• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Determine the presence of reducing sugar, protein and starch in the food products by using Benedict test, Biuret test and Iodine test,

Extracts from this document...


´╗┐Chapter 1 ? Chemistry of Life Determining the Presence of Certain Nutrients Kieun Bae, DP2 - Biology HL Introduction The idea of this experiment was to plan the diet for a day and determine if the diet plan contains the necessary nutrients, by testing the food products with chemicals. In this way, we would have better idea about what kind of food product should be included in the daily diet. The meal-plan of the day we have made was following: 1. Breakfast 1. milk 2. cereal 1. Lunch 1. bread 2. juice 3. cheese 4. apple 1. Dinner 1. pasta 2. lattice 3. meat 4. tomato 5. yogurt 6. red wine 7. onion This was planned by discussing the actual food products we commonly take in a day. There are few diary products that are commonly known to contain a lot of protein, necessary carbohydrates for each meal (bread, pasta and cereal) as well as the vegetables that contain many various healthy nutrients including glucose (simple sugar), vitamins, minerals etc. Also, there is meat that is supposed to contain some fatty substances and protein. The essential nutrients that we need daily for our body to function properly are mainly six. They are water, carbohydrates (glucose, starch, sucrose etc.), proteins, fats, vitamins and minerals.[1] In the lab, we are going to find out the presence of different nutrients and decide whether the planned list of daily diet food products is good enough to provide the necessary nutrients. The chemical tests that are used for the experiments are: Benedict test, Biuret test and Iodine test, which test for glucose, protein and starch. All the tests indicate if the product contains the certain type of nutrients by the change of colors. One more testing would be HCl/Benedict test, and the purpose of this test will be explained further. Let me explain them, one by one, into details. ...read more.


However, based on what I know and I’ve learned, starch is energy stored in plants, therefore it will not make sense if there is starch present in yogurt which is a dairy product, produced by animals. Also, since the part of an apple we tested was a body of it but not a seed, apple is not supposed to contain starch. The conclusion, therefore, is that the slight difference in colors can be ignored. The reason why they show a little darker yellow color than others might be because of the initial color of the food product or, in case of yogurt, the thickness of the solution (when the substance is thick, the color appears to be matte, darker.). Another confusion that we had in this set of data is Red Wine; it shows purple color. However, since wine is not made of grape seeds but the flesh of grape, it is assumed that there is no starch present. So the purple color is just from the initial color of the wine, dark red. 1. Biuret test Biuret test determines the presence of protein by showing the color purple. The results were following: § Table 3 – Biuret test result Food product Color change Milk light blue Cereal no color change Bread no color change Cheese purple, light clear Forest fruit/strawberry juice a bit more green Apple no color change Pasta no color change Tomato no color change Onion no color change Meat (chicken) greyish blue (dark) Lettuce green Yogurt dark purple Red Wine dark blue-black The result was actually very surprising. We could not see any purple color in the test tube filled with milk! Though, other products that we assumed as protein-containing products had positive result. Dairy products are normally, commonly known to contain a lot of protein. One of the big reasons why many people tend to drink it is, actually, protein! ...read more.


For the next time it would be handy if we try to avoid the products with initial colors that are too strong/dark or similar to the indicating color. Characteristics of different food products were also very hard to overcome. For example, yogurt was too thick to be put into a test tube which made it impossible to put similar amount for the repeat. We tried with the pipette as well as the spoon but it did not work out well. Next time, the method of the experiment should be considered while we choose the products to be tested on. Some products that had a large size had to be ground or cut or broken, and this was also hard to do so since some or them were simply too difficult to cut or break. Chicken meat for example was so hard to cut that we gave up cutting it too small in the end. This might have affected our result because when the particle is too big, due to the surface and volume ratio, it has much less contact with the solution to react. There should be some better materials to improve this (e.g. sharper knife) or some better methods such as using a blender to make the products into smaller particles. The hypothesis was proven to be true through the result of this experiment and even though it was a lab with many lacks and inconveniences, it was very interesting and enjoyable to observe and analyze the reactions. ________________ [1] Essential Nutrients ? AB workouts - http://www.abcworkouts.com/essential-nutrients/ - 21/10/10 [2] Benedict's Test for Reducing Sugars ? Biological Sciences at Ohio University - http://www.biosci.ohiou.edu/introbioslab/Bios170/170_2/benedict.htm - 21/10/10 [3] Simple Biochemical Tests on Carbohydrates - http://www.biotopics.co.uk/as/cho.html - 21/10/10 [4] Biuret Test For Proteins ? SCUM Doctor.com - http://www.scumdoctor.com/nutrition/protein/Biuret-Test-For-Proteins.html - 21/10/10 [5] ?Iodine Test? by Rimlee Bhuyan - 7/23/2010 ? Buzzle.com Intelligent Life on the Web - http://www.buzzle.com/articles/iodine-test.html - 21/10/10 [6] Starch ? Virtual Chembook - http://www.elmhurst.edu/~chm/vchembook/547starch.html - 21/10/10 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Group 4 Projects section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Group 4 Projects essays

  1. Personal Project - creating a website to educate people about the history of the ...

    tone, the acoustic guitar is pleasing to listen to and is still a popular alternative to its electric cousin if the song wants a softer sound although there was a problem. This problem was that the acoustic guitar was a quiet instrument which could hardly be heard when playing alongside different and louder instruments.

  2. Personal project - I have chosen Human Ingenuity as my Area of Interaction because ...

    things and the goal of my project and how to keep these things in mind. It was very challenging at times to remember these steps and I would start complaining because my lips hurt from all the straws or that I kept thinking I couldn't do it because I was running out of room for straws in my mouth.

  1. Comparing the depiction of Indian women in the paintings of RAJA RAVI VARMA during ...

    Raja Ravi Varma painted most of the women from Indian classics such as Kalidasa, Ramayana and Mahabharata. Souza drew his work on the sculptures of Mathura and Khajurao, and found a live specimen to give vent to his erotic and passionate sensualities.

  2. Non-Renewable and Renewable Solutions to Australias Future Electrical Energy Needs

    As coal produces approximately (figure1) of Australia?s current electricity, replacing this with nuclear energy-which is scientifically proven to convert uranium into electricity more efficiently-will help to solve this problem.

  1. Research Report Time management strategies among HKU SPACE Community College students".

    In the time communicating with their family members and friends, the percentages rose rapidly to 55% in both areas. Summarizing the result, we found that males without planning habit have a higher ratio in all areas except the entertainment. This result indicates that the conventional time management methods couldn?t help the males to control the time.

  2. Group 4 Journal. Our process was an industrial one called Aquaponics. Aquaponics is ...

    Usually water enters the bottom of the pump and discharges from the top. Other components of a submersible water pump are the cable, which is connected to the motor, and a pipe that transports the water to the surface of the well.

  1. Advertisment and technology. Planning an ad campaign for Anti-Bully spray

    What to advertise? We all know that adverts don?t care about the product they are advertising. Even the worst of the products are advertised. So, I shouldn?t be too worried about the type of product I?m advertising for this project. Although I did have several ideas in mind.

  2. Designing a series of modern fashion accessories design collection with the theme of combining ...

    . . . . . . 18 5. Result of My Survey . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work