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Water Microbiology: a look at a gastrintestinal infection outbreak. Abstract. This investigation is looking at three water samples A, B, and C, which

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Introduction

WATER MICROBIOLOGY: A LOOK AT A GASTRINTESTINAL INFECTION OUTBREAK. ABSTRACT. This investigation is looking at three water samples A, B, and C, which have been associated with a gastrointestinal infection of children in a small village. In which three walls have been associated with the path of infection. The likely cause of outbreak has been identified by using a serial dilution of the samples, which have then been grown on nutrient and Mac Conkeys ager. The growth on Mac Conkeys ager will establish if the organism has come likely from contanated by faecal matter. The growth of the culture was analysed, to try to determine the likely cause and type of organism responsible. The information collected show that the likely cause of infection is from the samples taken from A or B, but further test will have to be carried out to monitor if the organisms or pathogenic or not. Due to sample A and B both having lactose forming bacteria in them, further tests will have to be carried out to determine which is contains the pathogenic bacteria so the best form of treatment can be given, and further further infections can be prevented. INTRODUCTION. There has been an outbreak of a gastrointestinal infection of some young children in a small village, in which the primarily source of infection has been associated three wells on farms which are used for drinking water, which all the infected the children have been to. ...read more.

Middle

Eschericha coli is a gram-negative exteracellur pathogen, which attaches to the intestinal epithelia cells, and causes diarrhoea and dehydration. It is often transmitted by food or water. MATERIALS. > Sterile water samples from well A, B and C. > Sterile Nacl. > Gilson pipette 1000??(variable) > Gilson pipette 100 ? (fixed) > Sterile automatic pipette tips. > Nutrient ager plates. > Mac Conkeys ager plates. > Spreader and alcohol. METHOD. A ten fold serial dilution is produced from each of the three water samples, ranging from 10-1 to 10-6. A 100? measurement of each of the serial dilutions was put on the surface, of a nutrient ager plate, and spread across the ager. Repeat the transferral process of the serial dilutions to the Mac Conkeys ager. Incubate all the samples for forty-eight hours at 37oc. The plates should be inverted before incubation, RESULTS A table, to show the number of colony forming units per I ml of the sample (CFU ml-1) in the nutrient and Mac Conkeys ager. Sample A Sample B Sample C Nutrient ager 2.10 x 108 CFU ml-1 5.60 x 104 CFU ml-1 N/A Mac Conkeys ager 2.90 x 108 CFU ml-1 4.20 x 104 CFU ml-1 N/A Both sample A and B had colonies with fermented lactose, shown by the formation of pink colonies on the Mac Conkeys ager. ANALYSIS The results show that sample C is sterile water as there was no growth on the Mac Conkeys ager, and although there was growth on two of the nutrient ager plates, it is likely that this was due to contamination from outside sources. ...read more.

Conclusion

Once attached, they produce a protein toxin, resulting in loss of water from the cell. The Enteropathogenic Eschericha coli strains, result in malabscrptive diarrhoea. They bind to the intestinal cells by a pili and damages the surface of the cell. They will then form a channel connecting the two cytoplasm in which toxic proteins can be injected. This helps prevent the antibodies produced from the host's immune response from reaching them. Enterohemorrhagic Eschericha coli strains cause malabscriptive diarrhoea. It is the most deadly strain, as they produce a toxin called shiga-like toxin, which will enter the blood stream and cause damage to the kidney, usually by haemorrhages. The children if infected with Eschericha coli, the best form of treatment would be to give them intravenous fluids orally (containing different salts and sugars dissolved in water) to help replace the lost fluid, and reduce further loss. REFERENCE Abigal.A.Salyers and Dixie D.Whitt (2001), microbiology: Diversity, Disease, and the environment, Fitzgerald Science press, first edition, Bethesda Maryland. Michael T. Madigan and John M Martinko(1970), Brock: Biology of Microorganisms, Prentice Hall, Eleventh Edition, Southern Illinois University Carbondale. Allan Jones. Rob Reed, Johnathan Weyers, Practical skills in biology, Third edition, Prentice Hall. Edinburgh gate, Harlow Food Microbiology research group: surveillance and epidemology of antibiotic resistaance in salmonella and E.coli, Prof Blair and Prof McDowel.Tuffs open courseware: http://ocw.tufts.edu/courses/microbiology 14th November 2005. Science education, Oregon state University USA. www.seps.org, 14th November 2005. Dr David Wright ( 2005), Modular Science: Microbial World, Laboratory Handbook and Protocols, University of Teeside: School of Science and Technology. ?? ?? ?? ?? Melanie Pickthall Water Microbiology: A look at a gastrointestinal infection Page 1 ...read more.

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