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The Effect of Temperature on Amylase
- Essay length: 898 words
- Submitted: 04/04/2012
-
Reviewed by:
skatealexia
The first 200 words of this essay...
The Effect of Temperature on Amylase
Experiment 1
Aim
The aim of the experiment is to measure the rate of disappearance of starch from the reaction mixture by using the enzyme amylase.
Method
* Firstly, the water baths were set at temperatures of 20, 30, 50 and 60C. 0C was set within a beaker of ice water.
* Thereafter a boiling tube was taken and 4ml of 1% starch solution was placed inside it. Also 2ml of 0.1% amylase solution was placed in a test tube.
* The labelled pair of tubes were placed in the water bath and kept there for 10 minutes to reach the required temperature.
* During the 10 minutes, 2 drops of iodine solution was placed into each depression of a spotting tile.
* Next the amylase was tipped into the starch and the boiling tube was shaken gently to mix the contents.
* A dropping pipette was used to immediately withdraw a sample of the solution and one drop of it was placed into a spot of iodine on the spotting tile. The colour was recorded.
* The mixture and the
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Review of essay
Rating: 4 star(s)
Response to the question
The response to the question is done well. The experiment is explained clearly and the analysis and evaluation of the results are to a high standard. The candidate should provide a conclusion and perhaps explain the science behind the procedure in greater detail to gain higher marks.
Level of analysis
A range of basic investigations involving the different properties of amylase are investigated, such as the temperature if works best at and how long it takes for amylase to completely break down starch. The analysis of the experiments is described to an accurate and detailed level. The main errors are pointed out in the experiments but to improve the experiments the candidate should consider possible confounding factors and offer solutions to the major problems posed.
Quality of writing
Spelling, grammar and punctuation are to a high level of accuracy throughout the piece. Decimal places are inconsistent in the tables and should all be rounded to to the same number of significant figures to maintain accuracy.
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