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Investigate How Enzyme Concentration Affects The Rate of Reaction

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Investigate How Enzyme Concentration Affects the Rate of Reaction Null hypothesis: No matter what the concentration of the enzyme; the rate of the reaction will always be the same. Introduction: The aim for the investigation is to find out how enzyme concentration affects the rate of reaction, in this case investigating the breakdown of protein by protease enzymes. The variables that could play a part in the experiment could be the amount of the substances used (5cm), the room/test tube and translucency /substances temperature, or concentration of enzyme. The independent variable was the amount of the substance used as it is measured. The lock and key hypothesis is a simple model of how an enzyme works, the substrate fits into the active site to form a reaction intermediate. The Lock and Key Hypothesis http://www.chemsoc.org/networks/learnnet/cfb/enzymes.htm In this model of the lock and key process, the enzyme molecule changes shape as the substrate closes in. ...read more.


and a milk powder solution (the protein solution). 5cm Pipettes to put the substances into the test tubes. For safety goggles should be worn as chemicals are being used. Also minor fumes may be given off, so be careful not to breathe them in. Avoid spillages onto bare skin as it may be harmful, rinse under cold tap if this is the case, especially in the result of any solution going into a person's eye. Do the experiment at a good pace as not to spill or knock over any fluids or equipment. These safety measures are in place to avoid any harm to people involved in the experiment and to avoid any accidents. 1) Prepare a work space, and then collect the equipment. When collecting the substances be careful as to not let any make contact with the skin, it isn't dangerous but it's advised to be cautious. Also try not to inhale any of the fumes possibly given off during the experiment. ...read more.


Enzyme concentration increases the rate of reaction-higher concentration quicker time to react: This was expected to happen because an enzyme is a biological catalyst and so this speeds up the reaction. Raising the concentration would therefore decrease the time taken for the 2 solutions to mix. Person timekeeping starting at different set off points and stopping at different ending points: This could make the results invalid if a different person was watching and timekeeping during the experiment as peoples reaction times differ. Summary: In this experiment I investigated how enzyme concentration can affect the rate of a reaction. I found out that the higher the enzyme concentration, in this case Trypsin being the enzyme, the quicker the rate of reaction on an average scale. I also found out that the Trypsin turned the Milk powder substance translucent when it mixed in with it. This tells me my Null Hypothesis was wrong and that enzyme concentration does alter the speed of a reaction and that the enzyme used Trypsin was a catalyst in the experiment. ...read more.

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