• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Study the reaction of Catalase and Hydrogen Peroxide. I am specifically focusing on the substrate concentration and its effect on Oxygen production.

Extracts from this document...


Biology Coursework: Catalase Aim The aim of my investigation is to study the reaction of Catalase and Hydrogen Peroxide. I am specifically focusing on the substrate concentration and its effect on Oxygen production. Variables There are many variables that can affect the experiment, substrate concentration is one of these, it affects the amount of oxygen produced and the time taken for the enzymes to reach their operation ceiling. The enzyme we are using is Hydrogen Peroxide the more you use the higher the rate of reaction and the more oxygen is produced. It would come to a point where the reaction couldn't get any faster because the enzyme would be saturated and wouldn't be able to handle anymore, all the site would be in use. Another variable is surface area this also affects the rate of reaction and the amount of oxygen is produced. The higher the surface area, the higher the rate of reaction. Also more oxygen is produced. The higher the surface area the larger the surface the substrate can act on. Temperature has a big affect on the speed of the reaction. It would speed up the reaction to an optimum temperature where the maximum rate is achieved. This relationship would carry on until the temperature was raised above the optimum then the enzymes would start to denature causing the rate to drop. The amount of potato used would also affect the speed of the reaction. The more potato used the larger amount of enzyme the substrate has to work away at. ...read more.


Method To do the experiment effectively you have to follow a very strict set procedure so that you don't mess the experiment up. You have to make sure everything is exactly the same at the start of each separate process so the investigation is fair and not bias in any way, which would produce bad results. The first thing we had to do was make sure we had sufficient amount of all the equipment we needed. And all of it was clean and had not been used by anybody else. We cut up the potato into its 1cm� pieces and put 4 pieces in the boiling tube. While the potato was waiting to be reacted we put it on tissue to stop anything getting on it which could affect the experiment. We then had to make sure that the potato would be covered by the substrate. We then sealed the boiling tube with the bung and placed the evacuation tube in the beaker full of water. After this we filled the burette with water and placed it in the beaker next to the end of the evacuation tube. We then filled the syringe with the correct concentration of Hydrogen Peroxide and placed it in the hole in the top of the bung this creates an air lock so the pressure is constant. We now got the stop clock ready and started it once the syringe was compressed. Someone had to be holding on to the burette and wait for all the bubbles to pass out of the evacuation tube before we put burette on the tube to start measuring the Oxygen production. ...read more.


Meaning that when we diluted it, it would already be diluted ultimately resulting in completely messed up results, because the reaction would slow and not create the normal amount of oxygen for that concentration. Another reason for the results not really working is because our group split into two groups and one started at one end of the scale and the other at the other end. We also had to spread the experiment over two lessons, which surely must have affected the results. I believe the evidence that I have collected is good for showing that the Rate of Reaction goes up with the substrate concentration. If the measurements we did were all put through a machine and made and the experiment was done under perfect conditions I believe we would have got the expected results and predicted graph. I believe that the results that I have collected are enough to strongly back up my conclusion because they are very accurate as shown by the R� equation. They do show the beginnings of starting to conform with my predicted graph and are only limited by the lack of a higher concentration substrate water solution. The gradient is positive, which exactly what I predicted. I believe to further the evidence I have already discover I would have to do the experiment on more than one occasion and at different times of the year to see if the atmospheric conditions actually effect the results. It would show the true shape of the graph and whether it was actually reliable data. I could also investigate how temperature affects the rate of reaction this would mean I would have a broader view of how the rate is affected by changing conditions. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. An Investigation of the Effect of Copper Sulphate on Catalase Activity.

    Conclusion As has been shown in the 'Analysis' when copper sulphate concentration is increased the rate at which catalase breaks down hydrogen peroxide into oxygen and water is decreased. Therefore the 'Prediction' is correct. The reason for this has been explored in the 'Scientific Knowledge'.

  2. The effect of aspirin on the action of bovine liver catalase

    suggests that the curve would level off before the rate of the breakdown of hydrogen peroxide is zero, this would mean that at the maximum inhibition the reaction would still occur. If this is true it suggests that aspirin may inhibit the enzyme by bonding to it in a

  1. Influence of pH on the Activity of Potato Catalase.

    Reaction = Volume Time The results for the rate of reaction are displayed as follows: pH Average Time/Seconds Rate of Reaction 2.8 104.2 3.39mm�/s 3.5 83.64 4.22mm�/s 4.3 81.90 4.31mm�/s 6.3 22.03 16.03mm�/s 8.3 31.20 11.32mm�/s Average Time Results Graph: Rate of Reaction Results Graph: Conclusion: From the average time

  2. To determine the rate law for a chemical reaction among hydrogen peroxide, iodide and ...

    in the reaction mixture in A = 0.05 mol dm-3 x (25/1000) dm3 / [75+30+25+5+5+10)/1000] dm3 = 8.33x10-3mol dm-3 the concentration of I- in the reaction mixture in C =0.05 mol dm-3 x (50/1000) dm3 / [50+30+50+5+5+10)/1000] dm3 =1.67x10-2mol dm-3 Reaction Mixture Time / s I- concentration in the reaction

  1. Investigate the effect of changing substrate concentration on the rate of the reaction between ...

    Then again, the concentration can only be augmented to a certain extent. Above this limit, the reaction will be able to go no faster. This is because, as the number of substrate particles builds up, the number of collisions between them and the catalase molecules increases.

  2. Factors Affecting Enzyme Activity

    I will then record the amount of gas collected in the gas cylinder at ten second intervals for a period of two minutes. I will then repeat each experiment three times for each concentration of liver. I will record all my results on the table below.

  1. Enzymes - show how substrate concentration affects the rate of reaction for an enzyme ...

    Enzymes as such have a different optimum pH as certain enzyme work best in specific conditions to carry out a function. However a change to pH can alter the shape of the enzyme. As result of this the active site I distorted so no substrate can fit into the active site.


    This can simply be done by shaking the beakers or flasks gently which contains the solutions ensuring nothing spills. 3. Measure out 10ml of full fat cream using a pipette and add into a beaker labelled cream. You must ensure that you wash the pipette as it will be required once measuring other substances.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work