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The effect of antibiotics on the growth of bacteria.

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The effect of antibiotics on the growth of bacteria. The Aim: The aim of this investigation is to find out what effect out of two antibiotics, penicillin and streptomycin has on the growth and multiplication of two different species of bacteria. The two different types of bacteria we will be using are E-coli and micrococcus luteus. The Apparatus: * Four sterilised Agar plates, * Glass spreader, * Masking tape, * Wax pencil, * Lab roll, * A beaker with 70% alcohol, * E- coli (Culture) * Micrococcus luteus (culture), * Penicillin (antibiotics), * Streptomycin (antibiotics). Scientific Knowledge: E-coli: E-coli are a common type of bacteria and are short for the medical term Escherichia coli. This normally lives inside your intestines, where it helps your body break down and digest the food you eat. E. coli bacteria was discovered by a German bacteriologist Theodor Escherich in the 1885.The organism can be found on a small number of cattle farms and can live in the intestines of healthy cattle. But also we need it in our bodies to keep them healthy we pretty much depend on E-Coli in our intestines to provide us with Vitamin K and B complex vitamins which play a vital role in keeping us strong and healthy. ...read more.


There was also no zone of inhibition present as there was no antibiotic disc's present on the plates. The Aseptic Technique: During this investigation we followed the Aseptic technique; this method was used to make sure we did not contaminate the bacteria with any fungi or bacteria that were already present in the air. The Aseptic technique states to do some and all of the following: * Remove or kill micro organisms from hands and objects. * Use sterile equipment and other items. * Decrease the person's risk of exposure to micro organisms. The Aseptic technique refers to washing our hands before and after carrying out the experiment this is so that no bacteria was contaminated and also after the experiment so that no bacteria was left on our hands, we also wore lab coats so that our contaminated clothes were no near the agar plates. Also refers to the use of sterilized equipment, so for us to meet this condition we used Petri dishes that were already sterilised by the means of an hot steam in an autoclave ( this works like a pressure cooker) furthermore we placed a piece of lab roll on to the table we was working on to generate a clean working area, as well ...read more.


My prediction about micrococcus luteus was also correct, that there would be no effect on the bacteria from the penicillin antibiotic but as for the Streptomycin antibiotic will have effect. This also tells me that an illness containing micrococcus lutes will not be cured by the penicillin antibiotic but where as the streptomycin antibiotic we help cues the illness. The Evaluation: If I was to redo this investigation I would spread my bacteria quite close together so that the bacteria growth grew closer together to give me better readings and a clear understating of the bacteria and its effect. Furthermore I would make sure that I do not open the agar plate fully as it may get contaminated as did mine. Due to this major error I did not get an outcome of reliable results and in addition I had to use some one with better results than me. Also the aseptic technique should be used in a suitable and watchful manor to obtain reliable and accurate results. Over all this experiment was not as very successful as I had planned it to be, as I did not achieve accurate results. But on the other hand observing my fellow classmates outcomes I have gained a clear understanding on how bacteria growth is affected by the presence of antibiotics. Dhruvita Patel Unit 6 ...read more.

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This is a well structured and written report.
1. The sources of researched information should be included.
2. Method section should be organized into numbered steps and written in the present tense.
3. The conclusion should attempt to explain the results.
4. The evaluation should suggest further research opportunities.

Marked by teacher Luke Smithen 23/07/2013

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