• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation into the effect of varying concentrations of potassium nitrate solution, on the germination and subsequent root radicle growth of cress seeds.

Extracts from this document...

Introduction

Investigation into the effect of varying concentrations of potassium nitrate solution, on the germination and subsequent root radicle growth of cress seeds. The following investigation is based on seed germination and growth. In this particular study seeds will be subjected to a variety of different solution concentrations, the aim being to find out how this affects their growth and germination. Introduction The germination of seeds is dependent on the presence of three things; a sufficient water supply, a suitable temperature and an appropriate partial pressure of oxygen. Germination also relies on the maturity of the embryo as well as the presence of the three factors listed above. The picture above shows a seed in trans-section. The embryo consists of a shoot (plumule,) a root (radicle) and 1 or 2 seed leaves. The seed's food store is either contained in the endosperm (tissues surrounding embryo) or within the seed leaves. The role of the testa is to enclose and protect the seed's contents. Water, which is absorbed through the microphyle and testa, is essential for activating the enzymes that catalyse the biochemical reactions of germination. When water is absorbed it stimulates the production of gibberelin (a plant growth regulator, synthesised in most parts of the plant.) The gibberelin, in turn, stimulates the synthesis of amylase by the cells in the aleurone layer. ...read more.

Middle

Carrot - high concentration of potassium nitrate 6. Carrot - low concentration of potassium nitrate 7. Carrot - high concentration of copper nitrate 8. Carrot - high concentration of copper nitrate 9. Cress - large volume of solution 10. Cress - small volume of solution 11. Cress - control 12. Carrot - control The lids of the dishes were then replaced and the dishes were placed in a tray lined and covered with wet newspaper. The wet newspaper makes the environment damp so minimising evaporation by decreasing the water potential gradient between inside and outside the dish. Results The seeds were checked after one day and the newspaper had completely dried out. None of the seeds showed any signs of germination. The newspaper was re-dampened and the seeds left for a further 3 days. After day 4 the control groups had fully germinated with the cress seeds having an average root length of 2.75cm and the carrot seeds an average root length of 1.96cm. It was noted that some seeds had slid under the blotting paper and showed no signs of germination. None of the other seeds showed any signs of germination. It was therefore difficult to decide which solution and at what volume to use in the actual experiment. However, due to the success of the controls, it seemed that a volume of 8cm� was adequate. ...read more.

Conclusion

This is because the nitrogen, in fertilisers, is not needed by the plant until the embryo has developed and has begun to photosynthesise. Once the plant is developed, the nitrogen constitutes to essential biological molecules, such as proteins and nucleic acids. Variables Independent Variable - * Concentration of Potassium Nitrate solution; 0.1mols, 0.01mols, 0.001mols, 0.0001mols and 0.00001mols. Dependent Variables - * Germination of seeds * Length of root radicle grown, once germinated * Appearance of a plumule (shoot.) Control Variables - * Temperature - room temperature * Light - Dark * Total volume of solution - 8cm� * Solution used - potassium nitrate * Water used - distilled * Humidity - Quite humid due to wet newspaper * Equipment used - Consistent throughout Equipment Method 1. Lay 7 of the 14 petri dishes out on the table and remove the lids. The remaining 7 will be used in the repeats. 2. Take the blotting paper and scissors and cut 14 circles, of equal diameter, out of the blotting paper. The circles should have a diameter slightly smaller than that of a petri dish, enabling them to fit neatly inside the base of a dish. 3. Using the marker pen, label the dishes with the following: Dish 1 - '0.1 mol, Potassium Nitrate' Dish 2 - '0.01mols Potassium Nitrate' Dish 3 - '0.001 mol, Potassium Nitrate' Dish 4 - '0.0001 mol, Potassium Nitrate' Dish 5 - '0.00001 mol, Potassium Nitrate' Dish 6 - '8 cm� water' Dish 7 - '8cm� Potassium Nitrate' 4. 5. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Green Plants as Organisms section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Green Plants as Organisms essays

  1. Marked by a teacher

    AN INVESTIGATION OF THE DIFFERENT SPECIES OF MAYFLY WITHIN THE POOL & RIFFLE

    4 star(s)

    is more than the critical value I have accept the null hypothesis. "There is no significant difference in the number of Burrowing Mayfly Nymphs found in the pools and riffles" Swimming Mayfly RIFFLE 2 1 9 7 1 3 POOL 2 3 3 6 2 8 Before I could apply

  2. Marked by a teacher

    Advantages and disadvantages of fertiliser.

    often plagued by disease compared to people who get all the nutrients they need. Therefore the gardener must manually fertilize the plants to ensure they have enough food to grow on. Plants which are fertilized often are bigger, stronger and produce more fruits and flowers than plants which are not fertilized.

  1. Determine the water potential of potato tuber cell with the varying affect of solute ...

    0.75 -2370 0.80 -2580 0.85 -2790 0.90 -3000 0.95 -3250 1.00 -3500 (Anonymous results) From looking at table 1, my results show that in general they were very good. However there does seem to be some anonymous results which have been identified on the graph by 1) and 2) .

  2. The effect that different concentrations of salt solution in the water has on strips ...

    cell and outside the cell and therefore there was a steady net flow in both directions and there was no % change. At 0.0 M the percentage change in mass is 19.32%, this is because osmosis makes plant cells swell up if they're surrounded by weak solution and they become turgid.

  1. Investigation into Cress Seeds

    On the other hand, I predict the other brands will have a high selective breeding. This would be due to the human interference I had mentioned in my scientific knowledge. These could possibly be the 'extra curl'. Farmers would get a certain selection from a crop of cress that may

  2. What is the effect on the rate of respiration of yeast cells with glucose ...

    Thermometer To confirm the exact temperature of the water bath. This ensures that the preferred temperature has been reached. Therefore, it means that the results obtained are more accurate and reliable, as the variable is controlled. Glass rod Stirring the solution made when water is added.

  1. An Investigation into Species Diversity with distance along a Pingo.

    results will be tabulated and statistical tests used on them to see the significance of the results obtained. ii) Light Intensity 1. The light intensity will be measured at intervals throughout the day to obtain a true representation of the amount of light each zone receives.

  2. Experiment Report on Best Fertilisers

    wider variety of advantage for plant growth, to wetting agents and that it was easiest to measure/ weight to give a fairer test. Bibliography http://feeco.com/2011/12/07/npk-fertilizer-what-is-it-and-how-does-it-work/- Information given on NPK fertilizer and its components, benefits and other facts. http://www.grahamturf.com/main/reference/images/liquid_vs_granular.pdf- Information about Granular Fertilizers, what they are good for, which plants they are good for and what they are made of.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work