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To investigate the effect of substrate concentration on Catalase Activity.

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Introduction

Biology Coursework Aim: To investigate the effect of substrate concentration on Catalase Activity. Plan: Equipment: Catalase: The Enzyme used to release Oxygen from the hydrogen peroxide solution. The enzyme is present in potatoes/ Metal ruler: This ruler will be used to measure the length of the potato so it can be cut in to equal proportions making the experiment a fair test. Bowl: To keep the measuring cylinder submerged allowing us to measure the amount of water displaced. This will allow us to take results from the experiment. Thermometer: The thermometer will be used to determine the temperature of the water and to keep the temperature constant between each experiment allowing a fair test. Measuring cylinder: The amount of water displaced will be calculated and this will show the amount of oxygen we have in cm3 Bung with plastic tubing: The bung will be used to close off the opening and stop any oxygen escaping. The tubing will lead the oxygen to the upside down measuring cylinder which is located within the bowl. It will stop any oxygen leaking into the atmosphere. Beaker: To measure out the amount of hydrogen peroxide needed. The beaker will also be used to measure out the amount of water needed. ...read more.

Middle

1st Run 1.5 1.0 0.5 1.0 1.0 2nd Run 1.0 2.0 1.0 1.0 1.0 3rd Run 2.0 0.5 1.0 1.0 1.5 50% Concentration of enzyme(Catalase) 1st Run 2.0 0.5 0.5 0.5 1.0 2nd Run 1.0 1.5 1.0 0.5 1.0 3rd Run 0.5 1.0 1.5 1.0 0.5 25% Concentration of enzyme(Catalase) 1st Run 2.0 0.5 0.5 0.5 0.5 2nd Run 1.5 2.0 0.5 0.5 0.5 3rd Run 1.0 2.0 0.5 0.5 0.5 10% Concentration of enzyme(Catalase) 1st Run 0.1 0.2 0.2 0.2 0.3 2nd Run 0.1 0.1 0.2 0.1 0.2 3rd Run 0.1 0.1 0.2 0.2 0.2 Averages Amount of oxygen collected (cm3) Concentration of Catalase Time in minutes 1st minute 2nd minute 3rd minute 4th minute 5th minute Total in 5 minutes 100% 1.1 1.3 2.0 1.3 1.3 7.0 75% 1.1 1.1 0.75 1.0 1.1 5.0 50% 1.1 1.0 1.0 0.75 0.75 4.6 25% 1.5 1.5 0.5 0.5 0.5 4.5 10% 0.1 0.1 0.2 0.1 0.2 0.7 0% 0 0 0 0 0 0 Analysis I noticed that the 100% concentration total of water displaced therefore showing that 7cm3 of oxygen was produced. This total was made with 3 potato discs as with the other experiments. The charts showed that the 10% concentration of hydrogen peroxide produced only 0.7cm3 of oxygen. ...read more.

Conclusion

The experiment was carried out at different times of the day and the temperature changes through out the day, which would change room temperature. The temperature should have been kept the same in order to keep the experiment a fair test. In my research I found out that enzymes work pest at around 40 degrees so we could have kept the temperature of the experiment constantly at 40 degrees in order to use the enzymes to there full potential. To get more accurate results I have made a Modified Procedure: 1. Measure out the correct substrate concentrations ensuring you weigh them to increase accuracy. 2. Use the potato borer to cut the potato strips then cut them into 10x1cm strips with the scalpel. 3. Add the potato then put the stopper on immediately. 4. Measure the amount of oxygen given off into the burette every 30secinds for 5minutes. 5. Repeat the steps five times for each concentration ensuring that you use the same potato apparatus and that the temperature is kept constant preferably at 40 degrees. 6. Ensure that the same person is doing a particular part of the experiment throughout the whole experiment. The reason that this new method will improve the accuracy of my experiment is because I've attacked the issues that I discussed above where possible. ...read more.

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Here's what a teacher thought of this essay

3 star(s)

This is a good attempt at a report on an investigation.
1. The report is missing a title and a researched introduction section.
2. The equipment section is detailed and well written.
3. The method needs to be restructured and the concentrations included.
4. The report is missing a graph.
5. The analysis section is brief and does not use the data to back up the pattern.
6. The evaluation, although detailed, is overly critical and very general.
*** (3 stars)

Marked by teacher Luke Smithen 08/05/2013

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