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Investigation to examine the effects of varying tomato juice concentration on the germination of seeds

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Introduction

Investigation to examine the effects of varying tomato juice concentration on the germination of seeds I plan to investigate the effects of varying tomato juice concentration on the germination of seeds. To do this, I am going to put cotton wool soaked in a constant volume of varying concentration tomato juice in photo film pots with a constant number of cress seeds. I will check the seeds every 24 hours to see how many have germinated. Preliminary Work Before doing the main experiment, I investigated the conditions in which I should carry it out. I investigated whether I should use fungicide, and if so, how should I apply it, whether tomato juice inhibits germination, how much liquid and cotton wool I should use and whether I would do the experiment in light or dark conditions. I needed to know if it would change the experiment too much by using fungicide. I knew that the spores in the air might have reacted with the tomato juice to produce fungus that would make it harder to count the germinated seeds, and so would hinder the experiment. The results showed that fungicide did not hinder the experiment too much: Number of seeds germinated: As the results show, the fungicide did not have an adverse effect on the experiment because the pattern of results was the same for those sprayed with fungicide, and those not sprayed. ...read more.

Middle

As the concentration of tomato juice drops, the amount of germination inhibitors will also fall. This means that when the tomato juice is at a lower concentration, more seeds will germinate than if the concentration of tomato juice was higher. A list of the apparatus I plan to use 2 x 10ml Measuring cylinders 1 sheet of Cotton wool 110 ml Tomato juice 110 ml Water 220 Cress seeds Forceps 22 Photo film cases Lab coat Which factors affect how the investigation will work? Volume of tomato juice solution Concentration of tomato juice Light intensity Temperature Amount of cotton wool Space around seeds Water availability Oxygen availability The volume of tomato juice solution will have an effect on how the investigation will work because it determines how much liquid is in the photo film case. It is important because the seeds could be submerged, so not being able to get oxygen, or may not have enough liquid, so drying out. The concentration of tomato juice will have an effect on how the investigation works because it will determine how much germination inhibitor is present. The amount of light may have an effect on how the investigation will work because some seeds need light to germinate; some only germinate in the dark. ...read more.

Conclusion

I will obtain twenty-two photo film cases and cut out forty, two centimetre squared, pieces of cotton wool. I will place a piece of cotton wool in the bottom of each film case. I will then take a 10ml measuring cylinder and put the required amount of tomato juice in it. I will then fill the measuring cylinder up with water to the 10ml mark. I will vary the concentrations as follows: I will repeat the experiment one more time for each tomato juice concentration. Having poured the correct amount into the film case, soaking the cotton wool, I will place 10 cress seeds on top of the cotton wool, which are as evenly spaced as is in the bounds of experimental error. I will then spray the seeds in each of the film cases five times with fungicide. I will then place another piece of two centimetres squared cotton wool on the top of the seeds. I will check the seeds every 24 hours for 4 days. I will remove the top layer of cotton wool with forceps and count the amount of seeds that have germinated in each film case for each concentration, measure the length of shoots and record the results. When measuring the shoot lengths, I will push a thin ruler into the cases. When recording the results, I will take an average of the shoot lengths in each case. ...read more.

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