I predict that the greater the concentrations of water in the external solution the greater the amount of water that enters the potato cells by osmosis. The smaller the concentration of water in the outside solution the greater the amount of water leaves the cells by osmosis.
However, there will be a point where the concentrations of water inside and outside the potato cells are equal. At this point there will be no change in the length, volume and mass of the potato, as the net movement of the water will be zero, no osmosis occurred.
Apparatus
Cork-borer
6 boiling tubes
Boiling tube rack
Measuring cylinder
Scalpel
Ruler
Safety mat
Deionised water
25 cm3 of Sucrose solution of different concentration:
0.1, 0.2, 0.3, 0.4 and 0.5.
Diagram
Method
- Use the cork-borer to cut six pieces of potato cylinders all at the same length (4cm) and diameter. This would have to be done very accurately as a change in the surface area may allow more or less osmosis to occur.
- Separate the six pieces of potatoes into six boiling tubes, five of them containing 25 cm3 of different concentrations of sucrose solutions, as shown in diagram. The test tubes would have to be labelled so there are no mistakes.
- Use the other boiling tube to put 25 cm3 of deionised water into.
- Leave them at room temperature for 24 hours.
- Measure the new lengths of the potato cylinders.
Safety Precautions
This experiment is quite safe but we may have to consider handling the cork-borers and scalpel with care, as they are sharp objects.
Results
*Anomaly
Conclusion
From my results I can see that deionised water and low concentrated sucrose solution affected the size of the potato. In this case the potato got longer. The higher concentrated solution made the potato shrink.
In osmosis the water particles move from an area where there is high concentration of water to an area low concentration of water. Osmosis took place in my experiment in deionised water and low concentrated water as the particles moved through the semi-permeable membrane, from the outside of the potato to the inside, causing the potato to swell.
In low concentration and deionised water the potato expanded because there was more particles of water outside the potato, which were moving in. This happened because there were fewer particles inside the potato. In high concentration of water the amount of solute is low. This is a weak solution. Where there is a semi-permeable membrane the water will move from the area of high concentration to an area of low concentration to separate the two solutions until both sides are equal.
This experiment helped me find out that osmosis occurs between 2 liquid substances with a partially impermeable membrane, and that higher the water concentration is, the larger increase in grams. My prediction was correct as the results worked according to our hypothesis.
Evaluation
I think that the experiment was very successful. I resulted with accurate enough to results from which I was able to plot two informative graphs. There was one anomaly in my results, which is highlighted. This was caused by a groove in one of the potato cylinders this affected my results and made them unreliable, but the anomaly was not used in the results. Other than that I think I took easily enough results for the amount of concentrations that I was using, and the time that I used for the experiment to last was enough to allow sufficient osmosis to occur.
However if I was to criticize my experiment I would leave the potatoes in the liquids for a little while longer so that I could find the saturation and dehydration point and get more accurate and varied results. Another way to improve my results could be to use more accurate equipment for the investigation. For example using callipers for measuring and cutting the potatoes to 4 cm and then measuring the new height of the potatoes after the 24 hours. Callipers are more accurate than rulers; therefore they would give a more accurate result. Overall my investigation went well and my results matched my initial predictions.