What Is the Optimum Temperature Of Respiration?

This is called ANAEROBIC REPIRATION but it does not involve oxygen.

All enzymes are needed for a chemical reaction to take place. In this reaction the enzymes are inside the yeast.

Lock and Key

The above diagram is called the lock and key method. It works by the way the enzymes      collisions with the glucose   which then turns in alcohol and carbon dioxide. I think the best temperature will be about 50c because it won’t be boiling but would be cold. The worst temperature I think will be 30c because this is the coldest temperature I think it will be the worse temperature as the enzymes won’t be able to move around and cause a reaction. If this was to happen no bubbles would be let off.

Plan

At first I will collect all the apparatus and set them as shown:

1. I will get a spatula of 2g of yeast and place it in to a beaker and add 5% glucose.
2. The solution will be then mixed and put in the syringe.
3. The syringe will then be placed in the troth at the temperature of the water at 30c this will be repeated with the temperatures 40c 50c 60c and 70c.
4. I will count the amount of bubbles which are let off in the time of one minute using the stop watch for each of the temperatures this is shown to be a fair test because the stopwatch is reliable.
5. These results will then be recorded in my results table .

Safety

Goggles have to be worn during the experiment just in case boiling water gets in to your eyes.

Apparatus

1. Yeast                                   8.Water bath

2. Themometer                       9.Troth

3. Stopwatch

4.5%glucose

5. Beaker

6. Spatula

7. Syringe

Method

At first all the apparatus were collected and set out ready to do the experiment. 1 spatula of yeast was taken and put in to beaker.5% glucose was added to the beaker and this was then stirred to perform a liquid. The liquid was then put in to the syringe and placed in to the troth of the water being at the temperature 30c.This water was taken out of the water bath because the water bath was set at that temperature. The stopwatch was then used to time and count the amount of bubbles that were let off every minute. These results then went in to our results table which then a graph was drawn.

Results Table

min

Conclusion

My results show there is a pattern. This patterns starts at 30c and ends at 60c.The pattern tells me that the rate of bubbles increase because it cause kinetic energy but when it comes to 60c that is its maximum temperature and above 60c the bubbles decrease. This is because the temperature is too hot so the enzymes alter shape so they wouldn’t be able to collisions together to let off carbon dioxide.

Evaluation

My results show they are reliable as they follow a pattern. Therefore I didn’t have any anomalous results this means that the method that I wrote must have been accurate and valid. If I was to do this experiment again I would make sure I tested it in the water bath and not in the troth as in the troth the water can always decrease in temperature but in the water bath the water stays the same temperature. Also if I was to do this experiment again I would also test it in the temperatures 61c 62c 63c 64c onwards as where as it has shown 60c was its maximum, we never tested the temperatures in between 60c and 70c so the pattern could have still increased.