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Study the reaction of Catalase and Hydrogen Peroxide. I am specifically focusing on the substrate concentration and its effect on Oxygen production.

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Biology Coursework: Catalase Aim The aim of my investigation is to study the reaction of Catalase and Hydrogen Peroxide. I am specifically focusing on the substrate concentration and its effect on Oxygen production. Variables There are many variables that can affect the experiment, substrate concentration is one of these, it affects the amount of oxygen produced and the time taken for the enzymes to reach their operation ceiling. The enzyme we are using is Hydrogen Peroxide the more you use the higher the rate of reaction and the more oxygen is produced. It would come to a point where the reaction couldn't get any faster because the enzyme would be saturated and wouldn't be able to handle anymore, all the site would be in use. Another variable is surface area this also affects the rate of reaction and the amount of oxygen is produced. The higher the surface area, the higher the rate of reaction. Also more oxygen is produced. The higher the surface area the larger the surface the substrate can act on. Temperature has a big affect on the speed of the reaction. It would speed up the reaction to an optimum temperature where the maximum rate is achieved. This relationship would carry on until the temperature was raised above the optimum then the enzymes would start to denature causing the rate to drop. The amount of potato used would also affect the speed of the reaction. The more potato used the larger amount of enzyme the substrate has to work away at. ...read more.


Method To do the experiment effectively you have to follow a very strict set procedure so that you don't mess the experiment up. You have to make sure everything is exactly the same at the start of each separate process so the investigation is fair and not bias in any way, which would produce bad results. The first thing we had to do was make sure we had sufficient amount of all the equipment we needed. And all of it was clean and had not been used by anybody else. We cut up the potato into its 1cm� pieces and put 4 pieces in the boiling tube. While the potato was waiting to be reacted we put it on tissue to stop anything getting on it which could affect the experiment. We then had to make sure that the potato would be covered by the substrate. We then sealed the boiling tube with the bung and placed the evacuation tube in the beaker full of water. After this we filled the burette with water and placed it in the beaker next to the end of the evacuation tube. We then filled the syringe with the correct concentration of Hydrogen Peroxide and placed it in the hole in the top of the bung this creates an air lock so the pressure is constant. We now got the stop clock ready and started it once the syringe was compressed. Someone had to be holding on to the burette and wait for all the bubbles to pass out of the evacuation tube before we put burette on the tube to start measuring the Oxygen production. ...read more.


Meaning that when we diluted it, it would already be diluted ultimately resulting in completely messed up results, because the reaction would slow and not create the normal amount of oxygen for that concentration. Another reason for the results not really working is because our group split into two groups and one started at one end of the scale and the other at the other end. We also had to spread the experiment over two lessons, which surely must have affected the results. I believe the evidence that I have collected is good for showing that the Rate of Reaction goes up with the substrate concentration. If the measurements we did were all put through a machine and made and the experiment was done under perfect conditions I believe we would have got the expected results and predicted graph. I believe that the results that I have collected are enough to strongly back up my conclusion because they are very accurate as shown by the R� equation. They do show the beginnings of starting to conform with my predicted graph and are only limited by the lack of a higher concentration substrate water solution. The gradient is positive, which exactly what I predicted. I believe to further the evidence I have already discover I would have to do the experiment on more than one occasion and at different times of the year to see if the atmospheric conditions actually effect the results. It would show the true shape of the graph and whether it was actually reliable data. I could also investigate how temperature affects the rate of reaction this would mean I would have a broader view of how the rate is affected by changing conditions. ...read more.

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