Materials and method.
Materials.
100ml conical glass
Balancing scale
Measuring boat
Squeezy bottle
Stirring rod
Bunsen burner
Spatula
Method.
Method for making the solution.
- Put the measuring boat onto the scale and reset the number to zero.
- Put the 1g of glucose on the measuring boat using a spatula.
- Put the glucose into the flask.
- Rinse the excess glucose off the boat and the flask.
- Pour water into the flask; fill it up to 100ml from the tap.
- Fill the rest of the flask by using the Squeezy bottle until you get exactly to 100ml.
Method for testing the solution.
- Place a small amount of the 1% solution and add a few drops of benedict’s solution, place the test tube in a beaker of heated water to help the reaction.
- Collect the solution A+B and pour a small amount of each solution into separate test tubes and pour a few drops of benedict’s solution into the mixture.
- Place the test tubes into the heated water and wait for any changes.
- If any changes note them down.
Results.
The control solution changed an orange colour, which means that the glucose was present and that now we be looking for in other tests. Source B stayed the same, which meant that no glucose was present and source A changed colour that shows that the glucose was present
Conclusion.
I think that we got a good result from the test and that we got the right results from what we predicted.
Evaluation.
The test that we did was successful but there were some problems we had while we were doing the test
Human and equipment errors.
Alternative method.
There were a few alternative method we could have used they were;
1.cleaning sticks (clinistix) = the cleaning sticks were accurate because it was able to tell you how much glucose you had inside of you. This can be tested with the fluids inside of you.
2. Volumetric flask = this was used to measure the volume of the solution more accurately.
We could have used the flask but there was the lack of kit and that there was not enough equipment to go around with.