• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  6. 6
  7. 7
  8. 8
  9. 9
  10. 10
  11. 11
  12. 12
  13. 13

Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with Hydrogen peroxide.

Extracts from this document...


PLANNING EXPERIMENTAL PROCEDURES Title of Investigation: Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with Hydrogen peroxide. 1.Make a Prediction I predict that by increasing the temperature it would speed up the rate of the chemical reaction of the enzyme catalase. The higher the temperature the faster the reaction would occur. As the temperature first gets heated the particles move slowly, as the temperature gradually increases the particles start to move faster and faster and collide more often with enough kinetic energy. 2.Use your knowledge of science to explain why you made the prediction. Enzymes are biological catalyst, which speed up the rate of a Chemical reaction. Enzymes are found in every living cell, different biochemical reactions take place rapidly and simultaneously. An enzyme can be used over and over again without being chemically changed itself. Most enzymes are protein molecule, which are highly specific at catalysing one type of chemical reaction. Without enzymes these reactions would occur too slowly or not at all, and no life would be possible. All living cells make enzymes, but enzymes are not alive. Enzyme molecules function by altering other molecules. Enzymes combine with substrate molecules to form a complex molecular structure in which chemical reactions take place. The enzyme, which remains unchanged, then separates from the product of the reaction. All the reaction that happens inside the living cells goes on at a relatively low temperature. All the reactions that take place in the cells are called metabolic reactions. Every metabolic reaction is controlled by an enzyme, they are controlled in such way that useful products are made and they also get rid of waste materials. The first enzyme to be discovered was amylase, which catalyses the conversion of starch to Maltose. Enzymes work at a suitable temperature and this is known as the Optimum temperature. The enzyme catalase is a Peroxisome which catalyses the breakdown of hydrogen peroxide, a by-product of aerobic respiration. ...read more.


So therefore this suggests that I would have to use temperatures such as 70?c or 80?c to investigate this problem. So it would be very difficult to record these results. If I used the catalase enzyme with a strength of 60 eu/cm3 it will break down the hydrogen peroxide quiet quickly forming the products water and oxygen. I could record the results very easily and this will give me some accurate results. RESULTS TABLE Below is the result table that I have produced to show all my readings from the experiment. Temperature ?c Time it takes for the filter paper to sink and rise back to the top. (S) Average time in seconds Rate of Reaction Rate = 1000 Time taken (s) Run 1 Run 2 Run 3 Run 4 Run 5 Run 6 10 8 9 8 7 8 9 8.2 122 20 8 6 8 9 9 7 7.8 128 30 6 6 5 5 5 5 5.3 189 40 4 5 4 4 5 5 4.5 222 50 4 5 3 3 4 4 3.8 263 60 3 2 3 2 3 3 2.7 370 80 The enzyme denatured, so therefore the filter paper took very, very long to rise back to the top. - ? 0 ANALYSIS As from the results table above it shows that as the average time for each of the temperature decreases, the rate at which these reaction occurs increases. So therefore a simple pattern in the results table could be explained as being, the average time decreases while the rate of reaction increases. The reason why the average time are decreasing is because the temperatures are getting higher so therefore the time it takes for the filter paper to sink to the bottom and rise back up to the top happens very fast due to the change in temperature, and the reason why the rate of reaction increases is because, the temperatures are getting higher each time, and the rate at which these reactions occur ...read more.


We might have gained errors in the practical experiment due to the equipments that we used, in other words the equipments that we used were they accurate enough to measure the solutions of the catalase and the hydrogen peroxide. The errors can also occur due to our ability of using these sorts of equipments, because if we don't know how to use some equipment then we may gain errors in the experiments because of our ability of using them. We must of the ability of using these sorts of equipments in an appropriate experiment. The likely errors that could have occurred in the experiment is that did we measure out the solution of hydrogen peroxide nearest to the top of the beaker, in other words was it almost full? To reduce these sorts of errors we could use more accurate equipments to measure out the solution of hydrogen peroxide for example we could use a burette or a graduated pipette to measure out the hydrogen peroxide or any other solutions. If I was to repeat the whole experiment again, this might enable me to gain better results from the different temperature. As I have stated above that I used the same hydrogen peroxide solution for every temperature that I did, so therefore there may have been some traces of catalase inside the hydrogen peroxide solution when I did different runs on the different temperatures, which may have effected the results so therefore to improve this I will repeat the whole experiment by using a fresh solution of hydrogen peroxide for every different temperatures that I do. By doing this I will hopefully gain some better results. If I repeated the experiment I could use more accurate equipments such as thermostatic water bath to keep the temperature constant, so therefore I will not have to wait for the temperature to come down to the right degrees each time when I do a different temperature, and also use a burette or a graduated pipette to measure out the solutions of hydrogen peroxide. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    The Effect Of Temperature on the Respiration Of Yeast.

    5 star(s)

    * Whether the yeast has been oxygenated (This has to be controlled because if the yeast has been oxygenated then the type of respiration that will take place is aerobic respiration and switch to anaerobic respiration later on in the experiment but if the yeast hasn't been oxygenated then anaerobic

  2. Marked by a teacher

    Beetroot Practical Write up

    3 star(s)

    the four experiments, and therefore accuracy and suitable timing were required, hence a better set of results compared to the other three. The results with the beetroot at 9mm in diameter went up at the end of the graph line; this could be accounted for as not the correct timing or enough accuracy.

  1. Investigating the effects of Copper Sulphate on the action of Catalase Enzyme breaking down ...

    protease enzyme can only break down protein molecules, due to the substrates fitting into the active site. It can not however break down lipids due the lipid substrates having a structure which does not allow it to fit in the active site of the protease enzyme.


    Enzymes activity is affected by: Temperature An increase in temperature, usually results in an increase in the rate of a reactions meaning the enzyme and substrates come together more often. This is because at high temperatures, molecules possess more kinetic energy thus moving around much faster.

  1. Investigating the Effect of Temperature on Rate of Respiration in Yeast

    I believe that methylene blue is the best indicator to use. It is the most reliable and does not require copious amounts of anything, and it doesn't take a long amount of time. A shorter period of time gives a higher reliability.

  2. An Experiment to investigate the affect different temperatures have on the rate of an ...

    However 30�C managed to break down all of the starch into maltose well within the 10 minutes, where as 40�C and 50�C didn't which could suggest that the enzyme molecules might have denatured slightly after being at the higher temperatures for so long.

  1. Investigating the break down of Hydrogen Peroxide using celery tissue to supply the enzyme ...

    Hydrogen peroxide is toxic so needs to be changed into harmless substances. This information was found in textbooks and on Encarta where the functions and uses of enzymes, specifically catalase, were researched. In my investigation I will study the effect of substrate concentration on the rate of catalase activity.

  2. Investigating How Catalase Breaks Down Hydrogen Peroxide.

    This is the 'lock and key' theory, of how enzymes work: a a We can see from above, that the Hydrogen Peroxide molecules have the correct code, and connect on to the catalase enzyme to form an enzyme-substrate complex. The product(s)

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work