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Investigation On Catalase Enzyme.

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INVESTIGATION ON CATALASE ENZYME Introduction I am going to conduct an investigation into how the amount of oxygen is produced by changing one variable effect the decomposition of hydrogen peroxide in the presence of the enzyme. There are many ways in which the amount of oxygen produced could be affected. I believe that surface area of the liver, temperature of the hydrogen peroxide that is reacting, the concentration of the hydrogen peroxide or the amount used are going to be the major variables in the experiment. From these variables I clearly believe that looking at the concentration of the hydrogen peroxide, will be the best experiment to do. Also I believe that this will give me a strong set of results with which I will be able to pull good, clear conclusions from. To keep all other variables constant so they do not influence my results, I will do all experiments in the same room hopefully the temperature remains constant in that one room, all liver will be cut with the same size, as to keep each test fair. Also the volume of the concentration of the hydrogen peroxide will remain at 50 cm�. Prediction I predict that when the concentration of hydrogen peroxide gets lower, the less the reaction would be reduced and the amount of oxygen produced would decrease. A catalyst is a substance which increases the rate of a chemical reaction, without being used up in the process. ...read more.


But because water molecules are now in there as well, the enzymes are finding fewer hydrogen peroxide molecules so there are less reactions taking place. Analysis From the results I had collected I have now plotted a graph, showing the decomposition of the hydrogen peroxide Starting to look at this graph we can clearly see that with the line of best fit drawn the points are not that far away from this line, showing a quite strong relationship. From my graph, I find that the pattern goes up proportionally. One of my experiments shows that the rate of oxygen produced slows down from 0 to 20 seconds. When it reached after 20 seconds the graph shows it was speed up until 30 seconds. That one is experiment 5. Experiment 5 is producing less oxygen than other experiment. I think this is because there are more water molecules in hydrogen peroxide concentration. For experiment 1, I found out that the amount of oxygen produced is increasing from 0 to 30 seconds. I also see that experiment 1 is more producing oxygen than other experiment. I think this is because no water molecules exist in the hydrogen peroxide concentration. Then for experiment 2, the graph shows that the amount of oxygen produced is lower than experiment one but the pattern is still the same. It increases as the time goes by. I think the rate of oxygen produce is lower than experiment 1 because I have put 10cm� water into 40cm� hydrogen peroxide. ...read more.


I can also improve my graph by setting the experiment different from what I have done. For example, I could have collected the gas produced by using a syringe instead of looking at the 250ml cylinder. This could be more accurate. A major cause for concern on accuracy was the reading of the overall measurement of the oxygen produced but also the measurements of hydrogen peroxide and water used for the experiment. So if these miss-readings were carried out through the whole investigation then my results will be quite a long way out. Looking back on experiment I believe that there are many ways to improve it. One way is the accuracy of the measuring of the liver can be done by an electronic ruler, which has a digital screen that shows the length up to 3 decimal places. This would make every liver I use virtually the same length and thickness by using the same size and so this ensures reliability from this. My graph is very basic and one way to improve this will be to increase my range off tests. This will give me more results and so when I plot my graph again there will be more points producing a greater accuracy and a better curve with which we will be able to pull stronger conclusions from. From my experiment, I have found out other factors which might change the result. For example, surface area of the liver, temperature of liquid that is reacting, the concentration of the liquid or the amount used are going to be the major variables in the experiment. ...read more.

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