• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

To investigate the effect of substrate concentration on Catalase Activity.

Extracts from this document...


Biology Coursework Aim: To investigate the effect of substrate concentration on Catalase Activity. Plan: Equipment: Catalase: The Enzyme used to release Oxygen from the hydrogen peroxide solution. The enzyme is present in potatoes/ Metal ruler: This ruler will be used to measure the length of the potato so it can be cut in to equal proportions making the experiment a fair test. Bowl: To keep the measuring cylinder submerged allowing us to measure the amount of water displaced. This will allow us to take results from the experiment. Thermometer: The thermometer will be used to determine the temperature of the water and to keep the temperature constant between each experiment allowing a fair test. Measuring cylinder: The amount of water displaced will be calculated and this will show the amount of oxygen we have in cm3 Bung with plastic tubing: The bung will be used to close off the opening and stop any oxygen escaping. The tubing will lead the oxygen to the upside down measuring cylinder which is located within the bowl. It will stop any oxygen leaking into the atmosphere. Beaker: To measure out the amount of hydrogen peroxide needed. The beaker will also be used to measure out the amount of water needed. ...read more.


1st Run 1.5 1.0 0.5 1.0 1.0 2nd Run 1.0 2.0 1.0 1.0 1.0 3rd Run 2.0 0.5 1.0 1.0 1.5 50% Concentration of enzyme(Catalase) 1st Run 2.0 0.5 0.5 0.5 1.0 2nd Run 1.0 1.5 1.0 0.5 1.0 3rd Run 0.5 1.0 1.5 1.0 0.5 25% Concentration of enzyme(Catalase) 1st Run 2.0 0.5 0.5 0.5 0.5 2nd Run 1.5 2.0 0.5 0.5 0.5 3rd Run 1.0 2.0 0.5 0.5 0.5 10% Concentration of enzyme(Catalase) 1st Run 0.1 0.2 0.2 0.2 0.3 2nd Run 0.1 0.1 0.2 0.1 0.2 3rd Run 0.1 0.1 0.2 0.2 0.2 Averages Amount of oxygen collected (cm3) Concentration of Catalase Time in minutes 1st minute 2nd minute 3rd minute 4th minute 5th minute Total in 5 minutes 100% 1.1 1.3 2.0 1.3 1.3 7.0 75% 1.1 1.1 0.75 1.0 1.1 5.0 50% 1.1 1.0 1.0 0.75 0.75 4.6 25% 1.5 1.5 0.5 0.5 0.5 4.5 10% 0.1 0.1 0.2 0.1 0.2 0.7 0% 0 0 0 0 0 0 Analysis I noticed that the 100% concentration total of water displaced therefore showing that 7cm3 of oxygen was produced. This total was made with 3 potato discs as with the other experiments. The charts showed that the 10% concentration of hydrogen peroxide produced only 0.7cm3 of oxygen. ...read more.


The experiment was carried out at different times of the day and the temperature changes through out the day, which would change room temperature. The temperature should have been kept the same in order to keep the experiment a fair test. In my research I found out that enzymes work pest at around 40 degrees so we could have kept the temperature of the experiment constantly at 40 degrees in order to use the enzymes to there full potential. To get more accurate results I have made a Modified Procedure: 1. Measure out the correct substrate concentrations ensuring you weigh them to increase accuracy. 2. Use the potato borer to cut the potato strips then cut them into 10x1cm strips with the scalpel. 3. Add the potato then put the stopper on immediately. 4. Measure the amount of oxygen given off into the burette every 30secinds for 5minutes. 5. Repeat the steps five times for each concentration ensuring that you use the same potato apparatus and that the temperature is kept constant preferably at 40 degrees. 6. Ensure that the same person is doing a particular part of the experiment throughout the whole experiment. The reason that this new method will improve the accuracy of my experiment is because I've attacked the issues that I discussed above where possible. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

3 star(s)

This is a good attempt at a report on an investigation.
1. The report is missing a title and a researched introduction section.
2. The equipment section is detailed and well written.
3. The method needs to be restructured and the concentrations included.
4. The report is missing a graph.
5. The analysis section is brief and does not use the data to back up the pattern.
6. The evaluation, although detailed, is overly critical and very general.
*** (3 stars)

Marked by teacher Luke Smithen 08/05/2013

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Biology Coursework - Osmosis

    5 star(s)

    Experiment: Method This is the method I followed when conducting my main experiment: 1. Gather together all the required apparatus, and make sure you have plenty of space on your workbench. 2. Place 7 boiling tubes into a test tube rack, using the measuring cylinder measure out 20ml of each concentration and pour them into each boiling tube.

  2. Marked by a teacher

    Isotonic Point of Potatoes

    5 star(s)

    These could have been random or systematic errors, and have been identified below: Possible sources of error: * The mass may not have been measured accurately as the air conditioner was on, which resulted in the continuous fluctuation of the reading on the balance.

  1. Marked by a teacher

    Plan: The effect of the end product, phosphate, on the enzyme phosphatase

    5 star(s)

    So I decide to do three repeats next time. Less than three repeats will not give me enough data to plot error bars, but more than three may be too much during the limited time period(since the enzyme can not maintain their activity very long after been extracted). To make it most efficient, I will use three sets of

  2. Marked by a teacher

    Osmosis in Potato Chips

    5 star(s)

    It is best to wear gloves when using a razor blade but if you cannot just make sure that you are using it properly. Always make sure that you hold it with the blade facing downward and your forefinger and thumb resting on either side of the blunt edge at the top.

  1. A investigation into the effect of inhibitor concentration on the enzyme catalase.

    the substrate in the experiment, it is used to test activity of inhibition. The experiment is dependent on the concentration of Hydrogen Peroxide because if there's more substrate concentration, enzymes work slower than normal, whereas if there's less substrate concentration enzymes work faster, which may vary my results making them not as reliable.

  2. Investigating the effect of changing the concentration of an acid on the rate of ...

    measuring cylinder until the bottom of the meniscus reached the 4cm3 mark. 2. I would collect some 1.0M hydrochloric acid, and squirt it into the measuring cylinder until the bottom of the meniscus reached the 5cm3 mark. Safety In order to make sure the experiment runs smoothly, I need to

  1. Experiment to investigate the effect of Temperature on the enzyme activity of Pectinase

    The inhibitor could inhibit the active site which would reduce the working rate of the enzyme. Inhibitors are found naturally, but are also used as drugs. They are used deliberately to make sure the speed of the reaction is not too quick at certain points.

  2. Experiment investigating concentration of sucrose solution and potatoes

    I obtained a large quantity of accurate results from which I was able to create accurate graphs. HOW I think I took easily enough results for the amount of concentrations that I was using, and the time that I used for the experiment to last was enough to allow sufficient osmosis to occur.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work