Apparatus such as boiling tubes should be handled with extreme care especially when the contain contents such water. * Hydrogen Peroxide: Hydrogen Peroxide is an oxidising agent. This means that oxygen gas is given off. This can be dangerous in the way that oxygen can assist any burning fires that may start. * Safety goggles: it is necessary to wear safety goggles during the experiment, as Hydrogen peroxide can cause major eye damage if it were to get into your eyes. * Cork borer : the cork borer has a potential hazard itself. While in use, the potato being cut should be held down by your other hand. If not, the borer can slip and cut you. Method In order to complete to this investigation, the following method needs to be followed: * First of all, I will set my apparatus up in a suitable manner * Using the formula 2?rh +[2?r x 2?r], I will work out the surface areas of the cylinders being tested in this investigation: o 1cm in length, 1cm in diameter = 4.71 cm3 o 2cm in length, 1cm in diameter = 7.85 cm3 o 3cm in length, 1cm in diameter = 10.99 cm3 o 4 cm in length, 1cm in diameter = 14.14 cm3 o 5cm in length, 1cm in diameter = 17.27 cm3 * place the ...read more.


In the same way, at a high pH, there would too little H+ ions and this will again disrupt the shape and hence denature the enzyme. The optimum pH for this reaction is 7. > Concentration of catalase: the higher the concentration of catalase, the higher the rate of reaction as more catalase molecules are exposed to the hydrogen peroxide. The larger the number of catalase molecules, the higher the chance of collision between the active sites and substrates. Therefore, the concentration of catalase will be kept the same throughout the experiments as the higher concentrations give a higher rate of reaction. > Concentration of substrate: the higher the concentration of substrate, the higher the rate of reaction. Reason being that with a larger number of substrate molecules, the chance and frequency of the collisions between the reacting particles is increased. I found that 30cm3 of 0.1 hydrogen peroxide provided the most manageable amount of oxygen produced Precision & Reliability To ensure the experiment is precise and provides reliable results: * Repeat the whole experiment 3 times in order to obtain more accurate results. * Use a gas syringe to be enable accurate measuring of oxygen. A gas syringe is used instead of a manometer, which is where O2 bubbles are counted. * If a manometer is used, it could be inaccurate as some of the bubbles may be missed and not counted. They could be miscounted. * The potato cylinders need to be cut precisely, it is necessary for their edges to be cut straight using the cork borer. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Effects of Surface Area on Catalase Activity in Potato.

    5 star(s)

    Another method which will be used in the experiment is to measure the volume of oxygen released from the downward displacement of water. This method is much more accurate as the readings will show the actual volume of oxygen released during the reaction.

  2. Marked by a teacher

    To investigate the effect of substrate concentration on Catalase Activity.

    3 star(s)

    The rate of reaction steadily rose on 100% however there was one anomaly that arose which seemed to influence the results. Evaluation The method to which I conducted the experiment seemed to be quick, efficient and affective, but there had to have been some problems with it which can explain why my results came out the way they did.

  1. The Effect of Surface Area on Catalase Activity in Potato

    If the amount of substrate is restricted, it will limit the rate of reaction. If anymore enzyme is added, there cannot be an increase in the rate of reaction and therefore the graph tails off. Rate of reaction If the amount of substrate is limited, the graph tails off Enzyme

  2. Investagating the Action of the Enzyme Catalase On the Surface Area of a Potato.

    Catalase 2H2O2 O2 (g) + 2 H2O (l) Hydrogen peroxide oxygen + water The catalyst in this experiment, which is catalase, remains unchanged after this reaction. Catalysts also work by providing an easier route for the reaction to follow such as having lower activation energy. If the activation energy is lower, many more of the collisions will have the necessary minimum energy and so produce a reaction.

  1. Influence of pH on the activity of potato Catalase

    right conditions In conclusion, it may be said that pH 7 has the most influence on the activity of potato catalase because of its own properties, which make it most efficient. Evaluation The conclusion can now be stated with confidence as the results and theories produced are substantial for accurate

  2. Osmosis Investigation

    - I will use a washed razor blade to cut the potato tubors to exactly four centimeters. I will remove the potato skin in the process. - I will now place each potato tubor onto filter paper and measure its mass on the weight scale. I will record these readings.

  1. Investigating the influence of pH on the activity of potato catalase

    The errors that could have been caused by the design of the method are factors, such as the size and number of discs, osmosis in the petri dish containing water, partial breakdown of the hydrogen peroxide, loss of oxygen, amount of agitation and the varying temperature at which the experiment was performed.

  2. Osmosis investigation

    Whilst waiting for the reactions to take place, clear up unnecessary apparatus and wipe down the benches. Remember to leave the chips in their solutions for over an hour. 11. After an hour, remove the pins from each potato chip.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work