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The effect of temperature on respiration.

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Assessed practical The effect of temperature on respiration. Introduction Every living thing respires in some way. There are two forms of respiration, aerobic and anaerobic. Areobic respiration only takes place in the presence of oxygen and is a far more efficient way for complexed organisms to convert carbohydrates into energy. There are four stages in aerobic respiration, Glycolysis, the link reaction, Krebs cycle and oxidative phosphorylation. Anaerobic respiration is far less efficient than aerobic, but can produce low yields of energy quickly. T6he overall equation for respiration is; C6H12O6 + 6O2 6CO2 + 6H2O + energy A respiratory quotient (RQ) is used to show what substrate is being used in respiration, it can also show if anaerobic respiration is occurring. The following equations can be used to calculate RQ: RQ = Volume of carbon dioxide given out in unit of time Volume of oxygen taken in unit of time Or RQ = moles or molecules of carbon dioxide given out Moles or molecules of oxygen taken in Prediction I predict that an increase in temperature will increase the respiration rate. ...read more.


Both tubes are sealed and attached to the u-tubing containing manometer fluid. A respirometer is suitable for measuring the rate of oxygen consumption of small terrestrial invertebrates. The Carbon dioxide produced in respiration is absorbed by a suitable chemical such as soda-lime or Potassium Hydroxide solution. The amount of oxygen used can be measured by reading the level of manometer fluid against the scale. Using a water bath alters the temperature. We did a test run using this equipment; germinating peas were used instead of small invertebrates. Only one test was completed, this was at room temperature no repeats were made. This was due to time restrictions. This test run taught us how to set up the equipment. We compared our results gained to reference book values and decided that they were reasonably reliable. Variables: * The surrounding atmospheric temperature of the water bath must remain constant to prevent anomalous results being obtained. * The atmospheric pressure must remain constant; this is ok because the experiment will take place in the same room all of the time. ...read more.


Set up the equipment as shown in the diagram above 2. Using the syringe, adjust the level of manometer fluid in the u-tubing to ensure that it is the same level at both sides 3. Seal the screw clip and record the level of manometer fluid every two minutes, over a period of 10 minutes. 4. repeat step 3 for the temperatures of 10 C, 20 C(room temperature), 30 C, 40 C and 50 C. These temperatures can be reached by using either a water bath or crushed ice 5. Each test must be repeated 3 times. 6. Record each result in a table and work out the average. Use the results to calculate the RQ values. Risk Assessment * The NaOH is corrosive, so it must be kept away from the invertebrates. Saftey goggles and a lab coat must be worn to protect eyes and clothes from NaOH. * Living creatures must be treated with respect * Any spillages must be mopped up immediately to prevent accidents occurring. alex Sharp Page 1 5/2/2007 Page 1 of 3 ...read more.

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