• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The hypothesis is the optimum pH level for the enzyme Amylase will be pH7 (neutral) due to the natural location being the mouth in the human body.

Extracts from this document...

Introduction

´╗┐Aim To explore the reaction of enzymes throughout various pH levels. Hypothesis The hypothesis is the optimum pH level for the enzyme Amylase will be pH7 (neutral) due to the natural location being the mouth in the human body. Materials used: 1. Bunsen Burner 2. Heatproof Mat 3. Tripod 4. Gauze 5. Pipette 6. Stirring stick 7. Thermometer 8. Measuring cylinder 9. Beaker 10. Spotting tile 11. Stop watch 12. Iodine 13. 0.1ml Hydrochloric Acid 14. 1ml Amylase 15. 2g Starch 16. 200mls Water 17. 10ml pH buffers 18. pH reader 19. Test Tubes 20. Test Tube holder Methodology 1. Place heatproof mat on a flat surface and place Bunsen burner onto the mat, ensure the Bunsen burner is open so the flame is ...read more.

Middle

Once the Starch solution is cooled, use a pipette and place the Starch solution into the Test tube of Amylase and pH buffer. 10. Using the pipette add the solution from the test tube to the spotting tile and iodine. 11. Continue this procedure at 1 minute intervals. 12. Continue to follow each step but using different pH buffers. 13. Record results. Results The temperature of the experiment was at a steady 22 degrees. pH2 pH4 pH6 pH7 pH8 pH10 1 minute black black Red/ brown Light brown black black 2 minutes black black Light brown Iodine black black 3 minutes black black Iodine black black 4 minutes black black black black 5 minutes black black black black 6 minutes black black black black 7 ...read more.

Conclusion

to neutral which is pH7 and broke down basically straight away at pH7 with it being neutral, whereas at the other pH levels it took longer to break down and at 10 minutes the enzyme would naturally de-nature, so would not work at all. The hypothesis is accepted. The optimum pH level for the enzyme Amylase is at pH6 and pH7 (neutral) due to the natural location being the mouth in the human body. Evaluation If the experiment were to be carried out again there are certain things that could be changed these could include: 1. The amount of Iodine drops used. 2. Testing more pH buffers. 3. Testing at a different temperature at a steady rate. 4. Concentration of enzyme. ________________ Michelle WassPage 8 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work